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Molecular cloning and expression analysis of acute phase reactants in bony fish.

Research Project

Project/Area Number 11460098
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YANO Tomoki  Faculty of Agriculture KYUSHU UNIVERSITY Professor, 大学院・農学研究院, 教授 (90038266)

Co-Investigator(Kenkyū-buntansha) NAKAO Miki  Faculty of Agriculture KYUSHU UNIVERSITY Assistant Professor, 大学院・農学研究院, 助手 (50212080)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2000: ¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 1999: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsFISH / IMMUNOLOGY / ACUTE PHASE REACTANTS / CLONING / RT-PCR / CARP / TROUT / サイトカイン / 魚類 / 遺伝子 / B因子 / 発現解析
Research Abstract

Complementary DNAs encoding acute phase reactants in the common carp and rainbow trout were cloned by means of the suppression subtractive hybridization. Sodium alginate, turpentaine oil, scleroglucan, and formaline-killed Vibrio anguillarum were used as stimulants. As a result, about 50 genes including acute phase protein known in mammals, such as pentraxin and serum amyloid A, as well as several chemokines, chemokine-receptors, a novel C-type lectin, a novel complement factor B isotypes, glia-maturation factor, IL-1beta, and pre-B cell enhancing factor were identified from carp. On the other hand, 36 genes including LECT2 chemotaxin, complement B/C2, a C-type lectin, fibrinogen, haptoglobin, hemopexine, CD59, lysozyme, viterogenin, precelebrin, Toll-like receptors, transferrin, serum amyloid A, C-polysaccharide-binding protein and thrombin were identified from trout liver. It is intriguing that complement components such as factor B/C2 and CD59, which are expressed constitutively in mammals, showed remarkable increase in their expression levels in response to the stimuli in bony fish, suggesting that some complement components are acute phase reactant in bony fish.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] M Nakao,J Mutsuro,R Obo,K Fujiki,M Nonaka,T Yano: "Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp (Cyprinus carpio) : presence of variants lacking the catalytic histidine"European Journal of Immunology. 30. 858-866 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Nagai,J.Mutsuro,M.Kimura,Y.Kato,K.Fujiki,T.Yano,M.Nakao: "A novel truncated iso form of the mannose-binding lectin-associated serine protease(MASP) from the common carp(Cyprinus carpio)"Immunogenetics. 51. 193-200 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujiki K.,Bayne C.J.,Shin D.-H.,Nakao M.& Yano T.: "Molecular cloning of carp (Cyprinus carpio) C-type lectin and pentraxin by use of suppresion subtractive hybridisation"Fish & Shell fish Immunology. 11(In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Bayne,C.J.,Gerwick L.,Fujiki,K.,Nakao,M.and Yano,T.: "Immune relevant genes identified in the liver of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive hybridization."Developmental and Comparative Immunology. 24(In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujiki T,Shin DH,Nakao M,Yano T: "Molecular cloning and expression analysis of carp (Cyprinus caprio) interleukin-1 beta high affinity immunoglobumin E Fc receptor gamma subunit and serumamyloid A"Fish & Shell fish Immunology. 10. 229-241 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Nakao, J.Mutsuro, R.Obo, K.Fujiki, M.Nonaka and T.Yano: "MOLECULAR CLONING AND PROTEIN ANALYSIS OF DIVERGENT FORMS OF THE COMPLEMENT COMPONENT C3 FROM A BONY FISH, THE COMMON CARP (CYPRINUS CARPIO)"European J.Immunol.. 30. 858-866 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Nagai, J.Mutsuro, M.Kimura, Y.Kato, K.Fujiki, T.Yano and M.Nakao: "A NOVEL TRUNCATED ISOFORM OF THE MANNOSE-BINDING LECTIN-ASSOCIATED SERINE PROTEASE (MASP) FROM THE COMMON CARP (CYPRINUS CARPIO)"Immunogenetics. 51. 193-200 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Fujiki, C.J.Bayne, D.H.Shin, M.Nakao and T.Yano: "MOLECULAR CLONING OF CARP (CYPRINUS CARPIO) C-TYPE LECTIN AND PENTRAXIN BY USE OF SUPPRESSION SUBTRACTIVE HYBRIDIZATION"Fish Shellfish Immunol.. 11 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C.J.Bayne, L.Gerwick, K.Fujiki, M.Nakao and T.Yano.: "IMMUNE RELEVANT GENES IDENTIFIED IN THE LIVER OF RAINBOW TROUT, ONCORHYNCHUS MYKISS, BY MEANS OF SUPPRESSION SUBTRACTIVE HYBRIDIZATION."Dev.Comp.Immunol.. 24. 205-217 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Fujiki, D.H.Shin, M.Nakao and T.Yano.: "MOLECULAR CLONING AND EXPRESSION ANALYSIS OF CARP (CYPRINUS CARPIO) INTERLEUKIN-1B, HIGH AFFINITY IMMUNOGLOBULIN E FC RECEPTOR GAMMA SUBUNIT AND SERUM AMYLOID A"Fish Shellfish Immunol.. 10. 229-241 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M Nakao,J Mutsuro,R Obo,K Fujiki,M Nonaka,T Yano: "Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp (Cyprinus carpio) : presence of variants lacking the catalytic histidine"European Journal of Immunology. 30. 858-866 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nagai,J.Mutsuro,M.Kimura,Y.Kato,K.Fujiki,T.Yano,M.Nakao: "A novel truncated iso form of the mannose-binding lectin-associated serine protease (MASP) from the common carp (Cyprinus carpio)"Immunogenetics. 51. 193-200 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujiki K.,Bayne C.J.,Shin D.-H.,Nakao M.& Yano T.: "Molecular cloning of carp (Cyprinus carpio) C-type lectin and pentraxin by use of suppression subtractive hybridisation"Fish & Shellfish Immunology. 11(In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Bayne,C.J.,Gerwick L.,Fujiki,K.,Nakao,M.and Yano,T.: "Immune relevant genes identified in the liver of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive hybridization."Developmental and Comparative Immunology. 24(In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujiki T,Shin DH,Nakao M,Yano T: "Molecular cloning and expression analysis of carp (Cyprinus caprio) interleukin-1 beta high affinity immunoglobumin E Fc receptor gamma subunit and serumamyloid A"Fish & Shellfish Immunology. 10. 229-241 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakano,M.,Mutruso,L.,Obo.R.,Fujiki,K.,Nonaka,M.,and Yano,T.: "Molecular cloning and protein analysis of dibergent forms of the complemeto component C3 from a bony fisy,the common carp(Cyprinus carpio)."European.Journal of Immunology. 30. 858-866 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Nagai,J.Mutsuo,M.Kimura,Y.Kato,K.Fujiki,T.Yano,and M.Nakao: "A novel truncated isoform of the mannose-binding lectin-associated serine protease (MASP)from the common carp(Cyprinus carpio)."Immunogenetics. 51. 193-200 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Fujiki,D.H.Shin,M.Nakano and T.Yano: "Molecular cloning and expression analysis of the putative carp(Cyprinus carpio)pre-B cell enhancing factor."Fish and Shellfish mmunology. 10(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Fujiki,D.H.Shin,M.Nakano and T.Yano: "Molecular cloning of carp(Cyprinus carpio)CC chemokine,CXC chemokine receptors,allgraft inflammatory factor-1,and nutural killer cell enhancing factor by use of suppression subtractive hybridization.."Immunogenetics. 49. 71-80 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Tomana,M.Nakano,T.Moritomo,K.Fujiki,and t.Yano.: "Isolation of cDNA encoding immunoglobulin light chain from common carp(Cyprinus carpio L.)."Fish and Shellfish mmunology. 9. 909-914 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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