| Project/Area Number |
11470141
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | KUMAMOTO UNIVERSITY |
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Principal Investigator |
ANDO Masayuki Kumamoto University, School of Medicine, 1st Department of Internal medicine, Professor, 医学部, 教授 (00040204)
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| Co-Investigator(Kenkyū-buntansha) |
SUGA Moritaka Kumamoto Univ.Sch of Med, Associate Professor, 医学部, 助教授 (20154437)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | summer-type hypersensitivity pneumonitis / Trichosporon asahii / T.mucoides / molecular biology / Trichosporon mucoides / Cryptococcus neoformans / BAL / Trichosporon T.asahii / Trichosporon T.mucoides |
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| Research Abstract |
We assigned the causative agents of summer-type hypersensitivity pneumonitis(SHP) as Trichosporon asahii and T.mucoides, using molecular data. In this study, we applied polymerase chain reaction(PCR) and Southern hybridization to detect the antigen specific DNAs in the bronchoalveolar lavage fluid(BALF) of a murine model or patients with SHP, and in the air of patients' homes. BALF samples of the model were positive in 100% and 60% at 6h and 96h after the inhalation challenge, respectively. The BALF samples of 18 patients obtained within a week after admission were positive in 94%. Those of 23 patients with other diseases and 7 healthy volunteers were 22% and 29%, respectively. Air samples were collected by Andersen type low volume air sampler. Particles on the sampling plates were harvested by ultrasonic cleaner and amplified by PCR.The 2/2 air samples were positive. The each signal of T.asahii and T.mucoides were discriminated from the other fungi or human cells by PCR itself of additional restriction enzyme, Sau3AI.The detection of pathogen specific DNAs in host and environment by PCR would be an expandable method to investigate the pathogenesis of occupational diseases caused by microbial exposures.
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