• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of pathological root resorption of deciduous teeth and establishment for the treatment

Research Project

Project/Area Number 11470445
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OGUCHI Haruhisa  Hokkaido Univ., Grad. School of Dent., Prof., 大学院・歯学研究科, 教授 (30124689)

Co-Investigator(Kenkyū-buntansha) HASEGAWA Tomokazu  Hokkaido Univ., Grad. School of Dent., Inst., 大学院・歯学研究科, 助手 (50274668)
YAWAKA Yasutaka  Hokkaido Univ., Grad. School of Dent., Inst., 大学院・歯学研究科, 助手 (60230603)
KAGA Masayuki  Hokkaido Univ., Grad. School of Dent., Asso. Prof., 大学院・歯学研究科, 助教授 (70125300)
野田 守  北海道大学, 歯学部, 助手 (10301889)
長内 正数  北海道大学, 歯学部・附属病院, 助手 (80292035)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥11,800,000 (Direct Cost: ¥11,800,000)
Keywordshuman deciduous teeth / pathological root resorption / SEM and TEM observation / PCR detection / 16S rDNA / osteoprotegerin / periodontal ligament / osteoclast / 細菌感染 / 歯根膜線維 / 16S rDNA / 破歯細胞 / 分子生物学
Research Abstract

SEM observation of root surface of deciduous teeth surface pathologically resorted was done. Exudates from root cannal were analyzed by PCR detection. Moreover, periodontal ligament cells were cultured to study the moleculars concerned with osteoclast differentiation.
1. By SEM observation of deciduous root with severe periodontitis, numerous bacteriums were observed in the root canals or on the root surfaces. However, no bacterium was there in the dentin tubules of apical region. Pathological root resorption was observed in wide region, but there was no odontoclasts. The pathological root resorption was advanced irregularly in the resion and a lot of resorbed lacunae were observed just like a worm-eaten shape. Therefore, it was thought that the pathological root resorption was greatly affected with bacteriums and their products.
2. To determine the bacterial outcome of apical periodontitis in deciduous teeth, Intra-canal exudates were absorbed with sterilized paper points. The suspensio … More ns with exudates were analyzed by PCR. The result was that bacterial 16s rDNA was detected in 12 cases out of 16cases tested. Bacterial analysis by PCR is effective to clarify the bacterial species and treatment for periodontitis.
3. Periodontal ligament (PDL) cells produce ten-fold osteoprotegerin (OPG) compared with osteoblasts and gingival cells. It suggests that osteoclast differentiation is depressed by OPG from PDL cells. It appears that PDL is not only connective tissue between a tooth and alveolar bone but also protection of bone and teeth resorption from osteoclast. We think that it is possible to depress for pathological root pesorption with canal root drag containing OPG.
These results suggested that complete root canal rinsing and disinfection of root canal are an effective treatment of pathological root resorption. Detection of bacteria species and for the treatment is needed. Moreover, induction of osteoprotegrin was suggested for the new treatment in the apical root inflammation. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yawaka, Y.: "Delayed eruption of premolars with periodontitis of primary predecessors and a cystic lesion : a cese report"International Journal of Paediatric Dentistry. 12. 53-60 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaga, M.: "The in vitro cytotoxicity of eluates from dentin bonding resins and their effect on tyrosine phosphorylation of L929 cells"Dental Materials. 17. 333-339 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaga, M.: "Approach to prevent progression of mesial proximal caries originating in premature first molars"Caries Preventive Restoration. 151-161 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hashimoto, M.: "In vivo degradation of resin-dentin bonds in humans over 1-3years"Journal of Dental Research. 79(6). 1385-1391 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] YAWAKA, Y. (et al.): "Delayed eruption of premolars with periodontitis of primary predecessors and a cystic lesion: a case report"International Journal of Paediatric Dentistry. 12. 53-60 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] KAGA, M. (et al.): "The in vitro cytotoxity of elute from dentin bonding resins and their effect on tyrosine phosphorylation of L929 cells"Dental Materials. 17. 333-339 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] KAGA, M. (et al.): "Approach to prevent progression of mesial proximal caries originating in premature first molars"Caries Preventive Restoration. 151-161 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hashimoto, M. (et al.): "In vivo degradation of resin-dentin bonds in humans over 1-3 years"Journal of Dental Research. 79(6). 1385-1391 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yawaka, Y.: "Delayed eruption of premolars with periodontitis of primary predecessors and a cystic lesion: a cese report"International Journal of Paediatric Dentistry. 12. 53-60 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kaga, M.: "The in vitro cytotoxicity of eluates from dentin bonding resins and their effect on tyrosine phosphorylation of L929 cells"Dental Materials. 17. 333-339 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kaga, M.: "Approach to prevent progression of mesial proximal caries originating in premature first molars"Caries Preventive Restoration. 151-161 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 菊入崇 他6名: "ヒト歯根膜由来細胞におけるNO産生について"小児歯誌. 37・4. 768-774 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi