Construction of detection system for prion protein using recombinant chicken monoclonal antibody
| Project/Area Number |
11460138
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Hirosima University |
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Principal Investigator |
MATSUDA Haruo Hirosima University, Faculty of Applied Biological Science, Immunobiology, Professor, 生物生産学部, 教授 (80116863)
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| Co-Investigator(Kenkyū-buntansha) |
FURUSAWA Shuichi Hirosima University, Faculty of Applied Biological Science, Immunobiology, Associate Professor, 生物生産学部, 助教授 (80130037)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2000: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | Chicken / Monoclonal antibody / prion disease / phage displayed antibody |
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| Research Abstract |
The chicken is a useful animal for the development of the specific antibodies against the mammalian conserved proteins. In this study, we generated construction of expression vector for chicken single chain fragment of variable region (scFv), named as pCPDS, and two types of chicken scFv antibodies specific for prion protein. In the Western blotting and ELISA analysis, the recombinant monoclonal antibodies were reactive to prion protein from mouse brains. In the foundamental examination for immuno-PCR using phage displayed chicken monoclonal antibody, furthermore, these antibodies were detectable to prion protein peptide with high specificity.
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Report
(3 results)
Research Products
(12 results)
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[Publications] Nakamura, N., Aoki, Y., Horiuchi, H., Furusawa, S., Yamanaka, H., Kitamoto, T., and Matsuda, H.: "Construction of recombinant monoclonal antibodies from a chicken hybridoma line secreting specific antibody"Cytotechnol.. 31. 1-8 (2000)
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[Publications] Kitao, H., Arakawa, H., Kuma, K., Yamagishi, H., Nakamura, N., Furusawa, S., Matsuda, H., Yasuda, M., Ekino, S., and Shimizu, A: "Class switch recombination of the chicken immunoglobulin heavy chain genes : implications for the primordial switch region repeats"Int. Immunol.. 12. 959-968 (2000)
Description
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Related Report
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