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Molecular analysis of genetic diversity in natural populations of beech species

Research Project

Project/Area Number 11460149
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 生物資源科学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MIKAMI Tetsuo  Hokkaido Univ., Grad.School of Agr., Prof., 大学院・農学研究科, 教授 (50133715)

Co-Investigator(Kenkyū-buntansha) KUBO Tomohiko  Hokkaido Univ., Grad.School of Agr., Inst., 大学院・農学研究科, 助手 (40261333)
KISHIMA Yuji  Hokkaido Univ., Grad.School of Agr., Asso.Prof., 大学院・農学研究科, 助教授 (60192556)
KOIKE Takayoshi  Hokkaido Univ., Grad.School of Agr., Prof., 農学部・附属演習林, 教授 (10270919)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2000: ¥5,200,000 (Direct Cost: ¥5,200,000)
Keywordsbeech / mitochondrial genome / RFLP / genetic diversity / genetic vulnerability / molecular ecology / F.hayatae / evolutionary lineage / 地理的クライン / イヌブナ
Research Abstract

The amount and distribution of mitochondrial (mt) DNA restriction fragment length polymorphism was determined among individual tree samples of two Japanese beech species, Fagus crenata and F.japonica. Individual plants were collected from 16 F.crenata populations throughout the range of the species, and from three F.japonica populations. We detected enough variation to characterize eleven and three chondriome types in F.crenata and F.japonica, respectively. The grouping of beech chondriome types based upon the cladistic analysis of mtDNA polymorphism allowed us to recognize the apparent geographical patterns of mtDNA diversity : the resulting three main groups occupied distinct geographic areas. This geographic differentiation is likely to reflect the history of the Japanese beech forests after the last glacial period of the Pleistocene. In addition, the mtDNA polymorphism encountered within F.crenata encompassed all the variation observed in F.japonica. Our result suggests the need for re-evaluation of their phylogenetic relationships.
MtDNA restriction fragment length polymorphisms were also used to examine cytoplasmic diversity within a relic-like population of F.hayatae, located in northern Taiwan. The analysis failed to reveal any polymorphisms, an observation that suggests cytoplasmic uniformity in the F.hayatae population examined. It is also interesting to note that the chondriome type of our F.hayatae samples is very close to that characteristic of F.crenata populations in the southernmost area of Japan.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] S.Nishizawa: "Variable number of tandem repeat loci in the mitochondrial genomes of beets."Current Genetics. 37. 34-38 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Kubo: "The nad4L-orf25 gene cluster is conserved and expressed in sugar beet mitochondria."Theoretical and Applied Genetics. 100. 214-220 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Kubo: "The complete nucleotide sequence of the mitochondrial genome of sugar beet (Beta vulgaris L.) reveals a novel gene for tRNACys (GCA)."Nucleic Acids Research. 28. 2571-2576 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kato: "The chloroplast genomes of azuki bean and its close relatives : a deletion mutation found in weed azuki bean."Hereditas. 132. 43-48 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kato: "Analysis of mitochondrial DNA of an endangered beech species, Fagus hayatae Palibin ex Hayata."New Forests. 19. 109-114 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikami: "Molecular analysis of cytoplasmic male sterility in beets."Acta Horticulturae. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Kubo: "Alterations in organization and transcription of the mitochondrial genomes of cytoplasmic sterile sugar beet (Y.OnoderaL.)"Molecular and General Genetics. 262. 283-290 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Onodera: "Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet."Journal of Plant Physiology. 155. 656-660 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Kishima: "Structural conservation of the transposon Tam3 family in Antirrhinum majus and estimation of the number of copies able to transpose."Plant Molecular Biology. 39. 299-308 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Yamashita: "Resistance to gap repair of the transposon Tam3 in Antirrhinum majus ; a role of the end regions."Genetics. 153. 1899-1908 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] J.Abe: "Introgression between wild and cultivated soybeans of Japan revealed by RFLP analysis for chloroplast DNAs."Economic Botany. 53. 285-291 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 三上哲夫: "花-性と生殖の分子生物学(日向康吉編)"学会出版センター. 258 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 三上哲夫: "栽培植物の自然史(山口裕文編)"北海道大学図書刊行会(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Nishizawa et al.: "Variable number of tandem repeat loci in the mitochondrial genomes of beets."Current Genetics. 37. 34-38 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Kubo et al.: "The nad4L-orf25 gene cluster is conserved and expressed in sugar beet mitochondria."Theoretical and Applied Genetics. 100. 214-220 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Kubo et al.: "The complete nucleotide sequence of the mitochondrial genome of sugar beet (Beta vulgaris L.) reveals a novel gene for tRNA Cys (GCA)."Nucleic Acids Research. 28. 2571-2576 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kato et al.: "The chloroplast genomes of azuki bean and its close relatives : a deletion mutation found in weed azuki bean."Hereditas. 132. 43-48 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kato et al.: "Analysis of mitochondrial DNA of an endangered beech species, Fagus hayatae Palibin ex Hayata."New Forests. 19. 109-114 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikami et al.: "Molecular analysis of cytoplasmic male sterility in beets."Acta Horticulturae. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Kubo et al.: "Alterations in organization and transcription of the mitochondrial genomes of cytoplasmic male sterile sugar beet (Beta vulgaris L.)"Molecular and General Genetics. 262. 283-290 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Onodera et al.: "Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet."Journal of Plant Physiology. 155. 656-660 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Kishima et al.: "Structural conservation of the transposon Tam3 family in Antirrhinum majus and estimation of the number of copies able to transpose."Plant Molecular Biology. 39. 299-308 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Yamashita et al.: "Resistance to gap repair of the transposon Tam3 in Antirrhinum majus ; a role of the end regions."Genetics. 153. 1899-1908 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] J.Abe et al.: "Introgression between wild and cultivated soybeans of Japan revealed by RFLP analysis for chloroplast DNAs."Economic Botany. 53. 285-291 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikami, (Ed.Kokichi Hinata): "Flawer-Moleanlay Biology on Sexuality and Keprodnetion"Japan Scientific Societieo Preso. 258 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikami, (Ed.Hirofumi Yamaguchi): "Natural History of cultivated plants"Hokkaido University Press (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Nishizawa: "Variable number of tandem repeat loci in the mitochondrial genomes of beets."Current Genetics. 37. 34-38 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Kubo: "The nad4L-orf25 gene cluster is conserved and expressed in sugar beet mitochondria."Theoretical and Applied Genetics. 100. 214-220 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Kubo: "The complete nucleotide sequence of the mitochondrial genome of sugar beet (Beta vulgaris L.) reveals a novel gene for tRNACys (GCA)."Nucleic Acids Research. 28. 2571-2576 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Kato : "The chloroplast genomes of azuki bean and its close relatives : a deletion mutation found in weed azuki bean."Hereditas . 132. 43-48 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Kato: "Analysis of mitochondrial DNA of an endangered beech species, Fagus hayatae Palibin ex Hayata."New Forests. 19. 109-114 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Mikami: "Molecular analysis of cytoplasmic male sterility in beets."Acta Horticulturae. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 三上哲夫: "花-性と生殖の分子生物学(日向康吉 編)"学会出版センター(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 三上哲夫: "栽培植物の自然史(山口裕文 編)"北海道大学図書刊行会(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] S. Kato: "Analysis of mitochondrial DNA of an endangered beech species, Fagus hayatae Palibin ex Hayata"New Forests. 19. 109-114 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Kubo: "Alterations in organization and transcription of the mitochondrial genomes of cytoplasmic male sterile sugar beet (Beta vulgaris L..)"Molecular and General Genetics. 262. 283-290 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Onodera: "Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet"Journal of Plant Physiology. 155. 656-660 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Yamashita: "Resistance to gap repair of the transposon Tam3 in Antirrhinum majus ; a role of the end regions"Genetics. 153. 1899-1908 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Nishizawa: "Variable number of tandem repeat loci in the mitochondrial genomes of beets"Current Genetics. 37. 34-38 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Kubo: "The nad4L-orf25 gene cluster is conserved and expressed in sugar beet mitochondria"Theoretical and Applied Genetics. 100. 214-220 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三上 哲夫: "花 ― 性と生殖の分子生物学 (日向 康吉 編)"学会出版センター (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] 三上 哲夫: "栽培植物の自然史 (山口 裕文 編)"北海道大学図書刊行会 (印刷中).

    • Related Report
      1999 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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