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ANALYSIS OF MECHANISMS OF DECREASED CERAMIDE PRODUCTION IN CORNEUM STRATUM PATIENTS WITH ATOPIC DERMATITIS

Research Project

Project/Area Number 11470168
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionGUNMA UNIVERSITY

Principal Investigator

MORIKAWA Akihiro  DEPARTMENT OF PEDIATRICS, GUNMA UNIVERSITY SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (40125878)

Co-Investigator(Kenkyū-buntansha) ARAKAWA Hirokazu  DEPARTMENT OF PEDIATRICS, GUNMA UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (50272232)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsatopic dermatitis / barrier function / TEWL / ceramide / sphingomyelinase / microsatellite / polymorphism / atopic gene / スフインゴミエリナーゼ / アトピー候補遺伝子 / 角質層 / セラミド合成酵素 / 特異lgE
Research Abstract

1) Impaired barrier function may contribute to the pathophysiology of atopic dermatitis. Ceramides are the main polar lipids of the stratum corneum and play an important role in skin barrier function, cell adhesion and epidermal differentiation. The transepidermal water loss (TEWL), serum IgE and eosinophils count in the peripheral blood were measured in children with atopic dermatitis. The level of TEWL was significantly higher in children with atopic dermatitis than those with bronchial asthma or controls. Neither the level of serum IgE nor eosinophils count were associated with the level of TEWL, suggesting that impaired barrier function is independent of atopic predisposition.
2) To elucidate the mechanisms that are involved in the decrease of ceramide levels in atopic dry ski, we evaluated the possible role of the gene for sphingomyelinase, which is a major enzyme of ceramide production, in modulating atopic dermatitis in the Japanese population. We screened all 6 exons of the sphingomyelinase gene from 5 subjects for mutations by direct polymerase chain reaction (PCR) sequencing. The sphingomyelinase gene polymorphisms were genotyped by PCR fragment length polymorphism analysis. We also evaluated the gene polymorphism for the IL-4 receptor Q576R and STAT6, which is a key transcription factor involved in both IL-4 and IL-13-mediated biological responses. We found a novel dinucleotide repeat polymorphism in the first exon of the sphingomyelinase gene. The genotypes were classified into 6 groups according to the number of hexamer repeats present, from 5 to 11. The frequency of the 7 repeat homozygote was higher in children with atopic dermatitis than controls. This repeat polymorphism was not associated with the polymorphism in the IL-4 receptor (Q576R) and stat6. This suggests that genetic variation in the sphingomyelinase may be associated with predisposition to atopic dermatitis. In addition, this polymorphism may be independent of atopic gene.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Takizawa-T, Arakawa-H et al: "Identification of allergen factions of wheat flour responsible for anaphylactic reactions to wheat products in infants and young children."Int Arch Allergy Immunol. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arakawa-H.et al: "Airway responsibless to acetylchdine or capsaicin in immature and mature guinea pigs in vivo"Allergology International. 49. 99-104 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mochizuki-H et al: "Bronchial hyperresponsiveness before and after the diagnosis of bronchial asthma ion children"Pediatrics. 106. 1442-1446 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mochizuki-H et al: "Effect of ultrasonically rebulized distilled water on airway epithelial cell swelling in guinea pigs"J.Appl Physiol. 86. 1505-1512 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakashima-K. et al: "Astrocyte differentiation mediated by LIF in cooperation with BMPZ"FEBS Letters. 457. 43-46 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arakawa-H et al: "Efficacy of inhaled antichdinergics and anesthesia in treatment of a patient in status asthmatics"J.Asthma. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takizawa T, Arakawa H, Tokuyama K, Morikawa A.: "Identification of allergen fractions of wheat flour in infant and young children with anaphylaxis to wheat products."Int Arch Allergy Immunol. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arakawa H, Tokuyama K, Morikawa A, Lovall J.: "Non-cholinergic neural bronchoconstriction induced by i.v. capsaicine are comparable in immature and adult guinea pigs."Internatinal Allergology. 49. 99-104 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mochizuki H, Ohki Y, Arakawa H, Tokuyama K, Morikawa A.: "Effect of ultrasonically nebulized distilled water on airway epithelial cell swelling in guinea pigs."J Appl Physiol. 86. 1505-1512 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mochizuki H, Ohki Y, Arakawa H, Tokuyama K, Morikawa A.: "Effect of ultrasonically nebulized distilled water on airway epithelial cell swelling in guinea pigs."J Appl Physiol. 86. 1502-1512 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakashima K, Yanagisawa M, Arakawa H, Taga T.: "Astrocyte differentiation mediated by LIF in cooperation with BMP2."FEBS Letters. 457. 43-46 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yanagisawa M, Nakashima K, Takizawa T, Ochiai W, Arakawa H, Taga T.: "Signaling crosstalk underlying synergistic induction of astrocyte differenciation by BMPs and IL-6 family of cytokines."FEBS Lett.. 489. 139-143 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arakawa H, Takizawa T, Tokuyama K, Mochizuki H, Morikawa A.: "Efficacy of inhaled anticholinergics and anesthesia in treatment of a patient in status asthmaticus."J Asthma. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takizawa-T,Arakawa-H et al: "Idintification of allergen factions of wheat flour responsible for anaplylactic reactions to wheat products in infants and young Children."Int Arch Allergy Immunol. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Arakawa-H. et al: "Airway responsibless to acetylchdine or capsaicin in immature and mature guinea pigs in vivo"Allergology International. 49. 99-104 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mofchizuki-H et al: "Bronchial hyperresponsiveness before and after the diagnosis of bronchial asthma in children"Pediatrics. 106. 1442-1446 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mochizuki-H et al: "Effect of ultrasonically rebulized distilled water on airway epithelial cell swelling in guinea pigs"J.Appl Physiol. 86. 1505-1512 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakashima-K: "Actrocyte differentiation mediated by CIF in cooperation with BMP2"FEBS Letters. 457. 43-46 (1999)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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