| Project/Area Number |
11470100
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
YAMAUCHI Hiroshi St.Marianna University School of Medicine, Dept.of Preventive Medicine, Associate professor, 医学部, 助教授 (90081661)
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| Co-Investigator(Kenkyū-buntansha) |
AMINAKA Masahito St.Marianna University School of Medicine, Dept.of Preventive Medicine, Assistant, 医学部, 助手 (30231997)
ARAI Fumio St.Marianna University School of Medicine, Dept.of Preventive Medicine, Assistant, 医学部, 助手 (90103481)
YOSHIDA Katsumi St.Marianna University School of Medicine, Dept.of Preventive Medicine, professor, 医学部, 教授 (80158435)
NAKAI Izumi Science University of Tokyo Dept.of Applied Chemistry, Professor, 理学部, 教授 (90155648)
斉藤 秀 聖マリアンナ医科大学, 医学部, 助手 (90308445)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | acute arsenic poisoning / embryo / newbaby / brain damage / DNA damage / apoptosis / methylation / behavioristics |
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| Research Abstract |
We investigated the relationship between acute exposure to arsenic trioxide during the fetal period and the occurrence of brain damage in the fetus and childhood. We gave a single oral administration of arsenic trioxide to pregnant rats at the 17th day of gestation and observed the changes in arsenic concentration in the brains of the fetuses and mother rats at 12 to 48 hr after administration. Compared with the control, a marked rise in arsenic concentration was recognized in the exposed group fetuses. Arsenic concentration in the fetal brains (inorganic arsenic and its metabolites) was three times higher than that of the control at 12 to 48 hr after administration. The dynamics of the chemical specis of the arsenic detected in the fetal brains were : concentration of inorganic arsenic was rising at 12 hr after administration, but at every time point, what was rising mainly was dimethylated arsenic (DMA), the final metabolite of arsenic trioxide, in the fetal brains. Histopathological examination was carried out on the mother rats and fetuses. No apoptosis or necrosis was recognized in the brains of the mother rats. Neither was necrosis recognized in the fetal brains. In contrast to this, appearance of apoptotic cells was clearly recognized in the fetal brains at 12, 24 and 48 hr after administration of arsenic trioxide. Detection of apoptotic cells in the fetal brains was most marked in the 12 hr group. The results of this study show clear brain tissue damage in cases of exposure to relatively high doses of inorganic arsenic in the fetal period, and this suggests possible future brain damage as squeal.
We thought that the generation of the cerebral disorder by the arsenic exposure of the embryo period was suggested in a behavioristics inspection result of the organization diagnosis, and would have the necessity which was the enough development by the research of this field in the future in this research.
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