| Project/Area Number |
11470339
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yokohama City University Grant-in Aid for Scientific Research (B)(2) |
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Principal Investigator |
KUBOTA Yoshinobu Yokohama City Univ., School of Medicine, Professor, 医学部, 教授 (10106312)
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| Co-Investigator(Kenkyū-buntansha) |
YAO Masahiro Yokohama City Univ., School of Medicine, Associate Professor, 医学部, 助教授 (00260787)
UEMURA Hiroji Yokohama City Univ., School of Medicine, Associate Professor, 医学部, 講師 (50244439)
穂坂 正彦 横浜市立大学, 医学部, 教授 (30106330)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Prostate cancer / Hormone Refractory / Differential Display / OSM-? gene / nmt55 gene / Liprin? gene / 再熱 / 再燃現象 / 遺伝子発現 |
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| Research Abstract |
In this research, new human genes associated with hormone refractory prostate cancer were tried to be identified by applying differential display PCR methodology. The RNA of human Prostate cancer and normal prostate tissue was analyzed for differentially expressed gene. We have clones more than one hundred possible genes. And OSM-? and nmt55 genes were identified as important genes.
OSM-β gene is one of the tyrosine kinase type receptor family genes. This gene was shown to be highly expressed in hormone refractory prostate cancer and suggested to be involves in androgen independent growth characteristics.
The nmt55 gene is coded as one of the transcriptional factors. We have shown that the expression of this gene was closely associated with the expression of androgen receptor. This gene was shown to be disappear last in hormone refractory prostate cancer and suggested to be associated with androgen independent growth regulation.
We have also analyzed the RNA of LNCaP human prostate cancer cells treated with dihydrotestosterone for differentially expressed gene. Two important genes Liprin?-2 and NKx 3.1 like gene were cloned.
Liprinα-2 gene expression was shown to be down-regulated by androgen and this gene might be associated with the induction of apoptosis after androgen deprivation. Since Liprinα-2 gene expression disappeared completely lost in androgen independent prostate cancer cell line (PC-3, DU-145) or hormone refractory prostate cancer tissues, this gene is suggested to play a roll in the induction of hormone independent prostate cells.
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