| Project/Area Number |
11470385
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KUBOKI Yoshinori Hokkaido Univ., Grad.Scho.of Dent., Pro., 大学院・歯学研究科, 教授 (00014001)
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| Co-Investigator(Kenkyū-buntansha) |
FUJISAWA Ryuichi Hokkaido Univ., Grad.Sch.of Dent., instr., 大学院・歯学研究科, 助手 (40190029)
MIZUNO Morimichi Hokkaido Univ., Grad.Sch.of Dent., Instr., 大学院・歯学研究科, 助手 (10125354)
OHATA Noboru Hokkaido Univ., Grad.Sch.of Dent., Prof., 大学院・歯学研究科, 教授 (60002185)
ITOH Hideaki The Japan Steel Works, Ltd., Muroran, Principal Researcher, 室蘭研究所, 主任研究員
TAKITA Hiroko Hokkaido Univ., Grad.Sch.of Dent., Instr., 大学院・歯学研究科, 助手 (30125330)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2000: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1999: ¥8,300,000 (Direct Cost: ¥8,300,000)
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| Keywords | periodontal regeneration / cementum regeneration / BMP / FGF / tissue engineering / titanium mesh / hydroxyapatite coating / laser perforated membrane |
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| Research Abstract |
The aims of this study were to extend the regeneration of periodontal tissues in the denuded roots of replanted teeth and in the artificial roots of teeth. Recombinant human BMP-2 was combined with fibrous collagen membrane(FCM)as reported(FCM/BMP). As a replanting system, premolars of beagle dogs were carefully extracted and treated. Before replantation, their periodontal ligaments(PL)and cementum were partially removed and were covered with FCM/BMP.Although the characteristic perpendicular collagen fibers were histologially observed between the newly formed cementum and alveolar bone, extent of regeneration was limited, often accompanied by ankyosis and resorption of roots. Then we devised a artificial root system, in which a titanium rod was surrounded by titanium-fiber mesh(TM-TR). This device(TM-TR)effectively induced layered bone(cementum-like tissue)within the Ti-mesh, when combined with rhBMP-2 and implanted into rat skin. Two other BMP-carriers, honeycomb hydroxyapatite and CPSA bioglass fibers were developed. In order to prevent ankylosis, we also devised laser-perforated collagen membrane. Together with new artificial teeth and new BMP carrier devices, dental implant with periodontal ligaments became ready-to-test stage of development.
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