• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

STUDY ON METABOLIC SYSTEMS FOR PEPTIDES AND AMINO ACIDS OF PERIODONTAL PATHOGENS, PORPHYROMONAS AND PREVOTELLA.

Research Project

Project/Area Number 11470386
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionTohoku University

Principal Investigator

TAKAHASHI Nobuhiro  Graduate School of Dentistry, Tohoku University, Professor, 大学院・歯学研究科, 教授 (60183852)

Co-Investigator(Kenkyū-buntansha) SATO Takuichi  Graduate School of Dentistry, Tohoku University, Assist. Prof., 大学院・歯学研究科, 助手 (10303132)
IWAMI Yoshimichi  Graduate School of Dentistry, Tohoku University, Lecturer, 大学院・歯学研究科, 講師 (60005030)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥6,600,000 (Direct Cost: ¥6,600,000)
KeywordsP. gingivalis / P. intermedia / PEPTIDE METABOLISM / ASPARTATE / GLUTAMATE / DIPEPTIDE / METABOLIC ENZYMES / PCR-RFLP / Porphyromonas gingivalis / Prevotella intermedia / Fusobacterium nucleatum / PCR-RELP法 / Dipeptidyl peptidase / Dipeptidase / アミノ酸代謝 / ブチル酸 / プロピオン酸
Research Abstract

1. Peptides and amino acids as sources for the growth and the energy production of Porphyromonas gingivalis (Pg) and Prevotella intermedia(Pi)
Periodontal pathogens, P. gingivalis and P. intermedia utilized peptides, especially peptides containing aspartate and/or glutamate, as the sources of their growth and energy production. In addition, P. gingivalis preferentially utilized aspartylaspartate and glutamylglutamate, while P. intermedia utilized aspartate and aspartylaspartate. The observation that both bacteria had no dipeptidase activity on their cell surfaces indicates that they incorporate dipeptides through a dipeptide-specific transport system. These findings suggest the existence of segregation or competition between P. gingivalis and P. intermedia for their nutrients.
2. Metabolic pathways for peptides and amino acids of P. gingivalis and P. intermedia
Metabolic system for peptides and amino acids of P. gingivalis and P. intermedia consists of the following steps: (l) peptide uptake (2) hydrolysis of peptide into amino acid (3) deamination of amino acid into 2-oxoacid (4) oxidation of 2-oxoacid into acyl-CoA and (5) production of short-chain fatty acid. 2-oxoacid oxidoreductases, which catalyze the reaction (4), utilized an artificial electron acceptor, methylviologen. This property is similar to that of enzymes found in archea.
3. Molecular biological method for identification of periodontal pathogens
From the ecological point of view as described 1, a molecular biological method for bacterial identification, was developed to know the distribution in dental plaque of periodontal pathogens, P. gingivalis and P. intermedia. A new method, PCR-RFLP based on bacterial 16S ribosomal RNA gene, was found to be not only simple and fast but also more reliable than the conventional method such as biological and morphological tests.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] Nobuhiro Takahashi: "Acid-induced acidogenicity and acid tolerance of non-mutans streptococci"Oral Microbiol Immunol. 14. 43-48 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobuhiro Takahashi: "Effect of pH on the glucose and lactate metabolisms of Actinomyces naeslundii"Oral Microbiol Immunol. 14. 60-65 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobuhiro Takahashi: "Glucose and lactate metabolism by Actinomyces naesllundii"Critic Rev Oral Biol Med. 10. 504-518 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masako Higuchi: "Functions for two types of NADH oxidases in energy metabolism and oxidative stress of Streptococcus mutans"J Bacteriol. 181. 5940-5947 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobuhiro Takahashi: "Pathways for amino acid metabolism by Prevotella intermedia and Prevotella nigrescens"Oral Microbiol Immunol. 15. 96-102 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobuhiro Takahashi: "Glucose metabolism by Prevotella intermedia and Prevotella nigrescens"Oral Microbiol Immunol. 15. 188-195 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasuhito Yamamoto: "Characterization of the Streptococcus mutans pyruvate formate-lyase (PFL)-activating enzyme gene by complementary reconstitution of the in vitro PFL-reactivating system"Infect Immun. 68. 4773-4777 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobuhiro Takahashi: "Metabolic pathways for cytotoxic end-product formation from glutamate-and aspartate-containing peptides by Porphyromonas gingivalis"J Bacteriol. 182. 4704-4710 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshimichi Iwami: "Rate-limiting steps of glucose and sorbitol metabolism in Streptococcus mutans cells exposed to air"Oral Microbiol Immunol. 15. 325-328 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] JingPing Hu: "Copidis Rhizoma in hibits growth and proteolytic activity of oral bacteria"Oral Dis. 6. 297-302 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Junko Matsuyama: "Comparison between 16S rRNA genes PCR-RFLP analysis and biochemical tests for identification of Actinomyces naeslundii"Int J Oral Biol. 25. 87-91 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takuichi Sato: "16S rRNA genes PCR-RFLP analysis for rapid identification of oral anaerobic gram-positive bacilli"Int J Oral Biol. 25. 83-86 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshimichi Iwami: "The time-course of acid excretion, levels of fluorescence dependent on cellular NADH and glycolytic intermediates of Streptococcus mutans cells exposed and not exposed to air at the coexistence of glucose and sorbitol"Oral Microbiol Immunol. 16. 34-39 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shoko Takahashi-Abbe: "Inhibitory effect of sorbitol on sugar metabolism of Streptococcus mutans in vitro and on acid production in dental plaque in vivo"Oral Microbiol Immunol. 16. 94-99 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobuhiro Takahashi: "Preferential utilization of dipeptides by Porphyromonas gingivalis"J Dent Res. 80. 1425-1429 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaoru Saito: "Effects of glucose on formation of cytotoxic end-products and proteolytic activity of Prevotella intermedia, Prevotella nigrescens and Porphyromonas gingivalis"J Periodontal Res. 36. 355-360 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobuhiro Takahashi: "Dipeptide utilization by periodontal pathogens, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens and Fusobacterium nucelatum"Oral Microbiol Immunol. 17. 50-54 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi N., Yamada T.: "Acid-induced acidogenicity and acid tolerance of non-mutans streptococci"Oral Microbiol. Immunol.. 14. 43-48 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi N., Yamada T.: "Effect of pH on the glucose and lactate metabolisms of Actinomyces naeslundii"Oral Microbiol. Immunol.. 14. 60-65 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi N., Yamada T.: "Glucose and lactate metabolism by Actinomyces naesllundii"Critic. Rev. Oral Biol. Med.. 10. 504-518 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Higuchi M., Yamamoto Y., Poole L.B., Shimada M., Sato Y., Takahashi N., Kamio Y.: "Functions for two types of NADH oxidases in energy metabolism and oxidative stress of Streptococcus mutans"J. Bacteriol.. 181. 5940-5947 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi N., Yamada T.: "Pathways for amino acid metabolism by Prevotella intermedia and Prevotella nigrescens."Oral Microbiol. Immunol.. 15. 96-102 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi N., Yamada T.: "Glucose metabolism by Prevotella intermedia and Prevotella nigrescens."Oral Microbiol. Immunol.. 15. 188-195 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto Y., Sato Y., Takahashi-Abbe S., Takahashi N., Kizaki H.: "Characterization of the Streptococcus mutans pyruvate formate-lyase (PFL)-activating enzyme gene by complementary reconstitution of the in vitro PFL-reactivating system"Infect. Immun.. 68. 4773-4777 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi N., Sato T., Yamada T.: "Metabolic pathways for cytotoxic end-product formation from glutamate- and aspartate-containing peptides by Porphyromonas gingivalis"J. Bacteriol.. 182. 4704-4710 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iwami Y., Takahashi-Abbe S., Takahashi N., Abbe K., Yamada T.: "Rate-limiting steps of glucose and sorbitol metabolism in Streptococcus mutans cells exposed to air."Oral Microbiol. Immunol.. 15. 325-328 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hu J.P., Takahashi N., Yamada T.: "Copidis Rhizoma inhibits growth and proteolytic activity of oral bacteria"Oral Diseases. 6. 297-302 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuyama J., Sato T., Takahashi N.: "Comparison between 16S rRNA genes PCR-RFLP analysis and biochemical tests for identification of Actinomyces naeslundii"Int. J. Oral Biol.. 25. 87-91 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sato T., Matsuyama J., Takahashi N.: "16S rRNA genes PCR-RFLP analysis for rapid identification of oral anaerobic gram-positive bacilli"Int. J. Oral Biol.. 25. 83-86 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iwami Y., Takahashi-Abbe S., Abbe K., Takahashi N., Yamada T., Kano N., Mayanagi H.: "The time-course of acid excretion, levels of fluorescence dependent on cellular NADH and glycolytic intermediates of Streptococcus mutans cells exposed and not exposed to air at the coexistence of glucose and sorbitol"Oral Microbiol. Immunol.. 16. 34-39 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi-Abbe S., Abbe K., Takahashi N., Tamazawa Y., Yamada T.: "Inhibitory effect of sorbitol on sugar metabolism of Streptococcus mutans in vitro and on acid production in dental plaque in vivo"Oral Microbiol. Immunol.. 16. 94-99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi N., Sato T.: "Preferential utilization of dipeptides by Porphyromonas gingivalis"J. Dent. Res.. 80. 1425-1429 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito K., Takahashi N., Horiuchi H., Yamada T.: "Effects of glucose on formation of cytotoxic end-products and proteolytic activity of Prevotella intermedia and Prevotella nigrescens"J. Periodontal Res.. 36. 355-360 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi N., Sato T.: "Dipeptide utilization by periodontopathic bacteria, Porphyromonas, Prevotella and Fusobacterium"Oral Microbiol. Immunol.. 17. 50-54 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobuhiro Takahashi: "Dipeptide utilization by periodontal pathogens, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens and Fusobacterium nucelatum"Oral Microbiol Immuno1. 17(1). 50-54 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nobuhiro Takahashi: "Preferential utilization of dipeptides by Porphyromonas gingivalis"J Dent Res. 80(5). 1425-1429 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kaoru Saito: "Effects of glucose on formation of cytotoxic end-products and proteolytic activity of Prevotella intermedia, Prevotella nigrescens and Porphyromonas gingivalis"J Periodontal Res. 36(6). 355-360 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takuichi Sato: "Rapid identification of cariogenic bacteria by 16SrRNA genes PCR-RFLP analysis"Cariology Today. 2(1). 8-13 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤拓一: "16S rRNA genes PCR-RFLP法を用いた口腔細菌の迅速同定"歯基礎誌. 43(5). 532 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nobuhiro Takahashi: "Utilization of dipeptides by periodontal pathogens, Porphyromonas, Prevotella and Fusobacterium"J Dent Res. 80. 677 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] N Takahashi: "Glucose metabolism by Prevotella intermedia and Prevotella nigrescens"Oral Microbiology and Immunology. 15(3). 188-195 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N Takahashi: "Pathways for amino acid metabolism by Prevotella intermedia and Prevotella nigrescens"Oral Microbiology and Immunology. 15(2). 96-102 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N Takahashi: "Metabolic pathways for cytotoxic end-product formation from glutamate- and aspartate-containing peptides by Porphyromonas gingivalis"Journal of Bacteriology. 182(17). 4704-4710 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y Yamamoto: "Characterization of the Streptococcus mutans pyruvate formate-lyase (PFL)-activating enzyme gene by complementary reconstitution of the in vitro PFL-reactivating system."Infection and Immunity. 68(8). 4773-4777 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y Iwami: "Rate-limiting steps of glucose and sorbitol metabolism in Streptococcus mutans cells exposed to air."Oral Microbiology and Immunology. 15(5). 325-328 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] JP Hu: "Copidis Rhizoma inhibits growth and proteolytic activity of oral bacteria"Oral Diseases. 6. 297-302 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 高橋信博: "歯肉縁下プラークという生態系.先端医療シリーズ・歯科医学2 歯周病"岡田宏,石川烈,村山洋二(監修).先端医療技術研究所. 9 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N. Takahashi: "Glucose metabolism by Prevotella intermedia and Prevotella nigrescens"Oral Microbiology and Immunology. 15. in press (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] N. Takahashi: "Pathways for amino acid metabolism by Prevotella intermedia and Prevotella nigrescens"Oral Microbiology and Immunology. 15(2). 96-102 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] N. Takahashi: "Glucose and lactate metabolism by Actinomyces naeslundii"Critical Reviews in Oral Biology & Medicine. 10(4). 487-503 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] N. Takahashi: "Effects of pH on the glucose and lactate metabolisms by the washed cells of Actinomyces naeslundii under anaerobic and aerobic conditions"Oral Microbiology and Immunology. 14(2). 60-65 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] N. Takahashi: "Pathways for amino acid and glucose metabolism by oral prevotella"Journal of Dental Research. 78. 259 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 高橋信博: "Porphyromonas gingivalis のペプチド代謝:細胞毒性代謝産物の産生経路とそれに関わる代謝酵素"歯科基礎医学会雑誌. 41(5). 403 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi