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NONCULTURE ANALYSES OF MARINE MICROBIAL COMMUNITY IN THE SEDIMENTS USING 16S rRNA GENE

Research Project

Project/Area Number 11460085
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

OHWADA Kouichi  OCEAN RESEARCH INSTITUTION, UNIVERSITY OF TOKYO PROFESSOR, 海洋研究所, 教授 (30013585)

Co-Investigator(Kenkyū-buntansha) WADA Minoru  OCEAN RESEARCH INSTITUTE, UNIVERSITY OF TOKYO RESEARCH ASSOCIATE, 海洋研究所, 助手 (70292860)
NISHIMURA Masahiko  OCEAN RESEARCH INSTITUTE, UNIVERSITY OF TOKYO RESEARCH ASSOCIATE, 海洋研究所, 助手 (10242174)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2000: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1999: ¥8,900,000 (Direct Cost: ¥8,900,000)
Keywordsbiodiversity / marine sediment / nonculture analysis / 16S rDNA / microbial community / 16S rRNA
Research Abstract

To avoid the limitation of traditional culturing techniques for assessing the microbial communities in natural environments, we have successfully developed nonculture analyses of marine microbial communities in the sediments using 16S rRNA gene. Sediment samples were taken from two sampling stations in Sagami Bay, SA and SB, and one station in Tokyo Bay, TK.After DNA extraction from the samples, PCR amplification and cloning of 16S rDNA for the samples was conducted. Then the RFLP patterns were examined after digested with three four-base-specific restriction enzymes (HhaI, RsaI and HaeIII). Thirty-seven clones were selected and sequenced according to dendrograms derived from ARDRA.Sequenced clones fell into five major lineages of the domain Bacteria : the gamma, delta and epsilon Photobacteria. Gram-positive bacteria and the division Verrucomicrobia. At SA, the Verrucomicrobia and the three subclass of the Proteobacteria were found. Most clone sequences belonged to the gamma Proteobacteria. On the other hand, the high GC Gram-positive bacteria and the gamma subslass of the Proteobacteria were common at SB and TK.All clones belonging to the high-GC Gram-positive bacteria collected from SB and TK fell into the same cluster and are regarded as members of an unknown actinomycetes group.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Urakawa,H.,T.Yoshida,M.Nishimura and K.Ohwada: "Characterization of depth-ralated population variation in microbial communities of a coastal marine sediment using 16S eDNA based......"Environmental Microbiology. 2(5). 542-554 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Urakawa,H.,K.Kita-Tsukamoto and K.Ohwada: "Microbial diversity in marine sediments from Sagami Bay and Tokyo Bay Japan, as determined by 16S rRNA gene analysis."Microbiology. 145. 3305-3315 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 浦川秀敏,塚本久美子,大和田紘一: "16S rDNA遺伝子を用いた海洋堆積物における微生物コミュニティーの解析"海洋. 29(12). 750-758 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 浦川秀敏,大和田紘一: "核酸を用いた培養に依存しない微生物群集解析手法"海洋-号外. 23. 176-182 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Urakawa, H., T.Yoshida, M.Nishimura and K.Ohwada.: "Characterization of depth-related population variation in microbial communities of a coastal marine sediment using 16S rDNA based approaches and quinone profiling."Environmental Microbiology. 2(5). 542-554 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Urakawa, H., K.Kita-Tsukamoto and K.Ohwada.: "Microbial diversity in marine sediments from Fagami Bay and Tokto. Bay, Japan, as determined by 16SRNA gence analysis."Microbiology. 145. 3305-3315

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Urakawa, H., K.Kita-Tsukamoto and K.Ohwada.: "Nonculture analysis of marine microbid community using 16S DNA grene."Ocean Monthly. 29(12). 750-758

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Urakawa, H.and K.Ohwada.: "Molecular method for nonculture analysis of microbial Community."Ocean. 23. 176-182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Urakawa,H.,T.Yoshida,M.Nishimura and K.Ohwada: "Characterization of depth-ralated population variation in microbial communities of a coastal marine sediment using 16S eDNA based……"Environmental Microbiology. 2(5). 542-554 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Urakawa,H.,K.Kita-Tsukamoto and K.Ohwada: "Microbial diversity in marine sediments from Sagami Bay and Tokyo Bay, Japan, as determined by 16S rRNA gene analysis."Microbioilogy. 145. 3305-3315 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 浦川秀敏,塚本久美子,大和田紘一: "16S rDNA遺伝子を用いた海洋堆積物における微生物コミュニティーの解析"海洋. 29(12). 750-758 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 浦川秀敏,大和田紘一: "核酸を用いた培養に依存しない微生物群集解析手法"海洋-号外. 23. 176-182 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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