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Role of Epstein-Barr virus-encoded latent membrane protein 2A on maintenance of latent infection

Research Project

Project/Area Number 11470073
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionHokkaido University

Principal Investigator

TAKADA Kenzo  Hokkaido Univ., Institute for Genetic Medicine, Prof., 遺伝子病制御研究所, 教授 (30133721)

Co-Investigator(Kenkyū-buntansha) MARUO Seiji  Hokkaido Univ., Institute for Genetic Medicine, Inst., 遺伝子病制御研究所, 助手 (70292018)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2000: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1999: ¥9,500,000 (Direct Cost: ¥9,500,000)
KeywordsEpstein-Barr virus / LMP2A / latent infection / membrane immunoglobulin / 活性化 / カルシウム動員
Research Abstract

To quantitatively evaluate the role of Epstein-Barr virus (EBV)-encoded latent membrane protein 2A(LMP2A) in immortalization of peripheral B-lymphocytes, we used the Akata cell system to generate an EBV recombinant in which the first exon of the LMP2A gene was disrupted. The results indicated that deletion of the LMP2A gene did not affect the immortalization efficiency of EBV in B-lymphocytes. Deletion of the LMP2A gene made EBV-transformed lymphocytes more permissive for virus replication in response to surface immunoglobulin cross-linking. On the other hand Akata cells, in which LMP2A expression was much lower than in EBV-transformed lymphocytes, were equally permissive for virus replication whether they were infected with wild EBV or LMP2A-knockout EBV. The results raise a question as to the role of LMP2A in inhibition of disruption of virus latency in vivo, where LMP2A expression has been expected to be low as in Akata cells. Furthermore, to clarify the strain variation of the LMP2A gene, we determined the nucleotide sequence of the LMP2A gene of Akata strain and compared it with that of B95-8 strain. Three nucleotide differences were found between Akata and B95-8 strains, which resulted in three amino acid changes, but the two ITAM motifs that were important for blocking signal transduction through crosslinking of B-cell surface immunoglobulins were conserved in two EBV strains. These results suggest that the LMP2A genes of both viruses are not functionally different.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Konishi, K.: "Role of Epstein-Barr virus-encoded latent membrane protein 2A on virus-induced I I immortalization and virus activation"J Gen. Virol.. 82. 1451-1456 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitagawa, N.: "Epstein-Barr virus-encoded poly-A RNA supports Burkitt's lymphoma growth through IL-10 induction"EMBO J.. 19. 6742-6750 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Komano, J.: "Role of bcl-2 in Epstein-Barr-induced malignant conversion of Burkitt's lymphoma cell line Akata"J. Virol.. 75. 1561-1564 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang, L.: "CD21-mediated entry and stable infection by Epstein-Barr virus in canine and rat cells"J. Virol.. 74. 10745-10751 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oda, T.: "Epstein-Barr virus lacking glycoprotein gp85 can not infect B cells and epithelial cells"Virology. 276. 52-58 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeda, T: "Lytic Epstein-Barr virus infection in the synovial tissue of rheumatoid arthritis"Arthritis Rheum.. 43. 1218-1225 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Konishi, K.: "Role of Epstein-Barr virus-encoded latent membrane protein 2A on virus-induced immortalization and virus activation"J. Gen. Virol.. 82. 1451-1456 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitagawa, N.: "Epstein-Barr virus-encoded poly-A- RNA supports Burkitt's lymphoma growth through IL-10 induction"EMBO J.. 19. 6742-6750 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Komano, J.: "Role of bcl-2 in Epstein-Barr-induced malignant conversion of Burkitt's lymphoma cell line Akata"J. Virol.. 75. 1561-1564 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang, L.: "CD21-mediated entry and stable infection by Epstein-Barr virus in canine and rat cells"J. Virol.. 74. 10745-10751 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oda, T.: "Epstein-Barr virus lacking glycoprotein gp85 can not infect B cells and epithelial cells"Virology. 276. 52-58 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeda, T.: "Lytic Epstein-Barr virus infection in the synovial tissue of rheumatoid arthritis"Arthiritis Rheum.. 43. 1218-1225 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Konishi, K.: "Role of Epstein-Barr virus-encoded latent membrane protein 2A on virus-induced immortalization and virus activation."J Gen.Virol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitagawa, N.: "Epstein-Barr virus-encoded poly-A^- RNA supports Burkitt's lymphoma growth through IL-10 induction."EMBO J.. 19. 6742-6750 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Komano, J.: "Role of bcl-2 in Epstein-Barr-induced malignant conversion of Burkitt's lymphoma cell line Akata."J.Virol.. 75. 1561-1564 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yang, L.: "CD21-mediated entry and stable infection by Epstein-Barr virus in canine and rat cells."J.Virol.. 74. 10745-10751 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Oda, T.: "Epstein-Barr virus lacking glycoprotein gp85 can not infect B cells and epithelial cells."Virology. 276. 52-58 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeda, T: "Lytic Epstein-Barr virus infection in the synovial tissue of rheumatoid arthritis."Arthritis Rheum.. 43. 1218-1225 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Komano, J.: "Oncogenic role of Epstein-Barr virus-encoded RNAs (EBERs)in Burkitt's lymphoma cell line Akata"J. Virol.. 73. 9827-9831 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishikawa, J.: "EBV promotes epithelial cell growth in the absence of EBNA2 and LMP1 expression"J. Virol.. 73. 1286-1292 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugawara, Y.: "Enhancement of hepatitis C virus replication by Epstein-Barr virus-encoded nuclear antigen"ENBO J.. 18. 5755-5760 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugawara, Y.: "Detection of EBV in hepatocellular carcinoma tissue : a novel EBV latency characterized by absence of EBER expression"Virology.. 256. 196-202 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takada, K.: "Epstein-Barr virus and gastric carcinoma (Review)"Epstein-Barr Virus Report. 6. 95-100 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugawara, Y.: "Detection of hepatitis B virus (HBV)DNA in the tissues of hepatocellular carcinomas related to hepatitis C virus that are negative for HBV surface antigen"Scand.J.Gastroenterol.. 34. 934-938 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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