| Project/Area Number |
11470399
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
病態科学系歯学(含放射線系歯学)
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
KITANO Motoo Kagoshima University Dental School, Professor, 歯学部, 教授 (10142118)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRAYAMA Yoshikazu Kagoshima University Dental School, Research Associate, 歯学部, 助手
SEMBA Ichiro Kagoshima University Dental School, Associate Professor, 歯学部, 助教授 (60145505)
五味 暁憲 鹿児島大学, 歯学部, 助手 (10325798)
田沼 順一 鹿児島大学, 歯学部, 助手 (20305139)
富田 浩一 鹿児島大学, 歯学部, 助手 (20315427)
|
|---|
| Project Period (FY) |
1999 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1999: ¥10,100,000 (Direct Cost: ¥10,100,000)
|
|---|
| Keywords | Dark-Agouti rats / Wistar / Furth rats / Tongue carcinoma (TC) / Chemical carcinogenesis / Susceptible genes to TC / Resistant genes to TC / Congenic rats / Alleles / ポジショナルクローニング / 4NQO誘発癌 |
|---|
| Research Abstract |
We have recently reported that the susceptibility of rats to tongue cancers (TCs) induced by 4-nitroquinoline 1-oxide (4NQO) substantially varies depending on the genetic background of organisms. In present study, to map NQO1 encoding the catalytic enzyme of 4NQO, we have investigated DNA sequence analysis of NQO1 in rats for detecting polymorphisms of NQO1. As a result, we have found genetic difference of NQO1 between DA and WF strains. This genetic difference has characterized single nucleotide polymorphisms (SNPs) which shows a C in DA rat and a T in WF rat at nucleotide position 121, which exists in the 5'-flanking region of NQO1 structural gene. Furthermore, by PCR-RFLP analysis utilizing this SNPs, we also have found that NQO1 in rats locates on about 17-cM between D19Rat15 and D19Rat90 on Chr 19. In addition, we also have revealed that DNA sequence at intron 3 of NQO1 is 280-bp longer in DA and WF strains than that of NQO1 known generally. Our study may make it possible to pinpoint the candidate genes for particular diseases, and play an important role in throwing light on the mechanism not only of the experiments of the rat models, but also human diseases, by referring to the syntenic regions of the mouse and human chromosomes.
|
