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Development of Local Drug Therapy on the Inner Ear

Research Project

Project/Area Number 11470360
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKansai Medical University

Principal Investigator

YAMASHITA Toshio  Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (10077654)

Co-Investigator(Kenkyū-buntansha) IWAI Hiroshi  Kansai Medical University, Faculty of Medicine, Associate Professor, 医学部, 講師 (10232638)
DOI Tadashi  Kansai Medical University, Faculty of Medicine, Associate Professor, 医学部, 講師 (60288826)
KURIYAMA Hiromichi  Kansai Medical University, Faculty of Medicine, Associate Professor, 医学部, 講師 (90268350)
KOMEDA Mototane  Kansai Medical University, Faculty of Medicine, Assistant, 医学部, 助手 (20319619)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥3,600,000 (Direct Cost: ¥3,600,000)
Keywordsosmotic pump / drug delivery / cochlea / kanamycin / ototoxicity / steroid / NO / カナマイシン / カルシウムイオン / NO合成酵素 / 薬剤性難聴 / 局所治療 / モルモット / 蝸牛 / ABR
Research Abstract

We set the researching project regarding to the local drug therapy on the inner ear. On the entire project, an animal model with drug-infusing system to the unilateral cochlea was used. Drug infusion was perfonned with an osmotic pump connecting to catheter which lead drug to the cochiear scala tynlpani. Infusion of artificial perilymph as a control study demonstrated no damage of cochlea. in both electrophysiology and cochlear cytology. Ototoxicity modei was made with kanamycin and ethacrynic acid on the bilaterai cochlea.
As an experimental agent, ciexamethasone showed protective effect, against kanamycin-induced ototoxicity in the cochlca. The group of drug infusion two days prior to kanamycin admininistration led more protective effect than the group of drug infusion started the same day as kanamycin adrninistration. The other agent, iNOS inhibitor sbowed the protective effect on the kanamycin- induced trauma. Additional experimcnt using NO specific fluoresccnt dye. DAP-2DA showed reduction of kanamycin-induced NO synthesis on experirncntal side comparing to that on control side. We have also evaluated the effects on calcium free periiymphatic solution. NMDA receptor antagomst and cocktail of neurotrophirls, and determined their adequate concentrations.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Himeno C et al.: "Intra-cochlear administration of dexamethasone attenuates aminoglycoside ototoxicity in the guinea pig"Hearing Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 山下 敏夫: "蝸牛の音受容障害 -光学的解析による研究-"メディカ出版. 211 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Himeno C, Komeda M, Izumikawa M, Takemura K, Yagi M, Weiping Y, Doi T, Kuriyama H, Miller JM, Yamashita T: "Intra-cochier administration of dexamethasone attcnuates aminoglycoside ototoxicity in the guinea pig"Hearing Resarch. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary

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Published: 1999-04-01   Modified: 2016-04-21  

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