Antitumor effect and mechanism of tumor antigen peptide
| Project/Area Number |
11470434
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
AMINO Kayoko Graduate school of Dentistry Osaka University Research Associate, 大学院・歯学研究科, 助手 (50202700)
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| Co-Investigator(Kenkyū-buntansha) |
IWAI Souichi Dental hospital Osaka University Senior resident, 歯学部・附属病院, 医員
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Tumor antigen peptide / adjuvant (MDP) / CTL activity / cytokine (IL-2) |
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| Research Abstract |
First of all we investigated antitumor effect was increased due to tumor antigen peptide (P1A) and Muramyl dipeptide (MDP). We inoculated tumor aotigen peptide (P1A) and Muramyl dipeptide (MDP) after transplanted P815 mastocytoma to mice.
We observed survival rate of those mice. And then we investigated cytotoxic T lymphocytes (CTL) activity, Interleukin 2 (IL-2) production, Interferon γ (IFNγ) production of T lymphocytes of spleen cells.
Next we inoculated P1A, MDP and IL-2 after transplanted P815 mastocytoma to mice. Just then those mice inoculated P1A, MDP and IL-2 survived longer those mice inoculated P1A and MDP.IL-2 was inoculated for 5 days from the second day transplantation of P815 mastocytoma. And those mice showed higher CTL activity and higher IFNγ production. But when IL-2 was inoculated for 5 daya from the 14^<th> day transplantation of P815 mastocytoma, the mice did not survived longer the mice inoculated P1A and MDP.
When the mice inoculated with IL-2, IL-2 receptor of spleen cells were increased.
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Report
(3 results)
Research Products
(12 results)
