| Project/Area Number |
11470293
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
KURATSU Jun-ichi Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (20145296)
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHIMA Hideo Kagoshima University, University Hospital, Faculty of Medicine, Assistant Professor, 医学部・附属病院, 講師 (70244134)
NIIRO Masaki Kagoshima University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (30172612)
横山 俊一 鹿児島大学, 医学部・附属病院, 講師 (90191519)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2001: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1999: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | Glioma / MCP-1 / neovascula rization / Macrophage / Invasion / statin / トロンビン |
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| Research Abstract |
Malignant gliomas are inevitably fatal, despite concerted, interdisciplinary efforts to improve the prognosis of patients with these tumors. The biological characteristics of malignant gliomas are their fast growth, neovascularization, and invasiveness into the surrounding brain. A great variety of molecules play important roles in these events. We studied the pathophysiology of malignant gliomas in Nan effort to develop novel treatment strategies to combat these malignant neoplasms.
We previously purified MCP-1 (monocyte chemoattractant protein-1) from glioma cells and confirmed that this protein participates significantly in the invasion of macrophages into glioma tissue. Therefore, we posited that control of malignant glioma growth may be possible via the regulation of molecules whose activities result in the characteristic features of malignant gliomas.
We subsequently established that MCP-1 and thrombin derived from glioma cells are involved in the neovascularization characteristic of these tumors. Using a conformation-epitope-recognizing mAb and a phase-display library, we succeeded in the cloning and functional characterization of the MCP-1 receptor gene and produced a peptide that mimics MCP-1 and expresses antagonistic activity. We also developed highly efficient electro-gene therapy for solid tumors arid suggest that our gratifying results will lead to the development of clinically applicable treatment strategies against malignant tumors.
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