Development of preventive method for dental caries by using phosphoryl-oligosaccharides
| Project/Area Number |
11470384
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | National Institute of Infectious Diseases |
|---|
Principal Investigator |
IMAI Susumu National Institute of Infectious Diseases, Department of Oral Science, Senior researcher, 口腔科学部, 主任研究官 (80072958)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NISIZAWA Tosiki National Institute of Infectious Diseases, Department of Oral Science, Chief of Laboratory, 口腔科学部, 室長 (00072942)
|
|---|
| Project Period (FY) |
1999 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥3,700,000 (Direct Cost: ¥3,700,000)
|
|---|
| Keywords | phosphoryl-oligosaccharides / mutans streptococci / dental caaries / acid fermentation / artificial mouth system / demineralization / エナメル質脱灰 |
|---|
| Research Abstract |
The purpose of this study was to develop the preventive methods for dental caries by using functional new oligosaccharides which are not only less fermentable by mutans streptococci, but also inhibitory against dental caries through the suppression of organic acids production and promotion of re-mineralization. Phosphoryl-oligosaccharides (POs) were produced from potato starch by using alfa-amylase. Effect of POs on the precipitation of calcium-phosphate was examined. POs inhibited the precipitation of calcium-phosphate and promoted the solubility of calcium. POs itself were not fermented by mutans streptococci such as S.mutans and S.sobrinus. Moreover, POs inhibited pH decrease of medium due to the fermentation of sucrose by mutans streptococci in dose-dependent manner. It was suggested that inhibition of pH decrease by POs was ascribed to buffering action of POs. In artificial mouth system, POs inhibited the accumulation of artificial biofilm on the enamel slab surface, pH decrease underneath the artificial biofilm and de-mineralization of enamel by mutans streptococci. It was confirmed from these results that POs possessed appropriate characters for the caries preventive agents, and was applicable to various measures for the prevention of dental caries.
|
Report
(4 results)
Research Products
(15 results)
-
-
[Journal Article] Feasibility of eradication of mutans streptococci from oral cavities.2004
Author(s)
Nomura Y, Takeuchi H, Kaneko N, Matin, K, Iguchi R, Toyoshima Y, Kono Y, Ikemi T, Imai S, Nisizawa T, Fukushima K, Hanada N.
-
Journal Title
Journal of oral science 46
Pages: 179-183
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
-
-
-
-
-
[Journal Article] Clinical study of mutans streptococci using 3DS and monoclonal antibodies.2001
Author(s)
Takeuchi H, Fukushima K, Senpuku H, Nomura Y, Kaneko N, Yano A, Morita E, Imai S, Nisizawa T, Kono Y, Ikemi T, Toyoshima Y, Hanada N.
-
Journal Title
Jpn J.Infect.Dis. 54
Pages: 34-36
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] New dental drug delivery system for removing mutans streptococci from the oral cavity : effect on oral microbial flora.2000
Author(s)
Takeuchi H, Senpuku H, Matin K, Kaneko N, Yusa N, Yoshikawa E, Ida H, Imai S, Nisizawa T, Abei Y, Kono Y, Ikemi T, Toyoshima Y, Fukushima K, Hanada N.
-
Journal Title
Jpn J.Infect.Dis. 53
Pages: 211-212
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
