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ESTABLISHMENT AND APPLIANCE OF NEWLY GENE THERAPY FOR DEGENERATIVE ARTHROSIS USING DECOY STRATEGY

Research Project

Project/Area Number 11470440
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

TAKENOSHITA Yasuharu  KYUSHU UNIVERSITY GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROF, 歯学研究院, 助教授 (50117157)

Co-Investigator(Kenkyū-buntansha) KUBOTA Yasutaka  KYUSHU UNIVERSITY GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROF, 歯学研究院, 助手 (60205151)
NAKAGAWA Kazunori  KYUSHU UNIVERSITY GRADUATE SCHOOL OF MEDICAL SCIENCE, ASS PROF, 医学研究院, 講師 (50217668)
ISHIBASHI Hiroaki  KYUSHU UNIVERSITY GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROF, 歯学研究院, 助手 (90254630)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥12,100,000 (Direct Cost: ¥12,100,000)
KeywordsTEMPOROMANDIBULAR JOINT / EXTRACELLULAR MATRIX / GENE THERAPY
Research Abstract

(l) Examination for the expression of uPA and MMPs stimulated by several cytokines using cultured cells.
The expression of uPA and MMPs was increased by the stimulation with TNFα using cultured cells derived from cartilaginous tissue of TMJ.
(2) Examination of transcriptional factor related to cytokines stimulation. Transcriptional factors, NF-kB and Sp1, were activated by TNFα stimulation at the cultured cells derived from cartilaginous tissue.
(3) Establishment of the condition for the gene transfer
We established the condition for the Decoy oligodeoxynucletides targeted to NF-kB and Sp1 using HVJ-liposome method, and decoy ODNs were routinely transferred to 1OO % of the cells.
(4) Inhibition of the expression of uPA and MMPs by Decoy ODNs transfection
The expression of the uPA and MMPs were inhibited by the transfection of Decoy ODNs to the cultured cells derived from cartilaginous tissue.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Ishibashi H, et al.: "Sp1 Decoy Transfected Cells Suppresses the Expression of VEGF, TGFβ1, and Tissue Facotr and also Cell Grwoth and Invasion Activites."Cancer Research. 60. 6531-6536 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishibashi H, et al.: "Hypoxia-Induced Angiogenesis of Cultured Human Salivary Gland Carcinoma Cells Involves Enhancement VEGF Production and bFGF Release."Oral Oncology. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakagawa K, et al.: "Angiogenesis and Its Regulation ; Role of VEGF."Semin Thromb Hemost.. 143. 201-206 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kubota Y, et al.: "Interleukin-1α-dependent Regulation of Matrixiz Matalloproteinase-9 (MMP-9) Secretion and Activation in the Epithelial Cells of Odontogenic Jaw Cysts."Journal of Dental Research. 79(6). 1423-1430 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Ishibashi, et al: "Sp1 Decoy Transfected Cells Suppresses the Expression of VEGF, TGFβ1, and Tissue Facotr and also Cell Grwoth and Invasion Activites."Cancer Research. 60. 6531-6536 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Ishibashi, et al: "Hypoxia-Induced Angiogenesis of Cultured Human Salivary Gland Carcinoma Cells Involves Enhancement VEGF Production and bFGF R elease."Oral Oncology. 37. 77-83 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Kubota, et al: "Interleukin-1 α-dependent Regulation of Matrix Metalloproteinase-9 (MMP-9) Secretion and Activation in the Epithelial Cells of Odontogenic Jaw Cysts."Journal of Dental Research. 79 (6). 1423-1430 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishibashi H, et al.: "Sp1 Decoy Transfected Cells Suppresses the Expression of VEGF, TGF β1, and Tissue Facotr and also Cell Grwoth and Invasion Activites."Cancer Research. 60. 6531-6536 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishibashi H, et al.: "Hypoxia-Induced Angiogenesis of Cultured Human Salivary Gland Carcinoma Cells Involves Enhancement VEGF Production and bFGF Release."Oral Oncology. (発表予定).

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakagawa K, et al.: "Angiogenesis and Its Regulation ; Role of VEGF."Semin Thromb Hemost.. 143. 201-206 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kubota Y, et al.: "Interleukin-1 α-dependent Regulation of Matrix Metalloproteinase-9 (MMP-9) Secretion and Activation in the Epithelial Cells of Odontogenic Jaw Cysts."Journal of Dental Research. 79(6). 1423-1430 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakagawa K et al: "Angiogenesis and its regulation"Semin Thromb Hemost. (in press).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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