| Project/Area Number |
11470311
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KATOH Shinsuke (2000) The University of Tokushima, School of Medicine, Associate Professor, 医学部, 助教授 (30243687)
井形 高明 (1999) 徳島大学, 医学部, 教授 (80108860)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYA Yutaka The University of Tokushima, School of Medicine, Professor, 医学部, 教授 (50136222)
ITAKURA Mitsuo The University of Tokushima, Institute for Genome Research, Professor, ゲノム機能解析センター, 教授 (60134227)
TAMAKI Toshiaki The University of Tokushima, School of Medicine, Professor, 医学部, 教授 (80179879)
IKATA Takaaki The University of Tokushima, School of Medicine, Professor Emeritus, 名誉教授 (80108860)
FUKUZAWA Kenji The University of Tokushima, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (90035551)
加藤 真介 徳島大学, 医学部, 助教授 (30243687)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | spinal cord / oxidative stress / steroid / superoxide dismutase / neurotrophic factor / nerve graft / SOD |
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| Research Abstract |
Recent researches showed the involvement of damages of the vascular endothelial cells in the secondary pathological changes after mechanical trauma. In the present studies, we compared the effects of lecithinized superoxide dismutase(PC-SOD)and MPSS which have been known to reduce the injury as anti-oxidants.
At first, we investigated the distribution of the glucocorticoid receptors in the noramal rat spinal cord using light microscopic immunohistochenistry. We found that the receptors distributed in all laminae of Rexed. In the dorsal horn, the receptor was found in the intrinsic neuron which contained enkephalin.
The effects of MPSS and PC-SOD was compared on recovery of hindlimb motor functiorl and expression of mRNA of pro-inflammatory and neurotrophic genes in the rat spinal cord injury. Hindlimb motor function of the PC-SOD group better than that of MPSS group. PC-SOD and MP had down-regulatory effects on mRNA expression of interleukin-1β(IL-1β), intercellular adhesion molecule-1, and inducible-nitricoxide synthetase after spinal cord compression at 3, 6, and 24 h, respectively, and on the lipid peroxide level 1 h after injury. The level of neurotrophin-3(NT-3)mRNA increased from 6 h after SCI and reached a maximum after 48 h. NT-3 mRNA level was enhanced by PC-SOD treatment, but not by MPSS treatment. Microinjection of PC-SOD into the normal rat spinal cord did not increase mRNA of NT-3, which indicated that PC-SOD did not increase NT-3 mRNA directly.
Thus, the effect of MPSS in suppressing thesepro-inflammatory genes expression was more than that of PC-SOD.The difference in motor function in the early and later stage maybe partially due to differences in expression of IL-1β and NT-3 after either treatment, through an IL-1β-dependent or NT-3-mediated repair response. We should take the effects on the reparative processes into account when the pharmacological treatment protocol is designed.
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