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Change of Cell Wall Properties in Woody Plant Cells by Low Temperature

Research Project

Project/Area Number 11460075
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionHokkaido University

Principal Investigator

FUJIKAWA Seizo  Hokkaido Univ., Grad. School of Agr., Prof., 大学院・農学研究科, 教授 (50091492)

Co-Investigator(Kenkyū-buntansha) FUNADA Ryo  Hokkaido Univ, Grad. School of Agr., Asso. Prof., 大学院・農学研究科, 助教授 (20192734)
TAKEZAWA Daisuke  Hokkaido Univ. Inst. of Low Temp. Sci., Lec., 低温科学研究所, 助手 (20281834)
ARAKAWA Keita  Hokkaido Univ. Inst. of Low Temp. Sci., Lec., 低温科学研究所, 助手 (00241381)
SANO Yuzou  Hokkaido Univ. Grad. School of Agr., Asso. Prof., 大学院・農学研究科, 助手 (90226043)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsCold adaptation / Freezing behavior / Cell wall / Deep supercooling / Extracellular freezing / Cold-induced proteins / Untifreeze proteins / Gene cloning / 木部放射柔細胞 / 凍結 / 細胞壁結合蛋白質 / シラカンバ / 電子顕微鏡 / 低温環境
Research Abstract

This study concerned to cold temperature adaptation of woody plant cells with the relation to the cell wall properties. This study provided a new evidence by cryo-scanning electron microscopy that xylem parenchyma cells of all hardwood species with thick and rigid cell walls, including boreal hardwood species, responded to subfreezing temperatures by deep supercooling. The mechanism of deep supercooling in xylem parenchyma cells has been studied in previous works and hypothesized to be due to the smaller size of micro-capillaries in the cell walls. However, this study confirmed that there was no distinct change in capillary size of the cell walls between cells exhibiting extracellular freezing arid deep supercooling. This study provided a new evidence that degree of supercooling is distinctly reduced by extraction of soluble substances in xylem cell protoplasts, suggesting the accumulation of antifreeze substances, which might drastically promote deep supercooling. We are currently analyzing candidates of antifreeze proteins and cloning their genes. In addition to these studies, this study also concerned to freezing adaptation mechanisms of cortical parenchyma cells of woody plants, which had comparatively thin soft cell walls and adapted to subfreezing temperature by extracellular freezing. All these results revealed specific physiological response of woody plant cells to environmental stresses as well as suggested the possibility of industrial application of stress-induced proteins from woody plant cells.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] 久保田勝利: "低温馴化によるシラカンバ木部放射柔細胞の細胞壁の変化"低温生物工学会誌. 45. 39-42 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ukaji N: "Accumulation of small-heat shock protein homologues in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation"Plant Physiology. 120. 481-489 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 山田朋義: "低温感受性植物細胞の凍結挙動"低温生物工学会誌. 45. 7-13 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagao M: "Akinete formation in Tribonema bombycinum Derbes et solier (Xanthophyceae) in relation to freezing tolerance"Journal of Plant Research. 112. 163-174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa S: "Determination of the role of cold acclimation-induced diverse changes in plant cells from the viewpoint of avoidance of freezing injury"Journal of Plant Research. 112. 237-244 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa S: "Ultrastructural aspects of freezing adaptation of cells by vitrification"In (Engelmann F & Takagi H eds) Cryopreservation of Tropical Plant Germplasm, Current Research Progress and Application, JIRCAS International Agriculture Series No. 8, International Plant Genetic Resources Institute. 36-42 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ukaji N: "Isolation and characterization of endoplasmic reticulum from mulberry cortical parenchyma cells"In (Dashek W V ed), Methods in Plant Electron Microscopy and Cytochemistry, Humana press Inc, Totawa, NJ. 147-160 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa S: "Cryo-scanning electron microscopic study on freezing behavior of xylem ray parenchyma cells in hardwood species"Micron. 31. 669-686 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤川清三: "樹木の耐凍性をはかる"森林科学. 30. 50 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jitsuyama Y: "Loading process of sugars into cabbage petiole and asparagus shoot apex cells by incubation with hypertonic sugar solutions"Protoplasma. 217. 217 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ukaji N: "Cold acclimation-induced WAP27 localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree was homologous to group 3 late-embryogenesis aboundant proteins"Plant Physiology. 126. 1588-1597 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤川清三: "樹木木部柔細胞の細胞壁構造特性と寒冷適応(総説)"木材学会誌. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kubota M., Arakawa K., Kuroda K., Fujikawa S.: "Changes of cell wall in xylem ray parenchyma cells of birch by cold acclimation"Cryobiology and Cryotechnology. 45. 39-42 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ukaji N., Kuwabara C., Takezawa D., Arakawa K., Yoshida S., Fujikawa, S.: "Accumulation of small-heat shock protein homologues in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation"Plant Physiology. 120. 481-489 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada T., Kuroda K., Jitsuyama Y., Fujikawa S.: "Cryobiology and Cryotechnology"45. 7-13 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagao M., Arakawa K., Takezawa D., Yoshida S., Fujikawa S.: "Akinete Formation in Tribonema bombysnum Derbes et solier (Xanthophyceae) in relation to freezing tolerance"Journal of Plant Research. 112. 163-174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa S., Kuroda K., Jitsuyama Y.: "Determination of the role of cold acclimation-induced diverse changes in plant cells from the viewpoint of avoidance of freezing injury"Journal of Plant Research. 112. 237-244 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa S., Jitsuyama Y.: "Ultrastructural aspects of freezing adaptation of cells by vitrification, In (Engelmann F., Takagi H., Eds) Cryopreservation of Tropical Plant Germplasm"Current Research Progress and Application, JIRCAS International Agriculture Series No. 8, International Plant Genetic Resources Institute. 36-42 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ukaji N., Fujikawa S., Yoshida S.: "Isolation and characterization of endoplasmic reticulum from mulberry cortical parenchyma cells, In Dashek W V ed, Methods in Plant electron microscopy and cytochemistry"Humana press Inc, Totawa. NJ. 147-160 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa S., Kuroda K.: "Cryo-scanning electron microscopic study on freezing behavior of xylem ray parenchyma cells in hardwood species"Micron. 31. 669-686 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa S.: "Measurement of freezing tolerance in trees"Shinrinkagaku. 30. 50-50 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jitsuyama Y., Suzuki T., Harada T., Fujikawa S.: "Loading process of sugars into cabbage petiole and asparagus shoot apex cells by incubation with hypertonic sugar solutions"Protoplasma. 217. 205-216 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ukaji N., Kuwabara C., Takezawa D., Arakawa K., Fujikawa S.: "Cold acclimation-induced WAP27 localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree was homologous to group 3 late-embryogenesis abouondant proteins"Plant Physiology. 126. 1588-1597 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa S.: "Structural property of cell walls in xylem parenchyma cells of trees in relation to the freezing adaptation"Mokuzai Gakkaishi. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤川清三: "樹木の耐凍性をはかる"森林科学. 30. 50 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ukaji N.: "Cold acclimation-induced WAP27 localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree was homologous to group 3 late-embryogenesis aboundant proteins"Plant Physiology. 126. 1588-1597 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jitsuyama Y.: "Loading process of sugars into cabbage petiole and asparagus shoot apex cells by incubation with hypertonic sugar solutions"Protoplasma. 217. 205-216 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 藤川清三: "樹木木部柔細胞の細胞壁構造特性と寒冷適応(総説)"木材学会誌. (印刷中). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山田朋義: "低温感受性植物細胞の凍結挙動"低温生物工学会誌. 45. 7-13 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 久保田勝利: "低温馴化によるシラカンバ木部放射柔細胞の細胞壁の変化"低温生物工学会誌. 45. 39-42 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 長尾学: "黄緑色藻類Tribonema bombycinumのアキネート形成による耐凍性の上昇"低温生物工学会誌. 45. 47-50 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujikawa, S.: "Cryo-scanning electron microscopic study on freezing behavior of xylem ray parenchyma cells in hardwood species"Micron. 31. 669-686 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Jitsuyama, Y.: "Loading process of sugars into cabbage petiole and asparagus shoot apex cells by incubation with hypertonic sugar solutions"Protoplasma. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujikawa, S.: "Ultrastructural aspects of freezing adaptation of cells by vitrification, In (F.Engelmann and H.Takagi Eds.) Cryopreservation of Tropical Plant Germplasm ; Current Research Progress and Application"International Plant Genetic Resources Institute. 36-42 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ukaji, N.: "Isolation and characterization of endoplasmic reticulum from mulberry cortical parenchyma cells, In (W.V.Dashek Ed.) Methods in Plant Electron Microscopy and Cytochemistry"Humana Press Inc.. 147-160 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujikawa, S.: "Freezing adaptation mechanisms of living tissue cells in Betula platyphylla var. japonica Hara and their relation to cell wall properties, In (M.Terazawa Ed.) Tree Sap II"Hokkaido University Press. 93-96 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N. Ukaji: "Accumulation of small heat-shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation"Plant Physiology. 120. 481-489 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Utsumi: "The progression of cavitation in earlywood vessels of fraxinus mandshurica var japonica during freezing and thawing"Plant Physiology. 121. 897-904 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Fujikawa: "Freezing behavior of xylem ray parenchyma cells in softwood species with differences in the organization of cell walls"PROTOPLASMA. 206. 31-40 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Kuroda: "Seasonal changes in the freezing behavior of xylem ray parenchyma cells in four boreal hardwood species"CRYOBIOLOGY. 38. 81-88 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Nagao: "Akinete formation in Tribonema bombycinum Derbes et solier (Xanthophyceae) in relation to freezing tolerance"Journal of Plant Research. 112. 163-174 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Fujikawa: "Determination of the role of cold acclimation-induced diverse changes in plant cells from the viewpoint of avoidance of freezing injury"Journal of Plant Research. 112. 237-244 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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