THE ROLE OF MACROPHAGES ON THE PATHOGENESIS OF BABESIOSIS

• Project/Area Number: 11460142
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: HOKKAIDO UNIVERSITY
• Principal Investigator: MAEDE Yoshimitsu Hokkaido Univ., Grad. School of Vet. Med., Prof., 大学院・獣医学研究科, 教授 (40002084)
• Co-Investigator(Kenkyū-buntansha): YAMASAKI Masahiro Hokkaido Univ., Grad. School of Vet Med., Inst., 大学院・獣医学研究科, 助手 (40322846) ; YAMATO Osamu Hokkaido Univ., Grad. School of Vet. Med., Lec., 大学院・獣医学研究科, 講師 (80261337)
• Project Period (FY): 1999 – 2001
• Project Status: Completed (Fiscal Year 2001)
• Budget Amount: ¥13,800,000 (Direct Cost: ¥13,800,000); Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000); Fiscal Year 1999: ¥6,600,000 (Direct Cost: ¥6,600,000)
• Keywords: BABESIA / ERYTHROCYTE / OXIDATIVE DAMAGE / SUPEROXIDE / LIPID PEROXIDATION / マクロファージ / 活性酸素

Research Abstract

The present study was carried out to elucidate the mechanisms underlying the oxidative process in Babesia gibsoni-infected erythrocyte(RBC), especially the role of macrophage in the process. As the results, the generation of superoxide anion and the oxidative damage in RBC of dogs infected with B. gibsoni was significantly higher than those in control RBC. When B. gibsoni was cultured together with RBC, superoxide generation of RBC was higher in the parasitized culture than the control culture. The macrophages of infected dogs produced a higher amount of superoxide than those of non-infected dogs, when the cells were subjected to infected dog RBC membrane opsonized with infected dog serum. The membrane lipid peroxidati of RBC incubated with macrophages from infected dogs was higher than that from non-infected dogs. These results suggest that the oxidative damage of RBC in B. gibsoni-infected dogs with a low parasitemia might be induced by superoxide released from the activated macrophages.

Research Products

• [Publications] Y.Otsuka: "Increased generation of superoxide in erythrocytes infected with Babesia gibsoni"J. Vet. Med. Sci.. 63(10). 1007-1081 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.Otsuka: "The effect of macrophages on the erythrocyte oxidative damage and the pathogenesis of anemia in Babesia gibsoni-infected dogs with low parasitemia"J. Vet. Med. Sci.. 64(3). 221-226 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y. Otsuka et. al: "Increased generation of superoxide in erythrocytes infected with Babesia gibsoni"J. Vet. Med. Sci.. 63(10). 1077-1081 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Y. Otsuka et al.: "The effect of macrophages on the erythrocyte oxidative damage and the pathogenesis of anemia in Babesia gibsoni-infected dogs with low parasitemia"J. Vet. Med. Sci.. 64(3). 221-226 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Y. Otsuka: "Increased generation of superoxide in Erythrocytes infected with Babesia gibsoni"J. Vet. Med. Sci.. 63(10). 1077-1081 (2001)
• [Publications] Y. Otsuka: "The effect of macrophages on the erythrocyte oxidative and damage and the Pathogenesis of anemia in Babesia gibsoni-infected dogs with low parasitemia"J. Vet. Med. Sci.. 64(3). 221-226 (2002)