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Analysis of bacterial biofilms as a virulence factor for refractory periapical periodontitis

Research Project

Project/Area Number 11470404
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

EBISU Shigeyuki  Osaka University, Graduate School of Dentistry, Professor., 大学院・歯学研究科, 教授 (50116000)

Co-Investigator(Kenkyū-buntansha) NOIRI Yuichiro  Osaka University, Graduate School of Dentistry, Research Associate, 大学院・歯学研究科, 助手 (50218286)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 2000: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1999: ¥7,500,000 (Direct Cost: ¥7,500,000)
Keywordsrefractory periapical periodontitis / bacterial biofilm / Porphyromonas gingivalis / glycocalyx / Modified Robbins device / planktonic bacteria / antibiotics / ATP assay / 根尖孔外 / バイオフィルム形成細菌 / 走査型電子顕微鏡
Research Abstract

We morphologically examined the clinical specimens obtained from extracted teeth with refractory periapical periodontitis, canal filling materials excessively filled, and apical tooth fragment. In 9 of 11 samples, bacterial biofilms covered with glycocalyx-like structure were detected at the extraradicular area of canal filling materials and periapical root surface in electron microscopic observation. Filaments, long rods and spirohete-shaped bacteria were predominant in the biofilms at extraradicular sites of canal filling materials and planktonic cells emigrated from the glycocalyx-like structure, were also detected in several sites.
Next, we generated the Porphyromonas gingivalis biofilms by use of modified Robbins device (MRD), and we investigated the biofilms morphologically as well as biological activity compared to their planktonic counterparts, using ATP assay based on luminescence generated by luciferase-luciferin interaction. The rate of increase of intracellular ATP concentra … More tion of the planktonic bacteria at log phase was 13.88 nmol/h, while that of mature biofilms after 14 days were 5.05 X 10^<-2> pmol/h, and these results indicated that growth rate of P.gingivalis in mature biofilms were about 1/3.0 X 10^5 of their planktonic counterparts. Formation of biofilms was observed 7 days after circulation in MRD and there was co-relation with the morphological changes of biofilms and increase of intracellular ATP concentration.
To investigate antimicrobial susceptibility of P.gingivalis biofilms, we examined the effects of antimicrobial agents such as chlorhexidine, minocycline-hydrochloride and metronidazole against P.gingivalis biofilms, using ATP assay. ATP concentration was decreased to 1/1000 after incubation with chlorhexidine for a day, and thereafter ATP concentration were gradually decreased. In contrast, ATP concentration after 7 days incubation with minocycline-hydrochloride and metronidazole, were about a half of that of control. These results indicate that minocycline-hydrochloride and metronidazole have no significant bactericidal effects against P.gingivalis formed biofilms. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Noiri Y. et al: "Identification of the periodontal disease-assiciated bacteria in the "plaque-free zone"."Journal of Periodontology. 71. 1319-1326 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 池澤由美子 ら: "Porphyromonas gingivalisバイオフィルムに対する化学療法剤の抗菌効果"日本歯周病学会会誌. 42. 160 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 恵比須繁之 ら: "バイオフィルム感染症としての歯周病の特徴"炎症・再生. 21. 141-147 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 江原篤 ら: "歯性病巣感染の復権と根尖性歯周炎"歯界展望. 97. 414-415 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 野杁由一郎 ら: "根尖性歯周組織疾患の難治化・慢性化に伴う細菌バイオフィルムの関与"Bacterial adherence研究会講演録. 15(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Noiri Y. et al: "Participation of bacterial biofilm in refractry and chronic periapical periodontitis."Journal of Endodontics. 27(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Noiri Y.et al.: "Identification of the periodontal disease-associated bacteria in the "plaque-free zone"."J periodontol. 71. 1319-1326 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikezawa Y.et al.: "Antimicrobial effect of chemotherapeutic agents against Porphyromonas gingivalis biofilms"J Jpn Periodontol. 43 (Special Issue). 160 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ebisu S.et al.: "The characteristics of periodontal disease in respect to biofilm disease."Jpn J Inflamm. 21. 141-147 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ehara A.et al.: "Revival of odontogenic focal infection and periapical periodontitis."Dental outlook. 97. 414-415 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Noiri Y.et al.: "Relevance of bacterial biofilm to refractry and chronic periapical tissue disease."J Soc Bact Adhe. 15 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Noiri Y.et al.: "Participation of bacterial biofilm in refractry and chronic periapical periodontitis."J Endodon. 27 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Noiri Y. et al: "Identification of the periodontal disease-assiciated bacteria in the "plaque-free zone"."Journal of Periodontology . 71・8. 1319-1326 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 池澤由美子 ら: "Porphyromonas gingivalisバイオフィルムに対する化学療法剤の抗菌効果"日本歯周病学会会誌. 42秋季特別号. 160 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 恵比須繁之 ら: "バイオフィルム感染症としての歯周病の特徴"日本炎症学会雑誌. 21・2(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 野杁由一郎 ら: "根尖性歯周組織疾患の難治化・慢性化に伴う細菌バイオフィルムの関与"Bacterial adherence研究会講演録. 15(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Noiri Y. et al: "Participation of bacterial biofilm in refractry and chronic periapical periodontitis."Journal of Endodontics. 27(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 野杁由一郎: "根尖性歯周炎の難治化・慢性化に伴う細菌バイオフィルムの関与"日本歯科保存雑誌. 42春季特別号. 40 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Noiri Y and Ebisu S: "Identification of the periodontal disease-associated bacteria in the "plaque-fee zone""Journal of Periodontology. 71(in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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