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ヒト癌におけるP16-COK-Rb細胞回転異常に関する病理学的研究

Research Project

Project/Area Number 11470049
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionGunma University

Principal Investigator

NAKAJIMA Takashi  Gunma University, Professor, 医学部, 教授 (20124422)

Co-Investigator(Kenkyū-buntansha) SANO Takaaki  Gunma University, Assistant professor, 医学部, 講師 (90292581)
KASHIWABARA Kenji  Yamanashi Medical School, Associate professor, 附属病院, 助教授 (80242634)
OYAMA Tetsunari  Gunma University, Associate professor, 医学部, 助教授 (50233622)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsHuman cancers / Cell cycle regulator proteins / RB pathway / p53 pathway / Immunohistochemistry / Rb経路 / 細胞回転 / p16蛋白 / CDK / pRb蛋白 / p53蛋白
Research Abstract

Expression of cell cycle regulatory proteins in both the RB and p53 pathways were investigated in lung, esophageal and oral cancers using immunohisto-chemical techniques. In squamous cell carcinoma (SCC) of lung, abnormality of p16 expression was prominent at the frequency of 78% in the RB pathway. On the other hand, strong and diffuse p53 immunoreactivity was seen in 60% of SCCs, which seemed to have point mutation of the p53 gene. In comparing clinicopathological status with the immunohistochemical results, strong p53 expression was frequently observed in higher stages of SCC, with the developing tumor located in the central field of the lung. Similarly, the frequency of p14ARF expression was high in centrally developed SCC.
In stage I adenocarcinoma of the lung, abnormality of the RB pathway was not frequent. However, abnormal expression of p14ARF and MDM2 was characteristic features in the p53 pathway. Overexpression of MDM2 protein was characteristic feature in stage I adenocarcinomas, especially in well differentiated ones. These findings elucidate that cell cycle abnormality is mostly depend on p53 pathway other than RB pathway in stage I adenocarcinomas of lung.
The abnormality of both RB and p53 pathways was also observed in esophageal and oral squamous cell carcinomas. Therefore, abnormality in RB and p53 pathways was essential for development of human cancers.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Xue,Qi: "Aberrant expression of pRb, p16, p14ARF, MDM2, p21 and p53 in squamous cell carcinoma of lung."Jpn J Cancer Res. March(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sano,T.: "Immunchistochemical inactivation of p14ARF concomitant with MDM2 over-Expression inversely correlated with p53 overexpression in oral squamous cell carcinoma."Pathology Int. 50. 709-716 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato,H.: "An immunchistochemical study of p16, pRb, p21 and p53 proteins in human esophageal cancers."Anticancer Res. 20. 345-350 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Niwa,Y.: "BRCA1 expression status in relation to DNA methylation of the BRCA1 promoter region in sporadic breast cancers."Jpn J Cancer Res. 91. 519-526 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishihara,S.: "The cyclin-dependent kinase inhibitor p27 as a prognostic factor in advanced non-small cell lung cancer ; its immunchistochamical evaluation using biopsy specimens."Lung Cancer. 26. 187-194 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saito,T.: "Immunchistochemical analysis of cell cycle-associated proteins p16, pRb, p53, p27 and Ki-67 in oral cancer and precancer with special reference to verrucous carcinoma."J Oral Pathol Med. 28. 226-232 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xue, Q., Sano, T., Kashiwabara, K., Oyama, T.and Nakajima, T.: "Aberrant expression of pRb, p16, p14ARF, MDM2, p21 and p53 in squamous cell carcinoma of lung."Jpn.J.Cancer Res.. (March, in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sano, T., Hikino, T., Xue, Q., Saito, T., Kashiwabara, K., Oyama, T.And Nakajima, T.: "Immunohistochemical inactivation of p14ARF concomitant with MDM2 overexpression inversely correlates with p53 overexpression in oral squamous cell carcinoma."Pathology Int.. 50. 709-716 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato, H., Yoshikawa, M., Fukai, Y., Tajima, K., Masuda, N., Tsukada, K., Kuwano, H.and Nakajima, T.: "An immunohistochemical study of p16, pRb, p21 and p53 proteins in human esophageal cancers."Anticancer Res.. 20. 345-350 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato, H., Miyazaki, T., Yoshikawa, M., Nakajima, M., Fukai, Y., Tajima, K., Masuda, N., Tsutsumi, S., Tsukada, K., Nakajima, T.and Kuwano, H.: "Nitrotyrosine in esophageal squamous cell carcinoma and relevance to p53 expression."Cancer Letters. 153. 121-127 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Niwa, Y., Oyama, T.and Nakajima, T.: "BRCA1 expression status in relation to DNA methylation of the BRCA1 promoter region in sporadic breast cancers."Jpn.J Cancer Res.. 91. 519-526 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishihara, S., Minato, K., Hoshino, H., Saito, R., Hara, F., Nakajima, T.and Mori, M.: "The cyclin-dependent kinase inhibitor p27 as a pprognostic factor in advanced non-small-cell lung cancer : its immunohistochemical evaluation using biopsy specimens.."Lung Cancer. 26. 187-194 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saito, T.Nakajima, T.and Mogi, K.: "Immunohistochemical analysis of cell cycle-associated proteins p16, pRb, p53, p27 and Ki-67 in oral cancer and precancer with special reference to verrucous carcinoma"J Oral Pathol Med.. 28. 226-232 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xue,Q. et al.: "Aberrant expression of pRb, p16, p14ARF, MDM2 p21 and p53 in squamous cell carcinoma of lung."Jpn J Cancer Res. March (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sano,T. et al.: "Immunohistochemical inactivation of p14ARF concomitant with MDM2 overexpression inversely correlate with---."Pathol Int. 50. 709-716 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kato,H. et al.: "An immunohistochemical study of p16, pRb, p21 and p53 proteins in human esophageal cancers."Anticancer Res.. 20. 345-350 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Niwa,Y. et al.: "BRCA1 expression status in relation to DNA methylation of the BRCA1 promoter region in sporadic breast cancers."Jpn J Cancer Res. 91. 519-526 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishihara,S. et al.: "The cyclin-dependent kinase inhibitor p27 as a prognostic factor in advanced non-small cell lung cancer :"Lung Cancer. 26. 187-194 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Saito,T. et al.: "Immunohistochemical analysis of cell cycle-associated proteins p16,pRb,p53,p27 and Ki-67 in oral cancer---."J Oral Pathol Med. 28. 226-232 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Saito T,Nakajima T&Mogi K: "Immunohistochemical analysis of dell cycle-associated proteins p16,pRb,p53,p27andKi67 in oral oanoor and procancer with special reference to verrucous carcinoma"J Oral Pathol Med. 28. 226-232 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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