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Development of Simple Denitrification System With Autotrophic Bacteria for Developing Countries

Research Project

Project/Area Number 11480151
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 環境保全
Research InstitutionOita University

Principal Investigator

HANO Tadashi  Faculty of Engineering, Oita University Professor, 工学部, 教授 (80038067)

Co-Investigator(Kenkyū-buntansha) TAKANASHI Hirokazu  Faculty of Engineering, Oita University Research Associate, 工学部, 助手 (40274740)
HIRATA Makoto  Faculty of Engineering, Oita University Associate Professor, 工学部, 助教授 (20264327)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥4,300,000 (Direct Cost: ¥4,300,000)
KeywordsDeveloping Countries / Simplification / Denitrification / Autotrophic Bacteria / Sulfur / Thiobacuius denitrificans
Research Abstract

A simple denitrification process suitable for developing countries was proposed by employing autotrophic Thiobacillus dentrificans. The effect of nitrate concentration, phosphorus concentration, alkalinity and inhibitants on the rate of denitrification was investigated with a column packed with sulfur and limestone particles involving denitrification bactaeria, and the following results were obtained.
1) The denitrification proceeded without substrate inhibition up to 1,000 g-nitrate-N/m^3, which demonstrated that most of the wastewater cold be treated without dilution.
2) The minimum phosphorus concentration was evaluated under constant pH controlled by borate-buffer showing that 1 mg-P/m^3 was enough for the present process. However, higher phosphorus concentration was necessary when borate buffer was not used. This was because pH at the reaction site, probably the boundary between biofilm and surface of sulfur particles, may drop due to the release of proton as a result of denitrification.
3) The minimum alkalinity was found as 600g-CaCO_3/m^3 for load of 420g-N/(m^3/d). The inhibition with fluoride and Ni was not observed until 90 and 3 g/m^3, respectively.
It was concluded that the present system was suitable for developing countries because it was simple without any special instruments or technical care and it offered stable performance with slight request for phosphorus even in concentrated wastewater.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] 羽野忠,平田誠,高梨啓和: "醤油製造プロセスにおける物質フローの解析と醤油粕の乾留再資源化"環境科学会誌. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirata M.,H.Takanashi,T.Hano et al: "Kinetics of Lactic Acid Extraction with Quaternary Ammonium Salt"Separation Science and Technology. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takanashi H.,M.Hirata,T.Hana, et al: "Method for Measuring Mutagen Formation Potential (MFP) on Chlorination as a New Water Quality Index"Water Research. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hano T.,M.Hirata,H.Takanashi et al: "Preparation of Metal-Silicate Microballons with Liquid Surfactant Membrane"Solvent Extraction for 21st Century (Soc.of Chem.Ind.). (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 高梨啓和 等: "排水の変異原性物質生成能の解析"水環境学会誌. 23. 352-359 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirata M.,H.Takanashi,T.Hano et al: "Contribution of Water in Extraction and Stripping Kinetics of Lactic Acid with Tri-n-Octylmethylammonium Chloride"Solvent Extraction Research and Development, Japan. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 羽野忠,平田誠,高梨啓和: "ゼロエミッション型産業をめざして-産業における廃棄物再資源化の動向-"シーエムシー. 350 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Rudi Nugroho et al.: "Denitrification of industrial wastewater with sulfur and limestone packed column"Proc.Asian Water Quality 2001, Fukouka, Japan. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Rudi Nugroho et al.: "Removal of Nitrogen from Wastewater with Simple Biological Method"Proc.the First Joint China/Japan Chem.Eng.Symp.. Vol.I. 68-73 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Hano et al.: "Nitrogen Removal from Wastewater of Low C/N Ratio with Autotrophic Bacteria"Proc.Regional Symp.Chem.Eng, 1999, Songkhla Thailand. Vol.II. B48-1, B48-6 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hirata et al.: "Extractive Recovery of Boron in Wastewater from Coal Thermal Power Plant"Solvent Extraction for 21st Century (SCI). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Hano et al.: "Preparation of Metal-Silicate Microballons with Liquid Surfactant Membrane"Solvent Extraction for 21st Century (SCI). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takanashi et al.: "Method for Measuring Mutagen Formation Potential (MFP) on Chlorination as a New Water Quality Index"Water Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hironaka et al.: "Kinetics of Lactic Acid Extraction with Quaternary Ammonium Salt"Separation Science and Technology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hironaka et al.: "Contribution of Water in Extraction and Stripping Kinetics of Lactic Acid with tri-n-octylmethyl ammonium Chloride"Solvent Extraction Research and Development, Japan. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Hano et al.: "Preparation of Metal-Silicate Microballons with Liquid Surfactant Membrane"Solvent Extraction for 21st Century (SCI). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Matsumoto et al.: "Extraction Rates of Amino Acids by an Emulsion Liquid Membrane with tri-n-octylmethylammonium Chloride"J.Chem.Technol.Biotech.. 13. 237-242 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Tong et al.: "Bck Extraction of Lactic Acid with Microporous Hollow Fiber Membrane"J.of Membrane Science. 157. 189-198 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 羽野忠,平田誠,高梨啓和: "醤油製造プロセスにおける物質フローの解析と醤油粕の乾留再資源化"環境科学会誌. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirata M.,H.Takanashi,T.Hano et al: "Kinetics of Lactic Acid Extraction with Quaternary Ammonium Salt"Separation Science and Technology. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Takanashi H.,M.Hirata,T.Hano, et al: "Method for Measuring Mutagen Formation Potential (MFP) on Chlorination as a New Water Quality Index"Water Research. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hano T.,M.Hirata,H.Takanashi et al: "Preparation of Metal-Silicate Microballons with Liquid Surfactant Membrane"Solvent Extraction for 21st Century (Soc.of Chem.Ind.). (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 高梨啓和 等: "排水の変異原性物質生成能の解析"水環境学会誌. 23. 352-359 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirata M.,H.Takanashi,T.Hano et al: "Contribution of Water in Extraction and Stripping Kinetics of Lactic Acid with Tri-n-Octylmethylammonium Chloride"Solvent Extraction Research and Development, Japan. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 羽野忠,平田誠,高梨啓和: "ゼロエミッション型産業をめざして-産業における廃棄物再資源化の動向-"シーエムシー. 350 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tadashi Hano: "Nitrogen Removal from Wastewater of Low C/N Ratio with Autotrophic Bacteria"Proc.of Regional Symposium on Chemical Engineering 1999,Thailand.. II. B48-1-B48-6 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yanpin Tong,Tadashi Hano: "Back extraction of lactic acid with microporous hollow fiber membrnane."J.Mem.Sci.. 157. 189-198 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tadashi Hano: "ANALYSIS OF NATERIAL FLOWS FOR ZERO-EMISSION IN FOOD INDUSTRIES-CASE STUDY FOR SOY SAUCE PRODUCTION PROCESS-"Proc.of The 8th Asian Pacific Confederation of Chemical Engineering,Seoul.. 2. 809-812 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 羽野 忠: "食品産業におけるゼロエミッション化-醤油製造業におけるマスフロー解析-"化学工業. 50. 96-100 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 板坂直樹、羽野 忠: "水中低濃度リンの除去・回収用吸着剤の開発状況と課題"用水と廃水. 41(3). 195-203 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 羽野 忠: "食品製造プロセスにおけるゼロエミッション化技術"M & E. 1999年3月号. 88-93 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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