| Project/Area Number |
11470433
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B).
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
MIZUTANI Hideki School of Medicine, Nagoya University, Associate Professor, 医学部, 助教授 (30167663)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HATA Kenichiro School of Medicine, Nagoya University, Associate Professor, 医学部, 助教授 (80293710)
KAGAMI Hideaki School of Medicine, Nagoya University, Assistant Professor, 医学部, 講師 (80242866)
UEDA Minoru School of Medicine, Nagoya University, Professor, 医学部, 教授 (00151803)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥4,900,000 (Direct Cost: ¥4,900,000)
|
|---|
| Keywords | temporomandibular joint / adhesion / culture / electron microscopy / fluorescence staining / actin |
|---|
| Research Abstract |
To characterize the adhesions in internal derangement (ID) of the temporomandibular joint (TMJ), we used a gel contraction model to investigate the ability of fibroblast-like cells derived from human normal articular disc (D), synovium (S) and two types of adhesion tissue of ID in the TMJ (band-like adhesion [BA], pseudowall-like adhesion [PW]).
Sequential measurements of gel diameter for 12 days showed that six kinds of cells contracted CG continuously. Among TMJ-related cells, PW had the greatest contraction potency. Differences in ability of the contraction potency may reflect their proliferative potential, migratory activity, production of and response to cytokines, and synthesis of extracellular matrix products. The order of contraction potency is ; NS>PW>BA>D/GF>S
Arrangement of cells mtd ECM components were examined in two types of adhesions by TEM, SEM and rhodamine-labeled phalloidine staining. In BA, there were a few fibroblast-like cells with thick filopodia processes and orde
… More
rly arranged collagen bundles were prominent. The collagen bundles ran parallel to direction in which the adhesion was extended. In PW, there were a lot of active fibroblast-like cells with many fine filopodia processes and randomly arranged collagen bundles were mainly seen. A part of pseudowall-like adhesion, calcified collagen fibrils were observed in the extracellular matrix. The adhesions that showed the highest resistance during arthroscopic biopsy consisted of calcifiecl collagen fibrils. This structure in internal derangement may strongly inhibit movement of the disc and joint.
In addition the effects of tranilast, N-(3,4-dimethoxycinnamoyl) anthranilic acid, a widely used antiallergy drug in Japan on the gel contraction process was also investigated. The degree of inhibition induced by tranilast was not equal in the two types of fibroblast-like cell ; BA showing a much greater response to tranilast than PW.The reasons for the difference in magnitude of response to tranilast are unclear, although it may reflect differences in the level of the receptor expression for tranilast or response to cytokines by each cell type. Less
|
