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Biological functions of rhamnose-binding multiple lectins from fish eggs

Research Project

Project/Area Number 11460094
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTohoku University

Principal Investigator

MURAMOTO Koji  Tohoku University, Graduate School of Life Sciences, Professor, 生命科学研究科, 教授 (90157800)

Co-Investigator(Kenkyū-buntansha) KAMIYA Hisao  Kitasato University, School of Fixheries Sciences, Professor, 水産学部, 教授 (80011964)
NAGANUMA Takako  Tohoku University, Graduate School of Life Sciences, Research Associate, 生命科学研究科, 助手 (50250733)
OGAWA Tomohisa  Tohoku University, Graduate School of Life Sciences, Associate Professor, 生命科学研究科, 助教授 (80240901)
SANEYOSHI Mineo  Teikyo University of Science and Technology, Dept. Of Biological Sciences, Professor, 理工学部, 教授 (20002339)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2001: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2000: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1999: ¥6,500,000 (Direct Cost: ¥6,500,000)
KeywordsLectin / Agglutinin / Rhamnose / Salmon / Fish egg / Steelhead trout / Animal lectin / Carbohydrate recognition / 卵 / 低密度リポタンパク質リセプター
Research Abstract

This study focused on the molecular structure, tissue distribution, and biological function of rhamnosebinding lectins (RBLs) isolated from steelhead trout (Oncorhynchus mykiss) eggs. RBLs which were isolated from other Salmonidae such as chum salmon (Oncorhynchus keta) and white-spotted charr (Salvelinus leucomaenis) were characterized for comparison and to know the distribution and evolution of RBLs in fish. Three RBLs, named STL1, STL2, and STL3, were isolated from steelhead trout (Oncorhynchus mykiss) eggs and characterized. These subunits were composed of three (STL1) or two (for STL2 and STL3) tandemly repeated carbohydrate recognition domains (CRDs) in the sequences. Three RBLs named CSL1 CSL2 and CSL3, were isolated from chum salmon eggs and characterized. CSLs are composed of three (for CSL1) or two (for CSL2 and CSLS) tandemly repeated RBL CRDs in the sequences as same as STLs. Two RBLs, named WCL1 and WCL3, were isolated from white-spotted charr eggs. WCL1 and WCL3 showed ap … More proximately 90 % sequence identities to corresponding STL1 and STL3, respectively, and composed of three (for WCL1) or two (for WCL3) tandemly repeated RBL CRDs. STL2-like protein and mRNA were not detected in white-spotted charr. On the basis of the results obtained, the molecular evolution of RBLs and its CRD motif were examined. The CRD motif is not restricted to fishes but is distributed among various other animals, and may have evolved from a common ancestral gene to play their own roles in the biological system. The tissue-specific localization and the changes of STLs proteins and mRNAs during the embryonic development in the steelhead trout were investigated. STL1 has different distribution and expression profiles from those of STL2 and STL3. The interaction between bacteria and STLs was examined. STLs were demonstrated to agglutinate bacteria by recognizing lipopolysacchandes (LPS) and lipoteichoic acid (LTA), which are major integral components of the outer membranes of Gram-negative and Gram-positive bacteria, respectively. This indicates that STLs play important roles in biodefense system. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] H.Tateno: "Immunohistochemical localization of rhamnose-binding lectins in the steelhead trout (Oncorhynchus mykiss)"Develop. Comp. Immunol.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Tateno: "Tissue-specific expression of rhamnose-binding lecctins in the steelhead trout (Oncorhynchus mykiss)"Biosci. Biotechnol. Biochem.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Tateno: "Distribution and molecular evolution of rhamnose-binding lectins in Salmonidae : Isolation and charcterization of two lectins from white-spoted charr (Salvelinus leucomaenis) eggs"Biosci. Biotechnol. Biochem.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Ogawa: "High-level expression and characterization of fully active recombinant conger eel galectins in Eschericia coli"Biosci. Biotechnol. Biochem.. 66. 476-480 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Tateno: "Rhamnose-binding lections from steelhead trout (Oncorhynchus Mykiss) eggs recognize bacterial lipopolysaccharides and lipoteichoic acid"Biosci. Biotechnol. Biochem.. 66. 604-612 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Tateno: "A novel rhamnoes-binding lectin family from eggs of steelhead trout (Oncorhynchus mykiss) with different structures and tissue distribution"Biosci. Biotechnol. Biochem.. 65. 1328-1338 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Kamiya: "Participation of the C-type hemolymph lectin in mineralization of the acom bamacle Megabalanus rosa"marine Biology. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] O.Nakamura: "Galectin containing cells in the skin and mucosal tissues in Japanese conger eel, Conger myriaster. An immunohistochemical study"Develop. Comp. Immunol.. 25. 431-437 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Muramoto: "Comparison of the amino acid sequences of acorn barnacle lectins showing different inhibitory activities toward the crystal growth of calcium carbonate"Fisheries Sci.. 67. 703-709 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Jimbo: "Cloning of the Microcystis aeruginosa M228 lectin (MAL) gene"Biochem. Biophys. Res. Commun.. 273. 499-504 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Yamaguchi: "Effects of culture conditions on the expression level of lectin in Microcystis aeruginosa (Freshwater cyanobacterium)"Fisheries Sci.. 66. 665-669 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 村本光二: "海洋動物レクチンの分子構造と機能に関する研究"日本水産学会誌. 66. 384-387 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Tateno, T. Yamaguchi, T. Ogawa, K. Muramoto, T. Watanabe, H. Kamiya, M. Saneyoshi: "Immunohistochemical localization of rhamnose-binding lectins in the steelhead trout (Oncorhynchus mykiss)"Develop. Comp. Immunol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Tateno, Y. Shibata, Y. Nagahama, T. Hirai, M. Saneyoshi, T. Ogawa, K. Muramoto, H. Kamiya: "Tissue-specific expression of rhamnose-binding lectins in the steelhead trout (Oncorhynchus mykiss)"Biosci. Biotechnol. Biochem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H Tateno, T. Ogawa, K. Muramoto, H. Kamiya, M. Saneyoshi: "Distribution and molecular evolution of rhamnose-binding lectins in Salmonidae : Isolation and characterization of two lectins from white-spoted charr (Salvelinus leucomaenis) eggs"Biosci. Biotechnol. Biochem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Ogawa, C. Ishii, Y. Suda, H. Kamiya, K.Muramoto: "High-level expression and characterization of fully active recombinant conger eel galectins in Eschericia coli"Biosci. Biotechnol. Biochem.. 66(2). 476-480 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Tateno, T. Ogawa, K. Muramoto, H. Kamiya, M. Saneyoshi: "Rhamnose-binding lectins from steelhead trout (Oncorhynchus mykiss) eggs recognize bacterial lipopolysaccharides and lipoteichoic acid"Biosci. Biotechnol. Biochem.. 66(3). 604-612 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H Tateno, T. Ogawa, K. Muramoto, H. Kamiya, T. Hirai, M. Saneyoshi: "A novel rhamnose-binding lectin family from eggs of steelhead trout (Oncorhynchus mykiss) with different structures and tissue distribution"Biosci. Biotechnol. Biochem.. 65(6). 1328-1338 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Kamiya, M. Jimbo, H. Yako, K. Muramoto, O. Nakamura, R. Kado, T. Watanabe: "Participation of the C-type hemolymph lectin in mineralization of the acorn barnacle Megabalanus rosa"Marine Biology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] O. Nakamura, T. Watanabe, H. Kamiya, K. Muramoto: "Galectin containing cells in the skin and mucosal tissues in Japanese conger eel, Conger myriaster. An immunohistochemical study"Develop. Comp. Immunol.. 25(5-6). 431-437 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Muramoto, D.-H. Jin, Y. Niino, K. Fujiwara, S. Kabuto, T. Ogawa, M. Toda, H. Kamiya: "Comparison of the amino acid sequences of acorn barnacle lectins showing different inhibitory activities toward the crystal growth of calcium carbonate"Fisheries Sci.. 67. 703-709 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Jimbo, M. Yamaguchi, K. Muramoto, H. Kamiya: "Cloning of the Microcystis aeruginosa M228 lectin (MAL) gene"Biochem. Biophys. Res. Commun.. 273(2). 499-504 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Yamaguchi, T. Ogawa, K. Muramoto, M. Jimbo, H. Kamiya: "Effects of culture conditions on the expression level of lectin in Microcystis aeruginosa (Freshwater cyanobacterium)"Fisheries Sci.. 66. 665-669 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Muramoto: "Structures and functions of marine animal lectins"Nippon Suisan Gakkaishi. 66(3). 384-387 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Tateno: "Tissue-specific expression of rhamnose-binding lectins in the steelhead trout(Oncorhynchus mykiss)"Biosci. Biotechnol. Biochem. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Tateno: "Distribution and molecular evolution of rhamnose-binding lectins in Salmonidae"Biosci. Biotechnol. Biochem. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ogawa: "Hiah-level expression and characterization of fully active recombinant conger eel galectins in Eschericia coil"Biosci. Biotechnol. Biochem. 66. 476-480 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Tateno: "Rhamnose-bindina lectins from steelhead trout (Oncorhynchus mykiss) eggs recognize bacterial lipopolysaccharides and lipoteichoic acid"Biosci. Biotechnol. Biochem. 66. 604-612 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Tateno: "lmmunohistochemical localization of rhamnose-binding lectins in the steelhead trout (Oncorhynchus mykiss)"Develop. Comp. Immunol. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] O.Nakamura: "Galectin containig cells in the skin and mucosal tissues in Japanese conger eel : An immunohistochemical"Develop.Comp.Immunol.. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Tateno: "A novel rhamnose-binding lectin family from eggs of steelhead trout with different structures and tissue distribution"Biosci.Biotechnol.Biochem.. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Muramoto: "Comparison of the amino acid sequences of acoru barnacle lectins showing different inhibitory activities toward the crystal growth"Fisheries Sci.. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Jimbo: "Cloning of the Microcystis aeruginosa M228 lectin gene"Biochem.Biophys.Res.Commun.. 273. 499-504 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Yamaguchi: "Effects of culture conditions on the expression level of lectin in Microcystis aeruginosa"Fisheries Sci.. 66. 665-669 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 村本光二: "海洋動物レクチンの分子構造と機能に関する研究"日本水産学会誌. 66. 384-387 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masato Yamaguchi: "Isolation and characterization of a mannan-binding lectin from the freshwater cyanobacterium (blue-green algae) Microcystis viridis"Biochem.Biophys.Res.Commun.. 265・3. 703-708 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuyoshi Shirai: "High-resolution structvre of the conger eel galection,congerin I,in lactose-liganded and ligand-free forms"Structure. 7・10. 1223-1233 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masato Yamaguchi: "Purification and characterization of Microcystis aevuginosa (freshwater cyanobacterium) lectin"Comp. Biochem. Physiol. Part B. 123・1. 33-45 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Koji Muramoto: "Functional and structural characterization of multiple galectins from the sgkin mucus of canger eel, Conger myriaster"Comp. Biochem. Physiol. Part B. 123・1. 33-45 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tomohisa Ogawa: "Accelerated evolution in the protein-coding region of galectin cDNAs, Congerin I and Congerin II, from skin mouse of conger eel"Biosci. Biotechnol. Biochem.. 63・7. 1203-1208 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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