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Physiological and Biochemical Studies on apelin, a novel gastrointestinal hormone from stomach

Research Project

Project/Area Number 11470127
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionGunma University

Principal Investigator

TATEMOTO Kazuhiko  Institute for Molecular and Cellular Regulation, Gunma University, Professor, 生体調節研究所, 教授 (60240694)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Isao  Faculty of Medicine, Gunma University, Professor, 医学部, 教授 (50008273)
TAKAYAMA Kiyoshige  Faculty of Health Sciences, Gunma University, Professor, 医学部, 教授 (90134270)
SHIMIZU Hiroyuki  Faculty of Medicine, Gunma University, Assistant Professor, 医学部, 講師 (20251100)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1999: ¥8,400,000 (Direct Cost: ¥8,400,000)
Keywordsapelin / APJ / cholecystokinin / gastrointestinal hormone / gastric mucosa / coreceptor / HIV / food intake / オーフォン受容体 / 生体防御機構 / 一酸化窒素 / 摂食調節 / オーファン受容体 / ラジオイムノアッセイ / NO合成阻害剤
Research Abstract

The principal aim of this proposal is to study physiological and biochemical characterization of apelin, a novel gastrointestinal hormone isolated from the stomach. Toward this goal, a specific immunoassay for apelin has been developed, and using this assay, we found that apelin is distributed in a wide variety of tissues including the spleen, liver, mammary gland, pancreas, kidney, spinal cord, lung, stomach, brain, intestine, and heart. We also determined the molecular forms of apelin in these tissues. In the lung, kidney, intestine, and brain, a major peak of apelin was observed at a position almost identical to that of apelin-17, while, in the spleen, a major peak was detected at a position corresponding to that of apelin-36. The liver extract seemed to contain two major peptides similar to apelin-13 and apelin-17. We also studied the location of apelin in rat tissues using immunohistochemical techniques. Apelin was present in the endothelia of the small arteries of the spleen, lung, intestine, kidney, heart, pancreas, liver, and gastrointestinal tract. Apelin was also found in the white adipose tissues and the stomach wall. We examined biological activities of apelin and found that apelin lowered blood pressure in rats and also released cholecystokinin (CCK) from dispersed intestinal endocrine cells. We also found that apelin is present in milk, thus, the peptide may have a role as an exogenous CCK-releasing factor. Since apelin is a ligand for the HIV coreceptor APJ, we tested the effect of apelin on HIV infection, and found that apelin blocked the entry of HIV-1 and HIV-2. Apelin-36 more effectively blocked HIV infection than apelin-12 or apelin-13. We also found that the administration of apelin suppresses cytokine production by mouse spleen cells. These studies support the speculation that apelin may be involved in the modulation of immune responses.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Habata, Y.: "Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum"Biochem. Biophys. Acta. 1452. 25-35 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kogure, K.: "Evaluation of serum uric acid changes in different forms of hepatic vascular inflow occlusion in human liver surgeries"Life Sciences. 64. 305-313 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Zou, M-X.: "Apelin peptides block the entry of human immunodeficiency virus (HIV)"FEBS Letters. 473. 15-18 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 立元 一彦: "オーファン受容体を利用した新規生理活性物質の検索"日本臨床. 58. 737-746 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatemoto, K.: "Apelin : A novel regulatory peptide discovered as an endogenous ligand for orphan G protein-coupled receptor APJ"Regulatory Peptides. 94. 4-4 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatemoto, K.: "The novel peptide apelin lowers blood pressure via a nitric oxide dependent mechanism"Regulatory Peptides. 99. 87-92 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Habata Y: "Apelin, the natural ligand of the orphan receptor APJ, is secreted in the colostrum"Biochem. Biophys Acta. 452. 25-35 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kogure K: "Evaluation of serum uric acid changes in different forms of hepatic vascular inflow occlusion in human liver surgeries"Life Sciences. 64. 305-313 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatemoto K: "Apelin : A novel regulatory peptide discovered as an endogenous ligand for orphan G protein-coupled receptor APJ"Regulatory Peptides. 94. 4-4 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatemoto K: "Search for an endogenous ligand of the orphan G protein-coupled receptor"Nippon Rinsho. 58. 737-746 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Zou M-X: "Apelin peptides block the entry of human immunodeficiency virus (HIV)"FEBS Letters. 473. 15-18 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatemoto K: "The novel peptide apelin lowers blood pressure via a nitric oxide dependent mechanism"Regulatory Peptides. 99. 87-92 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Habata,Y.: "Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum."Biochem.Biophys.Acta. 1452. 25-35 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kogure,K.: "Evaluation of serum uric acid changes in different forms of hepatic vascular inflow occlusion in human liver surgeries."Life Sci.. 64. 305-313 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 立元一彦: "オーファン受容体を利用した新規生理活性物質の検索"日本臨床. 58. 737-746 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zou,M-X.: "Apelin peptides block the entry of human immunodeficiency virus (HIV)."FEBS Letters. 473. 15-18 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tatemoto,K.: "Apelin : A novel regulatory peptide discovered as an endogenous ligand for orphan G protein-coupled receptor APJ."Regulatory Peptides. 94. 4-4 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tatemoto,K.: "The novel peptide apelin lowers blood pressure via a nitric oxide dependent mechanism."Regulatory Peptides. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Habata,Y.: "Apelin,the natural ligand of the orphan receptor APJ,is abundantly secreted in the colostrum"Biochem.Biophys.Acta. 1452. 25-35 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kogure,K..: "Evaluation of serum uric acid changes in different forms of hepatic vascuar infow occlusion in human liver surgeries"Life Sci.. 64. 305-313 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 立元一彦: "オーファン受容体を利用した新規生理活性物質の検索"日本臨床. 58. 746-737 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zou,M-X.: "Apelin peptides block the entry of human immunodeficiency virus(HIV)"FEBS Letters. (in press).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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