| Project/Area Number |
11470242
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Shinshu University |
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Principal Investigator |
HASHIKURA Yasuhiko Shinshu University Hospital, Lecturer, 医学部・附属病院, 講師 (10228398)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAZAWA Yuichi Shinshu University, Assistant professor, 医学部, 助手 (10273088)
KAWASAKI Seiji Shinshu University, Professor, 医学部, 教授 (80177667)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | Fas / Fas ligand / apoptosis / Fasligand |
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| Research Abstract |
We investigated the role of the apoptosis through Fas/Fas ligand interaction in liver transplantation.
The materials of this study were the 110 patients who underwent living-donor partial liver transplantation with end-stage liver diseases, including 16 patients with fulminant hepatic failure. The serum levels of soluble Fas ligand were serially measured with ELISA assay. TUNEL staining and immunohistochemical double staining of Fas, Fas ligand, CD-67, CD-20, CD-3 of the liver tissue were performed. The expression of mRNA of Fas and Fas ligand were analyzed with northern blot and RT-PCR.
The serum levels of soluble Fas ligand were elevated in the pretransplant patients with fulminant hepatic failure and biliary atresia, and postoperative patients of pediatric population. In the patients with fulminant hepatic failure, Fas ligand was expressed in the T-lymphocytes and Kupffer cells. The histochemical and the genetic analysis demonstrated that Fas expression was incremented in the biliary epithelium in patients with acute cellular rejection after transplantation, and the Fas ligand expressed infiltrating T lymphocytes as well as Kupffer cells.
These observations suggest that Fas/Fas ligand mediated apoptosis plays an important role in the development of fulminant hepatic failure, biliary atresia as well as posttransplant allograft rejection.
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