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Characterization of factors controlling the differentiation of periodontal ligament cells

Research Project

Project/Area Number 11470460
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KASUGAI Shohei  Tokyo Med.& Dent.Univ., Graduate School, Prof., 大学院・医歯学総合研究科, 教授 (70161049)

Co-Investigator(Kenkyū-buntansha) OIDA Shinichiro  Tokyo Med.& Dent.Univ., Graduate School, Assistant Prof., 歯学部, 助教授 (10114745)
ODA Shigeru  Tsurumi Univ., Faculty of Dentistry, Associate Prof., 大学院・医歯学総合研究科, 講師 (70160869)
KURODA Shinji  Tokyo Med.& Dent.Univ., Dental Hospital, Research Associate, 歯学部・附属病院, 助手 (50323689)
飯村 忠浩  東京医科歯科大学, 大学院・医歯学総合研究科, 助手 (20282775)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2000: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1999: ¥9,400,000 (Direct Cost: ¥9,400,000)
KeywordsPeriodontal Tissue / Regeneration / Amelogenin / S100A4 / Calcium Binding Proteins / Mineralization / Mechanical Stress / Bone / 歯根膜 / 分化 / エナメルタンパク
Research Abstract

The purpose of this research was to characterize molecules which control the differentiation and function of periodontal ligament (PDL) cells. We constructed cDNA library of bovine PDL tissue and cloned cDNA of S100A4, one of the calcium binding proteins. We observed that mRNA expression level of S100A4 in PDL of erupted teeth was high and that PDL cells secreted this protein, which localized adjacent to collagen fibers. Addition of recombinant S100A4 protein to oseteoblastic culture inhibited mineralization. In culture, PDL cells under mechanical stress inceased mRNA expression of S100A4 together with cytoskeletal proteins. Although the expression level of this protein in MC3T3-E1 cells (osteoblastic cells) was low, inhibition of S100A4 expression in MC3T3-E1 cells stimulated mineraliztion in culture. These results strongly indicate that S100A4 is an inhibitor of meranlization in PDL tissue. On the other hand, porcine enamel matrix derivative (EMD) stimulated growth and differentiation of bovine PDL cells and Kusa cells (derived from mouse bone marrow), resulting the stimulation of mineralization in these culture. The stimulatory effects on osteoblastic cells could also contribute to periodontal tissue regeneration. It is important to clarify effective molecule (s) in EMD and Kusa cells are useful for this investigation. Porcine recombinant amelogenin stimulated the differentiation of Kusa cells indicating the possibility that amelogenin is the effective molecule.Although further studies are necessary, we could apply recombinant amelogenin to periodontal tissue regeneration.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (54 results)

All Other

All Publications (54 results)

  • [Publications] 春日井昇平: "エナメルタンパクの新しい作用:アメロジェニン様タンパクの細胞分化誘導作用"the Quintessence. 20・3. 182 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 春日井昇平: "組織再生を応用した顎堤増加の可能性について."口腔病学会雑誌. 67. 299-309 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 春日井昇平: "骨組織再生への遺伝子導入法の応用"the Quintessence. 19・11. 186 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Itoh D,Yoneda S,Kuroda S,Kondo H,Ohya K,Ohyama T,Kasugai S: "Production of recombinant porcine amelogenin"Journal of Bone and Mineral Research. 15(suppl.1). S247 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroda S,Kondo H,Yoneda S,Itoh D,Ohya K,Ohyama T,Kasugai S: "Bone increase in rat tibia by local administration of aminoterminally truncated rhFGF4(73-206)"Journal of Bone and Mineral Research. 15(suppl.1). S257 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikeda K,Kuroda S,Oida S,Iimura T,Duarte WR,Ohya K,Ishikawa I,Kasugai S: "rhFGF4 promotes ectopic bone formation induced by rhBMP2"Journal of Bone and Mineral Research. 15(suppl.1). S257 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoneda S,Itoh D,Umezawa A,Kondo H,Kuroda S,Ohya K,Ohyama T,Kasugai S: "Effects of enamel matrix derivative (EMDOGAIN) on bone marrow stromal cells"Journal of Bone and Mineral Research. 15(suppl.1). S345 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR,Shibata T,Takenaga K,Ikeda K,Yamauchi M,Ohya K,Ishikawa I,Kasugai S: "S100A4 is Involved in Ca-induced chemotaxis and motility of osteoblastic cells (MC3T3E1)"Journal of Bone and Mineral Research. 15(suppl.1). S506 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamamoto N,Isobe M,Yamaguchi S,Negishi A,Yoshimasu H,Ohya K,Amagasa T,Kasugai S : "Expansion of human bone marrow stromal cells with patient's autorogous serum"Journal of Bone and Mineral Research. 15(suppl.1). S509 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 春日井昇平: "Field遺伝子治療に向けた最新の研究から 骨髄ストローマ細胞の臨床応用"the Quintessence. 19・1. 210 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 黒田真司,春日井昇平,近藤尚知,米田哲,伊藤大輔,大谷啓一,大山喬史: "Amino-terminally truncated rhFGF4の局所投与による骨量増加作用"日本骨代謝学会雑誌. 18(抄録号). 211 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 山本庸介,春日井昇平,磯部昌継,野地秀彦,新井直也,佐藤豊,堀江大介,青木和広,田村幸彦,大谷啓一,天笠光雄: "患者自己血清によるヒト骨髄細胞の骨芽細胞への増殖・分化"日本骨代謝学会雑誌. 18(抄録号). 292 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR,Kasugai S,Iimura T,Takenaga K,Ikeda K,Ohya K,Ishikawa I: "Role of S100A4 calcium-binding protein in mineralized nodule formation"Journal of Dental Research. 79(Special Issue). 221 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikeda K,Kasugai S,Duarte WR,Kuroda S,Oida S,Iimura T,Ohya K,Ishikawa I: "Expression of FGFs and their receptors during BMP2-induced bone formation"Journal of Dental Research. 79(Special Issue). 218 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoneda S,Kasugai S,Itoh D,Umezawa A,Kondo H,Kuroda S,Ohya K,Ohyama T: ".Effect of enamel matrix derivative (EMDOGAIN) on osteoblastic cells"Journal of Dental Research. 79(Special Issue). 474 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR,Mikuni-Takagaki Y,Kawase T,Iimura T,Oida S,Ohya K,Takenaga K,Ishikawa I.Kasugai S: "Effects of mechnical stress on the mRNA expression of S100A4 and cytoskeletal components by periodontal ligament cells"Journal of Medical and Dental Sciences. 46. 117-122 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroda S.Kasugai S.Oida S.Iimura T.Ohya K.Ohyama T: "Anabolic effect of aminoterminally truncated fibroblast growth factor 4 (FGF4) on bone"Bone. 25・4. 431-437 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR.Iimura T.Oida S.Takenaga K.Ohya K.Ishikawa I.Kasugai S: "Extracellular role of S100A4 calcium-binding protein in the periodontal ligament"Biochemical and Biophysical Research Communications. 255・2. 416-420 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR,池田かおり,石川烈,春日井昇平,大谷啓一,飯村忠浩,大井田新一郎: "歯周組織におけるS100A4カルシウ結合タンパクの発現とその生理的役割"口腔病学会雑誌. 66. 223 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR,Kasugai S,Iimura T,Ikeda K,Takenaga K,Ohya K,Ishikawa I: "Roles of the S100A4 calcium-binding protein in periodontal ligament and osteoblast biology"Proceeding of the Third Symposium on Tissue Engineering for Hard Tissues (Tokyo Medical and Dental University). 39-42 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 米田哲,春日井昇平,近藤尚知,黒田真司,伊藤大輔,梅澤明弘,大谷啓一,大山喬史: "エナメル基質の骨系細胞に対する分化誘導作用"Proceeding of the Third Symposium on Tissue Engineering for Hard Tissues (Tokyo Medical and Dental University). 12-15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kasugai S: "Molecules controlling functions and differentiation of periodontal ligament cells and osteoblasts : S100A4 and enamel matrix proteins"Symposium Abstract Book, The 40th Anniversary Symposium, Department of Periodontology, College of Dentistry, Seoul National University, Korea. 10-13 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroda S,Kasugai S,Kondo H,Yoneda S,Ito D,Mukohyama H,Iimura T,Oida S,Ohya K,Ohyama T: "Increase of femoral trabecular bone mineral density of mice by amino-terminally truncated rhFGF4 and its histomorphometric analysis"Journal of Bone and Mineral Research. 14(suppl.1). SA086 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR,Kasugai S,Takenaga K,Ikeda K,Iimura T,Ohya K,Ishikawa I: "Effects of high extracellular calcium on the expression of the calcium-binding protein S100A4 in osteoblastic MC3T3-E1 cells."Journal of Bone and Mineral Research. 14(suppl.1). F033 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 黒田真司,春日井昇平,大井田新一郎,飯村忠浩,向山仁,近藤尚知,米田哲,伊藤大輔,大谷啓一,大山喬史: "Amino-terminally truncated rhFGF4の作製とその骨量増加作用"日本骨代謝学会雑誌. 17・2. 210 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Itoh D, Yoneda S, Kuroda S, Kondo H, Ohya K, Ohyama T, Kasugai S.: "Production of recombinant porcine amelogenin"Journal of Bone and Mineral Research. 15(suppl.1). S247 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroda S, Kondo H, Yoneda S, Itoh D, Ohya K, Ohyama T, Kasugai S.: "Bone increase in rat tibia by local administration of aminoterminally truncated rhFGF4(73-206)."Journal of Bone and Mineral Research. 15(suppl.1). S257 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikeda K, Kuroda S, Oida S, Iimura T, Duarte WR, Ohya K, Ishikawa I, Kasugai S.: "rhFGF4 promotes ectopic bone formation induced by rhBMP2"Journal of Bone and Mineral Research. 15(suppl.1). S257 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoneda S, Itoh D, Umezawa A, Kondo H, Kuroda S, Ohya K, Ohyama T, Kasugai S.: "Effects of enamel matrix derivative (EMDOGAIN) on bone marrow stromal cells"Journal of Bone and Mineral Research. 15(suppl.1). S345 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR, Shibata T, Takenaga K, Ikeda K, Yamauchi M, Ohya K, Ishikawa I, Kasugai S.: "S100A4 is Involved in Ca-induced chemotaxis and motility of osteoblastic cells (MC3T3E1)."Journal of Bone and Mineral Research. 15(suppl.1). S506 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamamoto N, Isobe M, Yamaguchi S, Negishi A, Yoshimasu H, Ohya K, Amagasa T, Kasugai S.: "Expansion of human bone marrow stromal cells with patient's autorogous serum"Journal of Bone and Mineral Research. 15(supppl.1). S509 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR, Kasugai S, Iimura T, Takenaga K, Ikeda K, Ohya K, Ishikawa I.: "Role of S100A4 calcium-binding protein in mineralized nodule formation."Journal of Dental Research. 79(Special Issue). 221 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikeda K, Kasugai S, Duarte WR, Kuroda S, Oida S, Iimura T, Ohya K, Ishikawa I.: "Expression of FGFs and their receptors during BMP2-induced bone formation."Journal of Dental Research. 79(Special Issue). 218 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoneda S, Kasugai S, Itoh D, Umezawa A, Kondo H, Kuroda S, Ohya K, Ohyama T.: "Effect of enamel matrix derivative (EMDOGAIN) on osteoblastic cells."Journal of Dental Research. 79(Special Issue). 474 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR, Mikuni-Takagaki Y, Kawase T, Iimura T, Oida S, Ohya K, Takenaga K, Ishikawa I.Kasugai S.: "Effects of mechnical stress on the mRNA expression of S100A4 and cytoskeletal components by periodontal ligament cells."Journal of Medical and Dental Sciences. 46. 117-122 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroda S.Kasugai S.Oida S.Iimura T.Ohya K.Ohyama T.: "Anabolic effect of aminoterminally truncated fibroblast growth factor 4 (FGF4) on bone."Bone. 25(4). 431-7 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR.Iimura T.Oida S.Takenaga K.Ohya K.Ishikawa I.Kasugai S.: "Extracellular role of S100A4 calcium-binding protein in the periodontal ligament."Biochemical and Biophysical Research Communications. 255(2). 416-420 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR, Kasugai S, Iimura T, Ikeda K, Takenaga K, Ohya K, Ishikawa I.: "Roles of the S100A4 calcium-binding protein in periodontal ligament and osteoblast biology"Proceeding of the Third Symposium on Tissue Engineering for Hard Tissue (Tokyo Medical and Dental University). 39-42 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kasugai S.: "Molecules controlling functions and differentiation of periodontal ligament cells and osteoblasts : S100A4 and enamel matrix proteins."The 40th Anniversary Symposium, Department of Periodontology, College of Dentistry, Seoul National University, 1999.10.18 Seoul, Korea. Symposium Abstract Book. 10-13

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroda S, Kasugai S, Kondo H, Yoneda S, Ito D, Mukohyama H, Iimura T, Oida S, Ohya K, Ohyama T.: "Increase of femoral trabecular bone mineral density of mice by amino-terminally truncated rhFGF4 and its histomorphometric analysis"Journal of Bone and Mineral Research. 14(suppl.1). SA086 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duarte WR, Kasugai S, Takenaga K, Ikeda K, Iimura T, Ohya K, Ishikawa I.: "Effects of high extracellular calcium on the expression of the calcium-binding protein S100A4 in osteoblastic MC3T3-E1 cell"Journal of Bone and Mineral Research. 14(suppl.1). F033 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Itoh D,Yoneda S,Kuroda S,Kondo H,Ohya K,Ohyama T,Kasugai S: "Production of recombinant porcine amelogenin."Journal of Bone and Mineral Research. 15(suppl.1). S247 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoneda S,Itoh D,Umezawa A,Kondo H,Kuroda S,Ohya K,Ohyama T,Kasugai S.: "Effects of enamel matrix derivative (EMDOGAIN) on bone marrow stromal cells."Journal of Bone and Mineral Research. 15(suppl.1). S345 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Duarte WR,Shibata T,Takenaga K,Ikeda K,Yamauchi M,Ohya K,Ishikawa I,Kasugai S: "S100A4 is lnvolved in Ca-induced chemotaxis and motility of osteoblastic cells (MC3T3E1)."Journal of Bone and Mineral Research. 15(suppl.1). S506 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoneda S,Kasugai S,Itoh D,Umezawa A,Kondo H,Kuroda S,Ohya K,Ohyama T.: "Effect of enamel matrix derivative (EMDOGAIN) on osteoblastic cells."Journal of Dental Research. 79(Special Issue). 474 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Duarte WR,Kasugai S,Iimura T,Takenaga K,Ikeda K,Ohya K,Ishikawa I.: "Role of S100A4 calcium-binding protein in mineralized nodule formation."Journal of Dental Research. 79(Special Issue). 221 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ikeda K et al.: "Expression of FGFs and their receptors during BMP2-induced bone formation"Journal of Dental Research. 79(Special Issue)(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoneda T et al.: "Effect of enamel matrix derivative (EMDOGAIN) on osteoblastic cells"Journal of Dental Research. 79(Special Issue)(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Duarte WR et al.: "Role of S100A4 calcium-binding protein in mineralized nodule formation"Journal of Dental Research. 79(Special Issue)(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Duarte WR et al.: "Effects of mechnical stress on the mRNA expression of S100A4 and cytoskeletal components by periodontal ligament cells"Journal of Medical and Dental Sciences. 46. 117-122 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Duarte WR et al.: "Extracellular role of S100A4 calcium-binding protein in the periodontal ligament"Biochemical & Biophysical Research Communications. 255・2. 416-420 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Duarte WR et al: "歯周組織におけるS100A4カルシウム結合タンパクの発現とその生理的役割"口腔病学会雑誌. 66. 223 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Duarte WR et al.: "Effects of high extracellular calcium on the expression of the calcium-binding protein S100A4 in osteoblastic MC3T3-E1 cells"Journal of Bone and Mineral Research. 14(suppl.1). F033 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Duarte WR et al.: "Periodontal ligament cells secrets S100A4 protein, an inhibitor of mineralization"Journal of Dental Research. 78(Special Issue). 463 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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