Analysis of Masticatory Neural Network using Calcium Imaging
| Project/Area Number |
11470435
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
KOGO Mikihiko Osaka University Graduate School of Dentistry Assoc. Prof, 大学院・歯学研究科, 助教授 (20205371)
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| Co-Investigator(Kenkyū-buntansha) |
HAMAGUTI Munehiro Osaka University Graduate School of Dentistry Research Associate, 大学院・歯学研究科, 助手 (50248019)
LIDA Seiji Osaka University Dental Hospital Assist Prof., 歯学部・附属病院, 講師 (40283791)
MATSUYA Tokuzo Osaka University Graduate School of Dentistry Prof., 大学院・歯学研究科, 教授 (40028759)
YASUI Yasunobu Osaka University Graduate School of Dentistry Research Associate, 大学院・歯学研究科, 助手 (80311760)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥13,300,000 (Direct Cost: ¥13,300,000)
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| Keywords | Trigeminal Nerve / Rhythm / Movement / Control / Pattern / Neurophysiology / Brainstem / Calcium |
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| Research Abstract |
We used the neonatal rats. We analyze the activities of interneurons during rhythmical trigeminal activities in an isolated brainstem block preparation in vitro, using the calcium imaging. For dyeing, we had used Fluo-3. We could not get any results. So we substituted the calcium green for the fluo-3. We recognized the rhythmical activities in the interneuronal membrane potentials in the trigeminal motor nucleus. We also test about the swallowing. We stimulate the solitary tract and nucleus pharmacologicaly and electrically. We could induce the swallowing-like activities in an isolated brainstem block preparation in vitro. This preparation is very useful for analyzing the orofacial functional activities.
We labeled the commissural interneurons with the calcium green. Because the left and right synchronization is usually recognized in this preparation. The previous paper had suggested that the number of interneurons in the trigeminal motor nucleus.
However we could see the many interneuron s in the trigeminal motor nucleus. Some of these showed the rhythmical activities in the calcium imaging. The machine which we used in this study is very useful for analyze the neural networks. However the speed is too slow to analyze the rapid change of the membrane potentials. We have to improve this point in future experiments. We will continue to analyze the interneuronal activities in the trigeminal motor nucleus and to search the central pattern generators for trigeminal rhythmical activities.
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Report
(3 results)
Research Products
(1 results)
