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Factors affecting the abilities of photosynthesis, photorespiration and heat tolerance of plant leaves

Research Project

Project/Area Number 11460072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学
Research InstitutionKYUSHU UNIVETRSITY

Principal Investigator

KOBAYASHI Yoshichika  Faculty of Agriculture, Kyushu University, Prof., 大学院・農学研究院, 教授 (90087594)

Co-Investigator(Kenkyū-buntansha) 中原 光久  (株)九州電力, 電力総合研究所, 研究員
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2001: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥7,400,000 (Direct Cost: ¥7,400,000)
Keywordsphotosynthesis / photorespiration / electron transport / fatty acids / 樹木 / ストレス / 炭酸固定 / RubisCO
Research Abstract

The aim of this study was to determine the physiological factors affecting the abilities of photosynthesis, photorespiration and temperature-stress tolerance of herbaceous plants and woody plants.
Photosynthesis is one of the most heat-sensitive functions of plant cells. Temperatures in the range of 35 ℃ to 45 ℃ tend to inhibit photosynthesis. In order to assess the effect of high temperature on the photosynthetic machinery, the level of photosynthetic activity was measured in transgenic tobacco plants in which the expression of the chloroplast trienoic fatty acid synthetase gene was inhibited. The transgenic plants contained a lower level of trienoic fatty acids than wild-type plants and were better able to acclimate to higher temperature. The ω-3 fatty acid desaturase enzyme, which is expressed in nearly all plant species, may be widely useful in engineering temperature tolerance in plants.
Photosynthetic electron transport is damaged when strong illumination causes either excessive reduction on the acceptor side or oxidation on the donor side of photosystem II (PS II). Plants have protective mechanisms such as photorespiration and down regulation of PS II to prevent the damage. The relationship between photorespiration and the down regulation of PS II was investigated. It was found that the down regulation of PS II is increased with acceleration of photorespiration when the limiting factor of photosynthesis was changed from light to CO_2. From these results, it was concluded that the most important factor affecting photorespiration is the diffusivity of CO_2 in leaf tissue.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Y.Murakami, M.Tsuyama et al.: "Trienolic fatty acids and plant tolerance of high temoerature"Science. 287. 476-479 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] U.Heber et al.: "Protection of the photosynthetic apparatus against damages by excessive illumination in homoiohydric leaves and poikilohydric mosses and lichens"J. Experimental Botany. 52. 1999-2006 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Tsuyama, et al.: "PS-II down regulation as affected by the maximal activity of photorespiration in leaves of C3 plants : suppression of the activity of photorespiration under low light"Proc. of 12th Intl. Congress on Photosynthesis. (In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 共著(矢幡, 小林, 百島他): "巨大クスノキの研究"大宰府天満宮クスノキ養生会議. 234 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Murakami, M. Tsuyama, Y. Kobayashi, H. Kodama and K. Iba: "Trienolic fatty acids and plant tolerance of high temperature"Science. 287. 476-479 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] U. Heber, N.G. Bukhov, V.A. Shuvalov, Y. Kobayashi: "Protection of the photosynthetic apparatus against damages by excessive illumination in homoiohydric leaves and poikilohydric mosses and lichens"J. Experimental Botany. 52. 1999-2006 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Tsuyama, A. Takemiya and Y. Kobayashi: "PS-II down regulation as affected by the maximal activity of photorespiration in leaves of C3 plants : suppression of the activity of photorespiration under low light"Proc. of 12th Intl. Congress on Photosynthesis. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Murakami et al.: "Trienoic fatty acids and plant tolerance of high temperature"Science. 287. 476-479 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] U.Heber, et al.: "Protection of the photosynthetic apparatus against damage by excessive Illumination of homoiohydric leaves and poikilohydric mosses and lichens"J.Experimental Botany. 52・363. 1999-2006 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Tsuyama et al.: "PS II down regulation as affected by the maximal activity of photorespiration In leaves of C3 plants"Photosynthesis. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 共著(矢幡, 小林, 百島 他): "巨大クスノキの研究"太宰府天満宮クスノキ養生会議. 234 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Murakami et al.: "Trienoic fatty acids and plant tolerance of high temperature"Science. 287. 476-479 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] U.Heber,Y.Kobayasi et al.: "Protection of the photosynthetic apparatus against damage by excessive Illumination of homoiohydric leaves and poikilohydric mosses and lichens"Planta. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Tsuyama: "Estimation of the photosynthetic electron flow in intact leaves;effect of mdulated light intensity on the fluorescence parameters Fv/Fm and ΔF/Fm'"Photosynthesis:Mechanisms and Effects. 4069-4072 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Murakami: "Thirnoic fatty acids and plant tolerance of high temperature"Science. 287. 476-479 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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