| Project/Area Number |
11470280
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | NATIONAL CARDIOVASCULAR CENTER RESEARCH INSTITUTE |
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Principal Investigator |
NIIMI Hideyuki NATIONAL CARDIOVASCULAR CENTER, DEPT OF VASCULAR PHYSIOLOGY, DIRECTOR, 脈管生理部, 部長 (50026134)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Saburo DEPT OF VASCULAR PHYSIOLOGY, CHIEF, 脈管生理部, 室長 (00182436)
SEKI Junji DEPT OF BIOMEDICAL ENGINEERING, CHIEF, 生体工学部, 室長 (20163082)
KURO Masakazu DEPT.OF ANESTHESIOLOGY, DIRECTOR, 手術部, 部長
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | AUTOREGULATION / CARDIOPULMONARY BYPASS / INTRAVITAL MICROSCOPY / PIAL MICROCIRCULATION / RED CELL VELOCITY / VASODILATION / PULSATIVITY / CEREBRAL DISORDER / 蛍光ラベル / 血流可視化 / 微小血管径変化 / 白血球挙動 |
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| Research Abstract |
THIS STUDY INVESTIGATED CEREBRAL MICROCIRCULATORY CHANGES IN RAT WITH A CARDIOPULMONARY BAYPASS (CPB) AT NORMOTHERMIA TO ELUCIDATE CEREBROVASCULAR DISORDERS CAUSED BY SURGICAL OPERATION WITH CPB.IN THE RAT CPB SYSTEM, THE ARTERIAL PRESSURE WAS CHANGED FROM 50 TO 200 mmHg BY CHANGING THE PUMP FLOW-RATE.A NON-PULSATILE FLOW MODEL WAS DEVELOPED BY STOPPING CARDIAC BEAT USING A FIBRILATOR.THE PIAL MICROCIRCULATION WAS VISUALIZED USING FLUORESCENCE-LABELED RED CELLS AND DEXTRAN INJECTED INTRAVENOUSLY, AND WAS DIRECTLY OBSERVED UNDER A FLUORESCENCE VIDEOMICROSCOPY DURING CPB WITH/WITHOUT CARDIAC BEATING.BASED ON THE RECORDED VIDEOIMAGES, THE ARTERIOLAR DIAMETER AND RED CELL VELOCITY WERE EVALUATED WHERE SINGLE ARTERIOLES WITH APPROX.40μm DIAMETER WERE SELECTED AMONG THE PIAL ARTERIOLES.
THE PRESENT RESULTS SHOWED THAT 1) WHEN THE ARTERIAL PRESSURE CHANGED, ARTERIOLAR VASODILATION (OR VASOCONSTRICTION) APPEARED DURING PULSATILE FLOW BUT IT DISAPPEARED DURING NON-PULSATILE FLOW, AND 2) THE ARFTERIOLAR RED CELL VELOCITY INCREASED (DECREASED) LINEARLY DURING NON-PULSATILE FLOW AS WELL AS PULSATILE FLOW.THE FLOW-RATE WAS ALMOST CONSTANT AT A LARGE RANGE OF THE MEAN ARTERIAL PRESSURE FROM 60 TO 160 mmHg DURING PULSATILE FLOW, BUT THE FLOW-RATE INCREASED OR DECREASED DURING NON-PULSATILE FLOW WITH AN INCREASE OR DECREASE IN MEAN ARTERIAL PRESSURE, RESPECTIVELY.IT WAS SUGGESTED THAT PULSATIVITY MIGHT BE RESPONSIBLE FOR CEREBRAL AUTOREGULATION.
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