| Project/Area Number |
11470395
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
SAKAMOTO Wataru Hokkaido University, Graduate School of Dental Medicine, Asso.Prof., 大学院・歯学研究科, 助教授 (30001952)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIHIRA Jun Hokkaido University, Graduate School of Medicine, Asso.Prof., 大学院・歯学研究科, 助教授 (30189302)
KAWANAMI Masamitsu Hokkaido University, Graduate School of Dental Medicine, Asso.Prof., 大学院・歯学研究科, 助教授 (10133761)
KOHGO Takao Hokkaido University, Graduate School of Dental Medicine, Prof., 大学院・歯学研究科, 教授 (80001949)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Macrophage / Macrophage migration inhibitory factor (MIF) / Fibroblast / Periodontal disease / Lipopolysaccharide / TNF-α / IL-6 / PGE_2 |
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| Research Abstract |
Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine released by macrophages, T-cells, and the pituitary gland during inflammatory responses. In order to clarify the pathophysiological roles of MIF in periodontal disease, we investigated relationship between MIF and a variety of inflammatory mediators, such as PGE_2, IL-6, TNF-α, and matrix metalloproteinase in gingival tissues. MIF was expressed in both normal and inflamed gingival tissues. Interestingly, LPS-injection led to increased serum levels of IL-6 (12.6 ± 4.2 ng/ml) and TNF-α (4.6 ± 3.8 ng/ml), although these cytokines were not detceted in normal rat serum (less than 30 pg/ml). However, MIF level was hardly increased by LPS injection (417.7±209.9 ng/ml in non-injection ; 427.1 ± 53.0 ng/ml in LPS-injection). On the other hand, it was clarified that MIF secretion seems to be due to mainly cell-death and subsequently leakage of intracellular MIF, by analyses of trypan blue exclusion, formazan formation, morphological changes, and Westren blot in human cultured fibroblasts stimulated with H_2O_2 and lipopolysaccharides. These results indicate that MIF secretion differs from other inflammatory cytokines such as IL-6 and TNF-α.
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