| Project/Area Number |
11470105
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
YAMAGATA Zentaro Tamanashi Medical Universi ty, Heal th Sciencea Professor, 医学部, 教授 (10210337)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Alzheimer's disease / gene polymorphisms / APOE / VLDL / Life style |
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| Research Abstract |
We evaluated that an association between putative risk factors and Alzheimer's disease (AD) according gene p olym orp hisms.
The subjects were 407 patients (168 men and 239 women :age at onset 69±10) with a diagnosis of probable AD based on the NINCDS-ADRDA criteria. Three hundred and ninety eight spouses (162 men and 236 women : age 69±9) of the subjects served as control. The risk factor interview was developed specifically for this study to assess 17 putative risk factors. APOE gene polymorphisms were determined by the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. The triplet (CGG) repeat polymorphism of very low density lipoprotein receptor gene (VLDLr) was genotyped by the PCR fragment analysis software of Long Read Tower sequencer. The estimating odds ratio and the multiple logistic analysis were performed by SAS statistic analysis package to evaluate the association. This study was conducted after obtaining written informed consent from the subjects or their guardians.
The genetic analysis revealed the AD was associated with VLDLr as well as APOE : he odds ratio was 2.98 for the e4 allele. The frequency of the 5-repeat ; allele ofVLDLr was significantly higher in AD than in control(p<0.04). The odds ratio for 5-repeat allele was 1.56 (95 % CI 1.32-1.78). Some of putative risk factors including aging, family history of AD, education years, napping and death of partner were confirmed as risk factors for AD. Though most of these risk factors were not interactive with gene polymorphisms, onset age was associated with APOE but not VLDLr. And limited habitual napping for up to 60 min had a protective effect against the development of AD especially for carriers of the APOE e4 (OR=0.49, 95 % CI :0.33-0.73).
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