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THE ANALYSIS ON MOLECULAR MECHANISM OF INECTION ORGANIC DIFFERENTIATION IN PLANT PATHOGENIC FUNGI

Research Project

Project/Area Number 11460150
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物資源科学
Research InstitutionIBARAKI UNIVERSITY

Principal Investigator

AKUTSU Katusmi  IBARAKI Univ., FACULTY OF AGRICULTURE, PROFESSOR, 農学部, 教授 (10151002)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Masami  IBARAKI Univ., FACULTY OF AGRICULTURE, ASSISTANT PROFESSOR, 農学部, 助手 (70301075)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1999: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsBotrytis cinerea / multicellular appressorium / pathogenicity / inosine / GTP-binding protein / differntial display / aspartic proteinase / gene disruption / Botrytis fabae / Cutinase 遺伝子 / Polygalacturnase遺伝子 / 侵入器官 / クローニング / 遺伝子構造 / 侵入器官分化 / サイクリックAMP / 多犯性病原菌 / 膜タンパク質 / 分子スイッチ
Research Abstract

Action mechanisms of inosine and cyclicAMP were analyzed in order to clarify the differentiation mechanism of multicellular appressoria, the infection organ of gray mold fungus (Botrytis cinerea). The inosine induced the formation of the appressoria in the some starins, and promoted infection to cucumber plants. Though the induction effect by the cAMP was lower than the inosine, its effect increased with the progress in the time. The inosine was suggested to be an inducer of the appressorium differentiation, because cyclicAMP was metabolized into inosine in the fungal mycelia. By the combination of SDS-PAGE and interaction analyzer (Iasys), 19kD protein, which peculiarly combined with the inosine, was got in the fractionation of the fungal cell membrane protein. In the N-terminal sequence, this protein showed the high homology for the amino acid sequence of the GTP-binding protein.
Next, the isolation of the gene, which peculiarly appeared in the differentiation of the multicultural appresoria, was tried by Differential Display method. The result showed that there was a difference between both samples in the band pattern of PCR amplified product with the polyacrylamide electrophoresis, when several primer was used. The base sequences of the bands peculiarly detected in the appressorium differentiation were decided. The amino acid sequence of which the one band was anticipated showed the high homology for the arrangement of aspartic proteinase. Aspartic proteinase is known as an enzyme peculiarly secreted in the infection in opportunistic infection fungi such as Aspergillus fumigatus and Candida albicans, and it is suggested to be deeply concerned in the pathogenicity. At present, the roles in the formation of the appressorium and the infection are analyzed by disruption of the aspartic proteinase gene.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Lee, Y.J. et al.: "Distribution of the Consensus Sequence of ABC Transporter Gene among Several Taxonomically Distinct Phtopathogenic Fungi"JGPP. 67・2. 106-110 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, M. et al.: "Functional Analysis of an ATP-Binding Cassette Transporter Gene in Botrytis cinerea by Gene Disruption"JGPP. 67・3. 212-214 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kishimoto, K. et al.: "Detailed analysis of rice chitinase gene expression in transgenic cucumber plants showing different levels of disease resistance to gray mold (Botrytis cinerea)"Plant Science. 162(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 吉田 愛: "Botrytis cinereaの付着器形成機構-イノシン結合タンパクについて-"日本植物病理学会報. 64・4. 347 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 喜多 真幸: "Botrytis cinereaの付着器形成に関与する遺伝子の探索"日本植物病理学会報. 64・4. 368 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 喜多 真幸: "Botrytis fabseのクチナーゼおよびポリガラクツロナーゼ遺伝子の単離"日本植物病理学会報. 66・3. 124-125 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, M. et al.: "Functional Analysis of ATP-Binding Cassette Transporter Gene in Botrytis cinerea by Gene Disruption"Proceedings of The First Asian Conference on Plant Pathology. 24 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 鈴木 淳子: "灰色かび病菌(Botrytis cinrera)のABCトランスポーター遺伝子BMR1の機能解析"日本植物病理学会報. 67・2. 217-218 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Lee, Y.J., Hamamoto, H., Nakaune, R., Nawata, O., Makizumi, Y., Hibi, T. and Akutsu, K.: "Distribution of the Consensus Sequence of ABC Transporter Gene among Several Taxonomicall Distinct Phytopathogenic Fungi"JGPP. 67-2. 106-110 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, M., Suzuki, J., Hosaka, T., Hibi, T. and Akutsu, K.: "Functional Analysis of ATP-Binding Cassette Transporter Gene in Botrytis cinerea by Gene Disruption"JGPP. 67-3. 212-214 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kishimoto, K., Nishizawa, Y, Tabei, Y, Hibi, T., Nakajima, M. and Akutsu, K.: "Detailed analysis of rice chitinase gene expression in transgenic cucumber plants showing different levels of disease resistance to gray mold (Botrytis cinerea)"Plant Science. 162 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshida, A., Nakao, Y., Nkajima, M. and Akutsu, K.: "Mechanisms of the appressorium formation of Botrytis cinerea-Inosine-binding protein"Ann. Phytopathol. Soc. Jpn.. 64-4. 347 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kita, M., Hosaka, T., Nakajima, M. and Akutsu, K.: "The search of the gene concerning the appressorium formation in Botrytis cinerea"Ann. Phytopathol. Soc. Jpn.. 64-4. 368 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kita, M., Hosaka, T., Nakajima, M. and Akutsu, K.: "The isolation of polygalacturonase gene and cutinase in, Botrytis fabse"Ann. Phytopathol. Soc. Jpn.. 66-2. 124-125 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, M., Suzuki, J., Hosaka, T., Hibi, T. and Akutsu, K.: "Functional Analysis of ATP-Binding Cassette Transporter Gene in Botrytis cinerea by Gene Disruption"Proceedings of The First Asian Conference on Plant Pathology. 24. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, J., Hosaka, T., Nakajima, M., Hibi, T. and Akutsu, K.: "The. functional analysis of ABC transporter gene BMR1 in gray mold fungus (Botrytis cinerea)"Ann. Phytopathol. Soc. Jpn.. 67-4. 217-218 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, M. et al.: "Functional Analysis of an ATP-Binding Cassette Transporter Gene in Botrytis cinerea by Gene Disruption"JGPP. 67・3. 212-214 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 鈴木 淳子等: "灰色かび病菌(Botrytis cinrera)のABCトランスポーター遺伝子BMRIの機能解析"日本植物病理学会報. 67・2. 217-218 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 井上 久美子等: "灰色かび病菌(Botrytis cinerea)における多細胞型付着器形成に関与する遺伝子の単離"平成14年度日本植物病理学会大会. (講演予定).

    • Related Report
      2001 Annual Research Report
  • [Publications] 深井 直之等: "灰色かび病菌の付着器形成時に特異的に発現するタンパク質の二次元電気泳動による解析"平成14年度日本植物病理学会大会. (講演予定).

    • Related Report
      2001 Annual Research Report
  • [Publications] 喜多真幸ら: "Botrytis fabaeからのクチナーゼおよびポリガラクツロナーゼ遺伝子の単離"日本植物病理学会報. 66・2. 124-125 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 中畝良二ら: "カンキツ緑かび病菌Penicillium digitatumの薬剤耐性と病原性の関与するABCトランスポーター遺伝子PMR5"日本植物病理学会報. 66・2. 130 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakajima,M. et al.: "Functional Analysis of ATP-Binding Cassette Transporter Gene in Botrytis cinerea by The Gene Disruption"Proceedings of The First Asian Conference on Plant Patholog. 24 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鈴木淳子: "灰色かび病菌Botrytis cinereaのABCトランスポーター遺伝子BMR1の機能解析"平成13年度日本植物病理学会大会講演予定.

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉田 愛ら: "Botrytis cinerea の付着器形成機構-イノシン結合タンパクについて-"日本植物病理学会報. 64・4. 347 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 喜多 真幸ら: "Botrytis cinerea の付着器形成に関与する遺伝子の探索"日本植物病理学会報. 64・4. 368 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 喜多 真幸ら: "Botrytis fabae からのクチナーゼおよびポリガラクツロナーゼ遺伝子の単離"日本植物病理学会大会(岡山大学). (発表予定). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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