| Co-Investigator(Kenkyū-buntansha) |
TERANO Motohiro NAGASAKI UNIVERSITY、SCHOOL OF DENTISTRY, RESEATRCH ASSOCIATE, 歯学部, 助手 (90264259)
KANAOKA Rika NAGASAKI UNIVERSITY、SCHOOL OF DENTISTRY, RESEATRCH ASSOCIATE, 歯学部, 助手 (50336179)
MINAMI Jirou NAGASAKI UNIVERSITY、SCHOOL OF DENTISTRY, RESEATRCH ASSOCIATE, 歯学部, 助手 (80304955)
KOIKE Mari NAGASAKI UNIVERSITY、THE UNIVERSITY HOSPITAL OF DENTISTRY, RESEATRCH ASSOCIATE, 歯学部・附属病院, 助手 (00234667)
NAKAMURA Sigeru NAGASAKI UNIVERSITY、THE UNIVERSITY HOSPITAL OF DENTISTRY, RESEATRCH ASSOCIATE, 歯学部・附属病院, 助手 (90227900)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥8,900,000 (Direct Cost: ¥8,900,000)
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| Research Abstract |
Metal ions released from metal of dental prosthesis and cosmetic and household products induce metal allergy. We suppose that the formation of soluble metal ions(correosion) in the mouth is affected by salivary characteristic. The first aims of this study was to determine concentration of organic acid in whole saliva , and* the second was to analyze containing metal element in cosmetic and household products from clinical viewpoint in metal allergy.
Using EDXRF, containing metal elements in 81 different cosmetic and in 50 household products were analyzed. Resting and stimulated whole saliva were collected from 29 metal allergy patient and 44 dental patients who were free from any symptoms of metal allergy llfcitrol). Stimulated saliva induced by the chewing of paraffin pellet was collected by absorption onto cotton rolls for 5. Resting saliva samples were centrifuged at 3000 rpm for 15 min, and the resulting supernatants were frozen at -20℃ until used. Stimulate saliva samples were also frozen at -20℃. Salivary organic acids.that were phosphoric acid, citric acid, pyruvic acid, malic acid, succinic acid, lactic acid, formic acid, acetic acid, propionic acid and carbonic acid, were quantitatively determined by high-performance liquid chromatography method. Concentration of organic acid in whole saliva are statistically analyzed using Mann-Whitney U test.
1. In mostofcosmetic and household products, Al, Si, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Rb, Sr, Zr, Ba, Bi, and so on were clearly contained.
2. For resting saliva metal allergy patient group had statistically significant higher concentration of pyruvic acid and carbonic acid than control group. While for stimulated saliva metal allergy patient group had statistically significant lower concentration of citric acid, acetic acid and propionic acid than control group.
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