| Project/Area Number |
11470383
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
UMEMOTO Toshio Kanagawa Dental College, Department of Dentistry, Professor, 歯学部, 教授 (20067036)
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA Nobushiro Kanagawa Dental College, Lecturer, 歯学部, 講師 (20247315)
WATANABE Kiyoko Kanagawa Dental College, Lecturer, 歯学部, 講師 (70148021)
YOSHIMOTO Hisashi Kanagawa Dental College, Assistant Professor, 歯学部, 助教授 (60084787)
TAKAHASHI Yusuke Kanagawa Dental College, Assistant, 歯学部, 助手 (20267511)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | Porphyromonas gingivalis / Periodontopathic bacteria / major fimbriae / minor fimbriae / inactivated fimA gene / fimA transformed strain / cell attachment / bone resorption activity / メジャーフィンブリエ / マイナーフィンブリエ / 細胞付着能 / mfal不活化 / fimA不活化株 / mfa1不活化株 / 線毛 |
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| Research Abstract |
We previously reported that the presence of two different kinds of fimbriae expressed on the cell surface of Porphyromonas gingivalis ATCC 33277. The initial event in most infectious diseases involves adhesion of pathogens to host tissues and subsequent invasion by the pathogens. To define the role of fimbriae in Porphyromonas gingivalis adherence to and invasion of epithelial cells, we have constructed fimbrial mutants. The involvement of P. gingivalis fimbriae in the invasion process and alveolar bone resorption in rats were examined.
Inactivated mutants of 41-K fimbrillin gene (fimA) and/or the 67-K fimbrillin gene (mfal) by a homologous recombination technique and compared among fimA mutant (MPG1), mfal mutant (MPG67), and double knockout mutant (MPG4167). Adherence and invasion of P. gingivalis was assessed in human oral epithelial KB cells. We used a rat model to examine the role of each type of fimbriae in alveolar bone loss by oral infection.
The adherence and invasion levels of the mutants were lower than the wild-type strain. The bone loss of rats infected with the MPG1 was higher than that of those infected with MPG67. Moreover, the bone loss of rats infected with the double knockout mutant was significantly decreased compared to that of rats infected with the wild-type strain.
Data from this study suggest that not only the 41-K fimbrial protein, but also the 67-K fimbrial protein play important roles in the pathogenesis of periodontal disease.
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