| Project/Area Number |
11470258
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
EGAWA Hiroto Kyoto University Transplant Surgery Associate Professor, 医学研究科, 助教授 (40293865)
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| Co-Investigator(Kenkyū-buntansha) |
UEMOTO Shinji Mie University Surgery Professor, 医学部, 教授 (40252449)
INOMATA Yukihiro Kumamoto University Pediatric Surgery Professor, 医学部, 教授 (50193628)
TANAKA Koichi Kyoto University Transplant Surgery Professor, 医学研究科, 教授 (20115877)
KIUCHI Tetsuya Nagoya University Transplant Surgery Professor, 医学研究科, 教授 (40303820)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | living donor liver transplantation / tolerance / immunosuppression withdrawal / humoral factors / cytokines / cellular factors / regulatory T cells / Tacrolimus / 免疫抑制剤 / 計画的離脱 / リンパ球混合試験 / 非特異的免疫寛容 / 免疫染色 / アポトーシス / Fas-Fasリガンド / 免疫抑制 / RT-PCR |
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| Research Abstract |
This study aims to clarify the mechanisms of clinical tolerance, to identify indicators for safe withdrawal of immunosuppression, and to increase the number of patients free from immunosuppression. Patients free from immunosuppression after living liver transplantation (IS free patients) were investigated, such as Th1 & Th2 cytokines, regulatory T cells. 1) IS free patients showed donor specific hypo-response in MLR and the Th1 cytokines were down regulated in IS free patients compared with third party MLR. 2) There was no specific feature in cytokine gene expression in liver biopsy tissue in IS free patients. 3) The serum of IS free patients showed nonspecific suppression in MLR but we could not isolate and identify the substance yet. 4) The number of CD4+/CD25+ cells and γ/δ TCR+ T cells were increased in IS free patients compared with healthy people. 5) In clinical, over 900 patients underwent living donor liver transplantation in our institution and the number of patients completely free from immunosuppression was increased from 15 in 1998 to 49 in 2002 and other 36 patients were under weaning protocol. We continue to study cellar factors as the most possible indicators for IS withdrawal.
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