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Elucidation of the Mechanisms for Glutamate Release in Ischemic Neuronal Injury

Research Project

Project/Area Number 11470283
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionThe University of Tokyo

Principal Investigator

KIRINO Takaaki  The University Hospital, Neurosurgery, Professor, 医学部・附属病院, 教授 (90126045)

Co-Investigator(Kenkyū-buntansha) NOBUTAKA Kawahara  The University Hospital, Neurosurgery, Assistant Professor, 医学部・附属病院, 講師 (60214673)
川合 謙介  東京大学, 医学部・附属病院, 助手 (70260924)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2000: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1999: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordscerebral ischemia / glutamate transporter / knockout mice / gerbil / delayed neuronal death / GLT-1
Research Abstract

Elucidation of the Mechanisms for Glutamate Release in Ischemic Neuronal Injury
There have been an accumulating evidence that extracellular glutamate is contineously taken up and regulated in concentration by glutamate transporters. Especially, GLT-1 is specifically and widely distributed in forebrain area. Recently, GLT-1 has reported to release intracellular glutamate toward extracellular space in a condition such as ischemia.
To test this hypothesis and make sure the function of GLT-1 during ischemia, we determined extracellular glutamate concentration in gerbils with naive and ischemic condition by inhibition of glutamate transporter. In addition, we produced a focal cerebral ischemia by cauterization of the middle cerebral artery in mice with GLT-1 gene deleted and its wild type.
Results :
(1) We infused tPDC, an inhibitor of a glutamate transporter and collected extracellular fluid of gerbil brain by a microdialysis method. We confirmed that extracellular glutamate increased during an inhibition of a glutamate transporter. This result indicates that a glutamate transporter plays an important role in regulating extracellular glutamate concentration. As the next step, we induced a transient global ischemia to gerbils which were pre-infused with tPDC.We could not confirm the significant protective effect against neurons in CA1 sector of the hippocampus of the animals.
(2) We used GLT-1 gene knockout mice to examine whether ischemic neuronal death is accelerated and an infarct size is augmented. By producing a permanent focal cerebral ischemia, there was no differences in infarct volumes (homozygotes : 12.6±10.3, wild type : 12.6±6.8, Mean±SD mm^3). Since there is a possibility that the severity of an initial ischemic insult but not glutamate toxicity affected the neuronal cell death, we are ongoing to measure the infarct volumes by adopting ischemia-reperfusion models which are less severe than permanent ischemia.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Ide T,Takada K,Qui JH,Saito N,Kawahara N,Asai A and Kirino T: "Ubiquitin stress response in postischemic hippocampal neurons under nontolerant and tolerant conditions."J Cereb Blood Flow Metab. 19. 750-756 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawahara N,Mishima K,Higasa S,Taniguchi N,Tamura A and Kirino T: "The gene for heparin-binding epidermal growth factor-like growth factor is stress-inducible : its role in cerebral ischemia."J Cereb Blood Flow Metab. 19. 307-320 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 桐野高明: "脳血管障害の分子生物学"脳神経外科. 27. 111-118 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawai K,Kawahara N,Saito N,Nakatomi H,Ichi S and Kirino T: "Effect of selective blocker of Ca2+- permeable AMPA receptor in gerbil forebrain ischemia : In vivo verifyication of GluR2 hypothesis in delayed neuronal death of CA1 neurons."J Cereb Blood Flow Metab. 19. S458 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 川合謙介: "虚血性脳障害におけるグルタミン酸トランスポーターの役割に関する実験的研究"臨床成人病. 29. 1376-1377 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Asai A,Qui JH,Narita Y,Chi S,Saito N,Shinoura N,Hamada H,Kuchino Y and Kirino T: "High level calcineurin activity predisposes neuronal cells to apoptosis."J Biol Chem. 274. 34450-34458 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ide T, Takada K, Qui JH, Saito N, Kawahara N, Asai A and Kirino T: "Ubiquitin stress response in postischemic hippocampal neurons under nontolerant and tolerant conditions."J Cereb Blood Flow Metab. 19. 750-756 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawahara N, Mishima K, Higashiyama S, Taniguchi N, Tamura A and Kirino T: "The gene for heparin-binding epidermal growth factor-like growth factor is stress-inducible : its role in cerebral ischemia."J Cereb Blood Flow Metab. 19. 307-320 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kirino T: "Molecular biology in the cerebrovascular disease"No Shinkei Geka (in Japanese). 27. 111-118 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawai K, KAwahara N, Saito N, Nakatomi H, Ichi S and Kirino T: "Effect of selective blocker of Ca2+-permeable AMPA receptor in gerbil forebrain ischemia : In vivo verification of GluR2 hypothesis in delayed neuronal death of CA1 neurons."J Cereb Blood Flow Metab. 19(Suppl 1). S458 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawai K: "Experimental study of a role for the glutamate transporters in ischemic neuronal injury"Rinsho Seijin Byou (in Japanese). 29. 1376-1377 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Asai A, Qui JH, Narita Y, Chi S, Saito N, Shinoura N, Hamada H, Kuchino Y and Kirino T: "High level calcineurin activity predisposes neuronal cells to apoptosis."J Biol Chem. 274. 34450-34458 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ide T,Takada K,Qui JH,Saito N,Kawahara N,Asai A and Kirino T: "Ubiquitin stress response in postischemic hippocampal neurons under nontolerant and tolerant conditions."J Cereb Blood Flow Metab. 19. 750-756 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawahara N,Mishima K,Higasa S,Taniguchi N,Tamura A and Kirino T: "The gene for heparin-binding epidermal growth factor-like growth factor is stress-inducible : its role in cerebral ischemia."J Cereb Blood Flow Metab. 19. 307-320 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 桐野高明: "脳血管障害の分子生物学"脳神経外科. 27. 111-118 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawai K,Kawahara N,Saito N,Nakatomi H,Ichi S and Kirino T: "Effect of selective blocker of Ca2+-permeable AMPA receptor in gerbil forbrain ischemia : In vivo verifyication of GluR2 hypothesis in delayed neuronal death of CA1 neurons."J Cereb Blood Flow Metab. 19. S458 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 川合謙介: "虚血性脳障害におけるグルタミン酸トランスポーターの役割に関する実験的研究"臨床成人病. 29. 1376-1377 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Asai A,Qui JH,Narita Y,Chi S,Saito N,Shinoura N,Hamada H,Kuchino Y and Kirino T: "High level calcineurin activity predisposes neuronal cells to apoptosis."J Biol Chem. 274. 34450-34458 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Asai A,Qiu JH,Narita Y,Chi S,Saito N,Shinoura N,Hamada H,Kuchino Y,Kirino T: "High level calcineurin activity predisposes neuronal cells to apoptosis"J Biol Chem. 274. 34450-34458 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ide T,Takada K,Qiu JH,Saito N,Kawahara N,Asai A,Kirino T: "Ubiquitin stress response in postischemic hippocampal neurons under nontolerant and tolerant conditions"J Cereb Blood Flow Metab. 19. 750-756 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawahara N,Mishima K,Higashiyama S,Taniguchi N,Tamura A,Kirino: "The gene for heparin-binding epidermal growth factor-like growth factor stress-inducible : its role in cerebral ischemia"J Cereb Blood Flow Metab. 19. 307-320 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 桐野 高明: "脳血管障害の分子生物学"脳神経外科. 27. 111-118 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawai K,Kawahara N,Saito N,Nakatomi H,Ichi S,Kirino T: "Effect of selective blocker of Ca^<2+>-permeable AMPA receptor in gerbil forebrain ischemia : In vivo verification of GluR2 hypothesis in delayed neuronal death of CA1 neurons"J Cereb Blood Flow Metab. 19(Suppl 1). S458 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 川合 謙介: "虚血性脳障害におけるグルタミン酸トランスポーターの役割に関する実験的研究"臨床成人病. 29. 1376-1377 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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