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Maps of susceptible and/or resistant genes to chemically induced tongue carcinomas using the rat derived from a speed congenic strain originating from the Dark-Agouti and Wistar/Furth progenitors

Research Project

Project/Area Number 11470399
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionKagoshima University

Principal Investigator

KITANO Motoo  Kagoshima University Dental School, Professor, 歯学部, 教授 (10142118)

Co-Investigator(Kenkyū-buntansha) HIRAYAMA Yoshikazu  Kagoshima University Dental School, Research Associate, 歯学部, 助手
SEMBA Ichiro  Kagoshima University Dental School, Associate Professor, 歯学部, 助教授 (60145505)
五味 暁憲  鹿児島大学, 歯学部, 助手 (10325798)
田沼 順一  鹿児島大学, 歯学部, 助手 (20305139)
富田 浩一  鹿児島大学, 歯学部, 助手 (20315427)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1999: ¥10,100,000 (Direct Cost: ¥10,100,000)
KeywordsDark-Agouti rats / Wistar / Furth rats / Tongue carcinoma (TC) / Chemical carcinogenesis / Susceptible genes to TC / Resistant genes to TC / Congenic rats / Alleles / ポジショナルクローニング / 4NQO誘発癌
Research Abstract

We have recently reported that the susceptibility of rats to tongue cancers (TCs) induced by 4-nitroquinoline 1-oxide (4NQO) substantially varies depending on the genetic background of organisms. In present study, to map NQO1 encoding the catalytic enzyme of 4NQO, we have investigated DNA sequence analysis of NQO1 in rats for detecting polymorphisms of NQO1. As a result, we have found genetic difference of NQO1 between DA and WF strains. This genetic difference has characterized single nucleotide polymorphisms (SNPs) which shows a C in DA rat and a T in WF rat at nucleotide position 121, which exists in the 5'-flanking region of NQO1 structural gene. Furthermore, by PCR-RFLP analysis utilizing this SNPs, we also have found that NQO1 in rats locates on about 17-cM between D19Rat15 and D19Rat90 on Chr 19. In addition, we also have revealed that DNA sequence at intron 3 of NQO1 is 280-bp longer in DA and WF strains than that of NQO1 known generally. Our study may make it possible to pinpoint the candidate genes for particular diseases, and play an important role in throwing light on the mechanism not only of the experiments of the rat models, but also human diseases, by referring to the syntenic regions of the mouse and human chromosomes.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report

Research Products

(37 results)

All Other

All Publications (37 results)

  • [Publications] Semba I, Funakoshi K, Kitano M: "Histomorphometric analysis of age changes in the human inferior alveolar artery"Arch Oral Biol. 46. 13-21 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanbara K, Tanuma J, Gotoh K, Kitano M, Nakashima H, et al.: "Biological and genetic characterization of a human immunodeficiency virus strain resistant to CXCR4 antagonist T134"AIDS Res Human Restrovir. 17. 615-622 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanuma J, Fujii K, Shisa H, Hiai H, Kitano M, et al.: "Five quantitative trait loci affecting 4-nitroquinoline 1-oxide-induced tongue cancer in the rat"Jpn J Cancer Res. 92. 610-616 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitano M, Taya Y, Mohri M, Kawashima M: "Dysbaric osteonecrosis (DON) in the femur of an experimental dog : pathological aspects"Kagoshima Univ Res Cnt Pacific Islands, Occasional Papers No. 34. 34. 177-183 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe N, Okochi E, Hirayama Y, Ushijima T, et al.: "Single nucleotide instability without microsatellite instability in rat mammary carcinomas"Cancer Res. 61. 2632-2640 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano M, Tanuma J, Tsuneyoshi M, Kitano M, et al.: "Solitary fibrous tumor in the mental region"Pathol Intern. 51. 905-908 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasui K, Saiga H, Wang Y, Zhang PJ, Semba I: "Early expressed genes showing a dichotomous developing pattern in the lancelet embryo"Dev Growth Differ. 43. 185-194 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitano M, Hirano M, Ohno M, Hattori S, et al.: "Muscle necrosis and regeneration with lack of marked hemorrhage induced in the rat after envenomation of Trimeresurus flavoviridis venom and its components, phospholipase A2 isozymes"South Pacific Study. 22. 31-39 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshikazu Hirayama, Toshikazu Ushijima, Takashi Kuramoto, Motoo Kitano, Takashi Sugimura and Minako Nagao: "Linkage mapping of the rat Msh2 DNA mismatch repair gene on chromosome 6"Exp. Anim.. 48. 63-34 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshikazu Hirayama, Kuniko Wakazono, Masami Yamamoto, Mjotoo Kitano, Masae Tatematsu, Minako Nagao, Takashi Sugimura and Toshikazu Ushijima: "Rare mutations of p53, Ki-ras, and β-catenin genes and absence of K-sam and c-erbB-2 amplification in N-methyl-N-nitro-N-nitorsoguanidine-induced rat stomach cancers"Mol. Carcinogen.. 125. 42-47 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jun-ichi Tanuma, Motoo Kitano, Hayase Shisa and Hiroshi Hiai: "Polygenic susceptibility and resistance to 4-nitroquinoline 1-oxide-injduced tongue carcinogenesis in the rat"J. Exp. Anim. Sci.. 41. 68-77 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Gabriel Landini, Yoshikazu Hirayama, Tie-Jun Li and Motoo Kitano: "Increased fractal complexity of the epithelial-connective tissue interface in the tongue of 4NQO-treated rats"Pathol. Res. Pract.. 196. 251-258 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Motoo Kitano: "Host genes controlling the susceptibility and resistance to squamous cell carcinoma of the tongue in a rat model"Pathology Intern.. 50. 353-362 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Motoo Kitano, Yoshikazu Hirayama, Jun-ichi Tanuma, Tie-Jun Li, Masato Hirano, Koichi Tomita and Ichiro Semba: "Mutation of p53 gene during 4NQO-induced tongue carcinogenesis in the Dark-Agouti rat"Reports of Kagoshima University Project : Interactive Studies on Food, Health and Environmental Sciences in Kagoshima. No.3. 260-269 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichiro Semba, Tetsuro Ishigami, Kazumasa Sugihara and Motoo Kitano: "Higher osteoclastic demineralization and highly mineralized cement lines with osteocalcin deposition in a mandibular cortical bone of autosomal dominant osteopetrosis Type II : ultrastructural and undecalcified histological investigations"Bone. 27. 389-395 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jun-ichi Tanuma, Kei Fujii, Masato Hirano, Hiroshi Matsuuchi, Hayase Shisa, Hiroshi Hiai and Motoo Kitano: "Five quantitative trait loci affecting 4-nitroquinoline 1-oxide-induced tongue cancer in the rat"Jpn J Cancer Res. 92. 610-616 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kenji Kanbara, Setsuko Sato, Jun-ichi Tanuma, Hirokazu Tamamura, Kazuyo Gotoh, Manabu Yoshimori, Taisei Kana-moto, Motoo Kitano, Nobutaka Fujii and Hideki Nakashima: "Biological and genetic characterization of a human immunodeficiency virus strain resistant to CXCR4 antagonist T134"AIDS Res Hum Restrov. 17. 615-622 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masato Hirano, Jun-ichi Tanuma, Toru Shimoda, Kazumasa Sugihara, Masazumi Tsuneyoshi and Motoo Kitano: "Solitary fibrous tumor in the mental region"Pathol Int. 51. 905-908 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Motoo Kitano, Masato Hirano, Makoto Yahata, Eri Umemura, Aichi Yoshida A, Shosaku Hattori, Naoko Ueda, Daisuke Tsuru and Motonori Ohno: "Muscle necrosis and regeneration with lack of marked hemorrhage induced in the rat after envenomation of Trimeresurus flavoviridis venom and its components, phospholipase A2 isozyme"South Pacific Study. 22. 31-39 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Semba I, Funakoshi K, Kitano M: "Histomorphometric analysis of age changes in the human inferior alveolar artery"Arch Oral Biol. 46. 13-21 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanbara K, Tanuma J, Gotoh K, Kitano M, Nakashima H, et al.: "Biological and genetic characterization of a human immunodeficiency virus strain resistant to CXCR4 antagonist T134"AIDS Res Human Restrovir. 17. 615-622 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanuma J, Kujii K, Shisa H, Hiai H, Kitano M, et al.: "Five quantitative trait loci affecting 4-nitroquinoline 1-oxide-induced tongue cancer in the rat"Jpn J Cancer Res. 92. 610-616 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kitano M, Taya Y, Mohri M, Kawashima M: "Dysbaric osteonecrosis (DON) in the femur of an experimental dog : pathological aspects"Kagoshima Univ Res Cnt Pacific Islands, Occasional Papers No.34. 34. 177-183 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watanabe N, Okochi E, Hirayama Y, Ushijima T, et al.: "Shingle nucleotide instability without microsatellite instability in rat mammary carcinomas"Cancer Res. 61. 2632-2640 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirano M, Tanuma J, Tsuneyoshi M, Kitano M, et al.: "Solitary fibrous tumor in the mental region"Pathol Intern. 51. 905-908 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasui K, Saiga H, Wang Y, Zhang PJ, Semba I: "Early expressed genes showing a dichotomous developing pattern in the lancelet embryo"Dev Growth Differ. 43. 185-194 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kitano M, Hirano M, Ohno M, Hattori S, et al.: "Muscle necrosis and regeneration with lack of marked hemorrhage induced in the rat after envenomation of Trimeresurus flavo viridis venom and its components, phospholipase A2 isozymes"South Pacific Study. 22. 31-39 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirayama Y,Kitano M,Nagao M,Sugimura T,Ushijima T, et al: "Rare mutations of p53, Ki-ras, and β-catenin genes and absence of K-sam and cerbB-2 amplification in MNNG-induced rat stomach cancers"Mol Carcinogenesis. 25(1). 42-47 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tanuma J,Kitano M,Shisa H,Hiai H.: "Polygenetic susceptibility and resistance to 4-nitroquinoline 1-oxide-induced tongue carcinomas in the rat"J Exp Animal Sci. 41(1). 68-77 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitano M,Tanuma J,Hirayama Y,Li T-J,Hirano M,Tomita I,Semba I.: "Green tea and rat tongue carcinogenesis (2): mutation of p53 gene during 4NQO-induced carcinogenesis in the Dark-Agouti rat"Reports of Kagoshima University Project : Interactive Studies on Food, Health and Environmental Sciences in Kagoshima. No.3. 137-141 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitano M.: "Host genes controlling the susceptibility and resistance to squamous cell carcinoma in a rat model"Pathol Intern. 50(5). 353-362 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Landini G,Hirayama Y,Li T-J,Kitano M.: "Increased fractal complexity of the epithelial-connective tissue interface in the tongue of 4NQO-treated rats"Pathol Res Pract. 196(4). 251-258 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Semba I,Ishigami T,Sugihara K,Kitano M: "Higher osteoclastic demineralization and highly mineralized cement lines with osteocalcin deposition in a mandibular cortical bone of autosomal dominant osteoptrosis type II : ultrastructural and undecalcified histological investigations"Bone. 27(3). 389-395 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitano M,Tanuma J,Hirayama Y,Hirano M,Semba I..: "Green tea and rat tongue carcinogenesis: establishment of a rat model of carcinogenesis"Reports of Kagoshima University Project: Interactive Studies on Food,Health and Environmental Sciences in Kagoshima. No.2. 137-141 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanuma J,Kitano M,Shisa H,Hiai H.: "Polygenetic susceptibility to 4-nitroquinoline 1-oxide-induced tongue carcinogenesis in the rat"J Exp Animal Sci. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitano M,Tanuma J,Hirayama Y,LiT-J,Hirano M,Tomita I,Semba I..: "Green tea and rat tongue carcinogenesis(2): mutation of p53 gene during 4NQO-induced carcinogenesis in the Dark-Agouti rat"Reports of Kagoshima University Project: Interactive Studies on Food,Health and Environmental Sciences in Kagoshima. No.3(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitano M.: "Host genes controlling the susceptibility and resistance to squamous cell carcinoma in a rat model"Pathol Intern. 49(印刷中). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-03-31   Modified: 2016-04-21  

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