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BASIC RESEARCHES ON GENE TRANSFER FOR THE PROPHYLAXIS OF ACOUSTIC TRAUMA

Research Project

Project/Area Number 11470359
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKeio University

Principal Investigator

KANZAKI Jin  KEIO UNIV. SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (00051441)

Co-Investigator(Kenkyū-buntansha) SHIZUKI Ken  KEIO UNIV. SCHOOL OF MEDICINE LECTURER, 医学部, 助手 (10276355)
OGAWA Kaoru  KEIO UNIV. SCHOOL OF MEDICINE ASSIST PROFESSOR, 医学部, 講師 (00169179)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2000: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsACOUSTIC TRAUMA / GENE TRANSFER / GDNF / ADENO VIRUS / SHAKER-2 MOUSE / GUINEA PIG / COCHLEA / CDNF / ヘルペスウイルス / マウス
Research Abstract

There are considerable evidences suggesting that cochlear hair cells are first damaged by acoustic trauma followed by secondary degeneration of the cochlear nerve. GDNF, a glial derived neurotrophic factor, has been known to exert a protective or prophylactic role against acoustic trauma. On the other hand, gene therapy is a powerful technology that may be applied for treating inner ear disease including acoustic trauma. Several studies have shown successful transduction of inner ear cells using viral vectors. In our first experiment, an infection of adenovirus vector was studied in the Shaker-2 mouse cochleae and vestibules. Adenovirus vector was found in the hair cells and supporting cells. There was no significant difference in the expression among the homo-, hetero- and wild types. The expression in the hair cells decreased from P1 to P5. In the second experiment, we investigated the distribution pattern of transferred GDNF in the cochlea using guinea pigs. As a result, GDNF was expressed in the spiral ganglion cells. GDNF was not expressed in the contra-lateral cochlea. In 5 weeks after the transfer of GDNF, more than 150〜200% of the spiral ganglion cells were survived compare to the control, which showed a statistically significant difference. Based upon these results we can conclude that the expression of GDNF induced by gene transfer has a role on protection or prophylaxis against the degeneration of spiral ganglion cells.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Hoya N, Ogawa K, Inoue Y, Kanzaki J: "Macrodialytic measurement of Ach in perilymph of guinea pig"Neurosci Lett. 311. 206-208 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohba T, Ogawa K, Inoue Y, Kanzaki J: "Heterogeneity of PLC in the cochlea of the guinea pig"ORLJ Otorhinolaryngol Relat Spec. 63. 272-279 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito H, Ogawa K, Inoue Y, Kanzaki J, Hara: "Photo-induced ischemia of guinea pig"ORLJ Otorhinolaryngol Relat Spec. 63. 148-154 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M, Ogawa K, Inoue Y, Satou M, Kanzaki J: "Effects of NPY on cochlear blood flow"Acta Otolaryngol. 121. 573-578 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shizuki K, Ogawa K, Matsunobu T, Kanzaki: "Expression of c-Fos after noise-induced temporary threshold shift in the *"Neurosci Lett. 320. 73-76 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanzaki S, Ogawa K, Camper SA, Raph: "First generation and advanced adenoviral vector mediated mouse inner *"Hear Res. (in printing).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hoya N, Ogawa K, Inoue Y, Kanzaki J: "Microdialytic measurement of Ach in perilymph of guinea pig"Neurosci Lett. 311. 206-8 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohba T, Ogawa K, Inoue Y, Kanzaki J: "Heterogeneity of PLC in the cochlea of the guinea pig"ORL J Otorhinolaryngol Relat Spec. 63. 272-279 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito H, Ogawa K, Inoue Y, Kanzaki J, Harada T, Hoya N: "Photo-induced ischemia of guinea pig"ORL J Otorhinolaryngol Relat Spec. 63. 148-154 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito M, Ogawa K, Inoue Y, Satou M, Kanzaki J: "Effects of NPY on cochlear blood flow"Acta Otolaryngol. 121. 573-578 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shizuki K, Ogawa K, Matsunobu T, Kanzaki J, Ogita K: "Expression of c-Fos after noise-induced temporary threshold shift in the guinea pig cochlea"Neurosci Lett. 320. 73-76 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanzaki S, Ogawa K, Camper SA, and Raphael Y: "First generation and advanced adenoviral vector mediated mouse inner ear"Hear Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary

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Published: 1999-04-01   Modified: 2016-04-21  

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