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Multi-dimensional analyses of local regulatory mechanisms in bovine ovary

Research Project

Project/Area Number 11460129
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

OKUDA Kiyoshi  Okayama University, Graduate School of Natural Science andTechnology, Professor, 大学院・自然科学研究科, 教授 (40177168)

Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥9,400,000 (Direct Cost: ¥9,400,000)
Keywordscorpus luteum / prostaglandin / oxytocin / nitric oxide / tumor necrosis factor -α / lipoxygenase / Fas / Fas L system / apoptosis / Hepatocyte growth factor / Fas / FasL system / Estradionl-17β / サイトカイン / レセプター / TNFα / PGF2α / bFGF / アンギオテンシン
Research Abstract

The intra-luteal regulators consist of peptides, eicosanoids, growth factors, cytokines, and neurotransmitters. In the present studies, the roles of locally produced substances in the control of luteal function were demonstrated. The results obtained in this project lead us to the following conclusions:
Luteal progesterone (P4), oxytocin (OT) and PGs produced locally are components of an autocrine/paracrine positive feedback cascade in bovine early to mid-cycle CL, and responsible for the resistance of the early bovine CL to the exogenous PGF2α action.
The presence of functional TNFα receptors was determined not only on the bovine luteal cells obtained cyclic CL and CL of pregnancy, but also on the microvascular endothelial cells derived from developing bovine CL by binding analysis and PCR analyses. Therefore, TNFα may play some roles in regulating bovine CL function throughout the estrous cycle and pregnancy.
LH plays a role in the regulation and maintenance of bovine CL function during … More pregnancy as well as during the estrous cycle, and the effect of LH on CL function depends on the concentration, but not the affinity, of LH receptors.
Adrenergic and nitrergic agents may play opposite roles in the regulation of bovine CL functions. Whereas NA may play a supporting role in luteal development, NO may participate in the functional regression of the bovine CL by inhibiting steroidogenesis.
Estradiol is produced locally in bovine CL throughout the estrous cycle, and estradiol plays a role in regulating PGF2α production in CL as an autocrine/paracrine factor.
Endogenous lipoxygenase products plays important roles in P4 production by bovine CL, i. e. basal P4 production is supported by 12-HETE, and LH-stimulated P4 production is partially mediated via the activation of lipoxygenase and subsequent 15-HETE formation downstream of the LH-activated cAMP-PKA- phosphorylation pathway.
A Fas/Fas L system is present in bovine CL, and leukocyte-derived cytokines play important roles in Fas-mediated luteal cell death. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (62 results)

All Other

All Publications (62 results)

  • [Publications] Dariusz J.Skarzynski: "The sensitivity of bovine corpora lutea to prostaglandin F2α is dependent on and progesterone, oxytocin and prostaglandins"Biology of Reproduction. 60. 1292-1298 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kiyoshi Okuda: "Tumor necrosis factor α receptors in microvascular endothelial cells from bovine corpus luteum"Biology of Reproduction. 61. 1017-1022 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kiyoshi Okuda: "LH receptors during the estrous cycle and pregnancy in the bovine corpus luteum"Reproduction Fertility and Development. 11. 147-151 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 奥田 潔: "黄体と子宮の機能調節におけるアポトーシスの役割とその制御"農林水産技術研究ジャーナル. 22. 27-32 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dariusz J.Skarzynski: "Different actions of noradrenaline and nitric oxide on the output of prostaglandins and progesterone in cultured bovine luteal cells"Prostaglandins & Other Lipid Mediators. 60. 35-47 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ryosuke Sakumoto: "Tumor necrosis factor-α and its receptor in bovine corpus luteum throughout the estrous cycle"Biology of Reproduction. 62. 192-199 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanako Hayashi: "Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from corpus luteum"Biology of Reproduction. 62. 162-167 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ryosuke Sakumoto: "Tumor necrosis factor-α and its receptor in the corpus luteum of pregnant cows"Molecular Reproduction and Development. 55. 406-411 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshihisa Uenoyama: "Basic fibroblast growth factor-induced prostaglandin production and its intracellular mechanisms in cultured bovine luteal cells"Journal of Reproduction and Development. 46. 93-99 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ryosuke Sakumoto: "Tumor necrosis factor-α stimulates prostaglandin F2α secretion by bovine luteal cells via activation of mitogen-activated protein kinase and phospholipase A2 pathways"Molecular Reproduction & Development. 56. 387-391 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dariusz J.Skarzynski: "Influence of nitric oxide and noradrenaline on prostaglandin F2α-induced oxytocin secretion and intracellular calcium mobilisation in cultured bovine luteal cells"Biology of Reproduction. 63. 1000-1005 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kiyoshi Okuda: "Luteal prostaglandin F2 : new concepts of prostaglandin F2α secretion and its actions within the bovine corpus luteum"Asia-Australian Journal of Animal Science. 13. 390-400 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kiyoshi Okuda: "Estradiol-17β is produced in bovine corpus luteum"Biology of Reproduction. 65. 1634-1639 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dariusz J.Skarzynski: "Luteotropic mechanisms in the bovine corpus luteum : role of oxytocin, prostaglandin F2α, progesterone and noradrenaline"Journal of Reproduction and Development. 60. 125-137 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoko Miyamoto: "Tumor necrosis factor-α receptors are present in the corpus luteum throughout the estrous cycle and during the early gestation period in pigs"Reproduction in Domestic Animals. (In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroaki Taniguchi: "Intra-luteal lipoxygenase products modulate progesterone production in cultured bovine luteal cells"Prostaglandins & other Lipid Mediators. (In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Chikako Fujiwara: "Hepatocyte growth factor is an autocrine regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum"Journal of Reproduction and Development. (In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroaki Taniguchi: "Fas/Fas ligand mediates luteal cell death in bovine corpus luteum"Biology of Reproduction. (In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ryosuke Sakumoto: "Possible roles of tumor necrosis factor-α in bovine ovary. In : Miyamoto H, Manabe N (eds), REPRODUCTIVE BIOTECHNOLOGY : Reproductive biotechnology update and its related physiology"Elsevier Scientific Pub., (Tokyo, Japan)(In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jerzy J.Jaroszewski: "Nitric oxide as a local regulator in the mammalian ovary. In : Miyamoto H, Manabe N (eds), REPRODUCTIVE BIOTECHNOLOGY : Reproductive biotechnology update and its related physiology"Elsevier Scientific Pub., (Tokyo, Japan)(In print). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 奥田 潔: "黄体の機能とその制御.「臨床獣医」臨時増刊 「新しい家畜繁殖学」"チクサン出版社,東京(印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Skarzynski DJ, and Okuda K.: "Sensitivity of bovine corpora lutea to prostaglandin F2α is dependent on progesterone, oxytocin and prostaglandins."Biology of Reproduction. 60. 1292-1298 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okuda K, Sakumoto R, Uenoyama Y, Berisha B, Miyamoto A, and Schams D.: "Tumor necrosis factor α receptors in microvascular endothelial cells from bovine corpus luteum."Biology of Reproduction. 61. 1017-1022 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okuda K, Uenoyama Y, Naito C, Sakabe Y, and Kawate N.: "LH receptors during the estrous cycle and pregnancy in the bovine corpus luteum."Reproduction Fertility and Development. 11. 147-151 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Skarzynski DJ, and Okuda K: "Different actions of noradrenaline and nitric oxide on the output of prostaglandins and progesterone in cultured bovine luteal cells."Prostaglandins & other Lipid Mediators. 60. 35-47 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakumoto R, Berisha B, Kawate N, Schams D, and Okuda K.: "Tumor necrosis factor-α and its receptor in bovine corpus luteum throughout the estrous cycle."Biology of Reproduction. 62. 192-199 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi K, Miyamoto A, Berisha B, Kosmann MR, Okuda K, and Schams D: "Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from corpus luteum."Biology of Reproduction. 62. 162-167 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakumoto R, Murakami S, Kishi H, Iga K, Okano A, and Okuda K: "Tumor necrosis factor-α and its receptor in the corpus luteum of pregnant cows."Molecular Reproduction and Development. 55. 406-411 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uenoyama Y, Murakami S, Schams D, and Okuda K: "Basic fibroblast growth factor-induced prostaglandin production and its intracellular mechanisms in cultured bovine luteal cells."Journal of Reproduction and Development. 46. 93-99 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakumoto R, Murakami S, and Okuda K: "Tumor necrosis fector-α stimulates prostaglandin F2α secretion by bovine luteal cells via activation of mitogen-activated protein kinase and phospholipase A2 pathways."Molecular Reproduction and Development. 56. 387-391 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Skarzynski D, Kobayashi S, and Okuda K: "Influence of nitric oxide and noradrenaline on prostaglandin F2α-induced oxytocin secretion and intracellular calcium mobilisation in cultured bovine luteal cells."Biology of Reproduction. 63. 1000-1005 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okuda K, Uenoyama Y, Berisha B, I.G. Lange, H. Taniguchi, S. Kobayashi, S.I. Kobayashi, A. Miyamoto, and D. Schams: "Estradiol-17β is produced in bovine corpus luteum."Biology of Reproduction. 65. 1634-1639 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyamoto Y, Sakumoto R, Sakabe Y, Miyake M, Okano A, and Okuda K: "Tumor necrosis factor-α receptors are present in the corpus luteum throughout the estrous cycle and during the early gestation period in pigs."Reproduction in Domestic Animals. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniguchi H, Uenoyama Y, Miyamoto Y, and Okuda K: "Intra-luteal lipoxygenase products modulate progesterone production in cultured bovine luteal cells."Prostaglandins & other Lipid Mediators. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujiwara C, Murakami S, Taniguchi H, Miyamoto Y, Sakumoto R, Takeuchi S, Takahashi S, and Okuda K: "Hepatocyte growth factor is an autocrine regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum."Journal of Reproduction and Development. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniguchi H, Yokomizo Y, and Okuda K: "Fas/Fas ligand mediates luteal cell death in bovine corpus luteum."Biology of Reproduction. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakumoto R, and Okuda K: "Possible roles of tumor necrosis factor-α in bovine ovary., In: Miyamoto H, Manabe N (eds), REPRODUCTIVE BIOTECHNOLOGY: Reproductive biotechnology update and its related physiology"Elsevier Scientific Pub., (Tokyo, Janan). 113-120 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jaroszewski JJ, Skarzynski DJ, and Okuda K: "Nitric oxide as a local regulator in the mammalian ovary., In: Miyamoto H, Manabe N (eds), REPRODUCTIVE BIOTECHNOLOGY: Reproductive biotechnology update and its related physiology"Elsevier Scientific Pub., (Tokyo, Janan). 105-112 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kiyoshi Okuda: "Estradiol-17β is produced in bovine corpus luteum"Biology of Reproduction. 65. 1634-1639 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Dariusz J.Skarzynski: "Luteotropic mechanisms in the bovine corpus luteum : role of oxytocin, prostaglandin F2α, progesterone and noradrenaline"Journal of Reproduction and Development. 60. 125-137 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] yoko Miyamoto: "Tumor necrosis, factor-α receptors are present in the corpus luteum throughout the estrous cycle and during the early gestation period in pigs"Reproduction in Domestic Animals,. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroaki Taniguchi: "Intra-luteal lipoxygenase products modulate progesterone production in cultured bovine luteal cells"Prostaglandins & other Lipid Mediators. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Chikako Fujiwara: "Hepatocyte growth factor is an antocrine regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum"Journal of Reproduction and Development,. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroaki Taniguchi: "Fas/Fas ligand mediates luteal cell death in bovine corpus luteum"Biology of Reproduction. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ryosuke Sakumoto: "Possible roles of tumor necrosis factor-α in bovine ovary In : Miyamoto H, Manabe N (eds), REPRODUCTIVE BIOTECHNOLOGY : Reproductive biotechnology update and its related physiology"Elsevier Scientific Pub., (Tokyo, Japan)(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jerzy J.Jaroszewski: "Nitric oxide as a local regulator in the mammalian ovary In : Miyamoto H, Manabe N (eds), REPRODUCTIVE BIOTECHNOLOGY : Reproductive biotechnology update and its related physiology"Elsevier Scientific Pub., (Tokyo, Japan)(in Print). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 奥田 潔: "黄体の機能とその制御.「臨床獣医」臨時増刊「新しい畜産繁殖学」"チクサン出版社,東京(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] D.Skarzynski: "Different actions of noradrenaline and nitric oxide on the output of prostaglandins and progesterone in cultured bovine luteal cells."Prostaglandins & other Lipid Mediators. 60. 35-47 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Okuda: "Luteal prostaglandin F2α: new concepts of prostaglandin F2α secretion and its actions within the bovine corpus luteum."Asia-Australian Journal of Animal Science. 13. 390-400 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] D.Skarzynski: "Influence of nitric oxide and noradrenaline on prostaglandin F2α-induced oxytocin secretion and intracellular calcium mobilisation in cultured bovine luteal cells."Biology of Reproduction. 63. 1000-1005 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] R.Sakumoto: "Tumor necrosis factor-α stimulates prostaglandin F2α secretion by bovine luteal cells via activation of mitogen-activated protein kinase and phospholipase A2 pathways."Molecular Reproduction & Development. 56. 387-391 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] D.Skarzynski: "Luteotropic mechanisms in the bovine corpus luteum : role of oxytocin, prostaglandin F2α, progesterone and noradrenaline."Journal of Reproduction and Development. (In Press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] R.Sakumoto: "REPRODUCTIVE BIOTECHNOLOGY AND RELATING PHYSIOLOGY"Possible roles of tumor necrosis factor-α in bovine ovary. In : Miyamto H, Manabe N (eds). Nakanishi Publisher (Kyoto, Japan) (In Print). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Okuda: "REPRODUCTIVE BIOTECHNOLOGY AND RELATING PHYSIOLOGY"Nitric oxide as a local regulator in the mammalian ovary. In : Miyamto H, Manabe N (eds). Nakanishi Publisher (Kyoto, Japan) (In Print). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] D. Skarzynski: "The sensitivity of bovine corpora lutea to prostaglandin F2α is dependent on progesterone, oxytocin and prostaglandins"Biology of Reproduction. 60. 1292-1298 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Okuda: "Tumor necrosis factor α receptors in microvascular endothelial cells from bovine corpus luteum"Biology of Reproduction. 61. 1017-1022 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] D. Skarzynski: "Different actions of noradrenaline and nitric oxide on the output of prostaglandins and progesterone in cultured bovine luteal cells"Prostaglandins & other Lipid Mediators. 60. in press (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] R. Sakumoto: "Tumor necrosis factor-α and its receptor in bovine corpus luteum throughout the estrous cycle"Biology of Reproduction. 62. 192-199 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Hayashi: "Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from corpus luteum"Biology of Reproduction. 62. 162-167 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Okuda: "LH receptors during the estrous cycle and pregnancy in the bovine corpus luteum."Reproduction Fertility & Development. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] R. Sakumoto: "Tumor necrosis factor-α and its receptor in the corpus luteum of pregnant cows"Molecular Reproduction & Development. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Uenoyama: "Basic fibroblast growth factor-induced prostaglandin production and its intracellular mechanisms in cultured bovine luteal cells"Journal of Reproduction & Development. (in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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