• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of osteosarocma specific marker for detection of micro-metastasis

Research Project

Project/Area Number 11470304
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

HOTTA Tetsuo  NIIGATA UNIVERSITY, Medical Hospital Associate Professor, 医学部・附属病院, 助教授 (00272815)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥7,500,000 (Direct Cost: ¥7,500,000)
Keywordsosteosarocma / micro-metastasis / Cbfa1 / 0sf2 / peripheral blood / PCR-ELISA / ALP / Cbfal / 微少転移検出
Research Abstract

Clinically non-osteosarcoma tumors can be observed with osseous matrix, and it is difficult to distinguish such tumors from osteosarcomas only by histological examination. Because the treatment strategy for osteosarcoma is quite different from other malignant neoplasms, it is important to identify a specific marker for osteosarcoma. Some of osteosarcomas reveals elevation of serum alkaline phosphatase (ALP), so ALP was considered one of the tumor marker in osteosarcomas. However, we revealed some of clear cell sarcomas also showed high expression of serum ALP, and we considered ALP has relative low specificity in bone tumors.
Recently, Osf2/Cbfa1 (hereafter called Osf2), a member of the runt family of transcription factors, was identified as an activator forosteoblastic differentiation. In this study, we showed the expression of Osf2 mRNA only in osteosarcoma cell lines while not in non-osteosarcoma cell lines, suggesting that Osf2 mRNA expression is related to the osteoblastic phenotype of osteosarcoma cells. This result suggested that the expression of CBFA1 might be the specific tumor marker for osteosarcoma.
Circulating tumor cells in the blood play a central role in the metastatic process. There have been no reports describing the relationship between lung metastasis and circulating osteosarcoma cells. We developed a system with a polymerase chain reaction assay based on an enzyme-linked immunosorbent assay (PCR-ELISA) to detect circulating osteosarcoma cells in a mouse metastatic model. Osf2 was used as a target gene. One splicing variant of Osf2 mRNA was identified and its expression was restricted to the bones and osteosarcomas. The amount of the splicing variant of Osf2 mRNA was significantly higher in the blood of mice with metastasis than in the blood of the control group. The PCR-ELISA using Osf2 mRNA is a potential method to detect circulating osteosarcoma cells in peripheral blood.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Akira Ogose, et al.: "Elevation of serum alkaline phosphatases in clear cell chondrosarcoma of bone"Anticancer Research. Vol.21. 649-656 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi Hatano, et al.: "A PCR-ELISA assay for the detection of disseminated osteosarcoma cells in a mouse metastatic model"Journal of Orthopaedic Science. Vol.6. 269-275 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Ogose, et al: "Elevation of serum alkaline phosphatase in clear cell chondrosarcoma of bone"Anticancer Research. vol. 21. 649-656 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi Hatano, et al.: "A PCR-ELISA assay for the detection of disseminated osteosarcoma cells in a mouse metastasis model"Journal of Orthopaedic Science. vol. 6. 269-275 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 畠野宏史: "ヒト骨肉腫におけるCBFA1遺伝子の発現"日本整形外科学会雑誌. Vol72. S1014 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hatano H: "Expression of Cbfal gene in human malignant tumors"Transuctions of 44^<th> Annual Meeting Orthopedic Research Society.. 22. 824-825 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hatano H: "A PCR-ELISA Assay for the Detection of Disseminated Osteosarcoma Cells in a Mice Metastatic Model"Journal of Orthopaedic Science. (In press). (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi