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The Fundamental Research on the Prophylaxis of Cyclospora Infectious Disease in Water Supply

Research Project

Project/Area Number 11480154
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境保全
Research InstitutionTohoku Gakuin University

Principal Investigator

ISHIBASHI Yoshinobu  Tohoku Gakuin University, Faculty of Engineering, Professor, 工学部, 教授 (10111246)

Co-Investigator(Kenkyū-buntansha) KUNIKANE Shoichi  National Institute of Public Health, Director, 水道工学部, 部長 (90083740)
OMURA Tatsuo  Tohoku University, Graduate School of Engineering, Professor, 大学院・工学系研究科, 教授 (30111248)
OHGAKI Shinnichiro  The University of Tokyo, Graduate School of Engineering, Professor, 大学院・工学系研究科, 教授 (20005549)
KIMURA Kenji  Maezawa Industries Inc., Managing Director, エンジニアリング事業部, 部長
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsCyclospora / Traveler's diarrhea / Protozoa / Chlorine tolerant / Water supply / 磁気ビーズ回収法
Research Abstract

Cyclospora is the same newly-tisen protozoa as Cryptosporidium. Its infection route is a contaminated tap water and foods and cyclospriasis shows symptoms of water-like diarrhea. Unlike Cryptosporidium a mass outbreak has not occurred in Japan. However, seven cases of cyclospriasis are reported as import infectious disease. There is a possibility that Cyclospora takes a risk of mass outbreak through water supply. Generally, research of Cyclospora is just started and knowledge is not enough. The purpose of this research was carried out to get the fundamental knowledge for cyclospriasis prevention.
As the beginning of the study, information retrieval, detection methods of cyclospora oocyst and infection and multiplication using cancer cells were investigated. It took three years for the last experiment. In the final year, cyclospora was identified as C. cayetanensis using genetic analysis. Existence of Cyclospora and Cryptospridium oocyst and Giarrdia cyst in aquatic environment and wastewater, ultraviolet irradiation for inactivation etc. were examined.
On the other hand, we went to Nepal several times, where disease rate is high, and made an inspection of the state of infection.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 木村意司, 石橋良信他: "サイクロスポーラの検出法"第52回全国水道研究発表会. 632-633 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 平田 強, 木村憲司, 石橋良信他: "原虫基準の検討結果"第4回日本水環境学会シンポジウム講演集. 181-182 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Ono, K.Kimura, Y.Ishibashi et al.: "Cryptosporidium and Cyclospota Associated Diarrhea in Kathmandu, Nepal"Cryptosporidium from Molecules to Disease A Major Imternational Conference on Cryptosporidium. 29 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 木村憲司他: "培養細胞を用いたクリプトスポリジウムの感染力評価に関する検討"日本水処理生物学会誌. 別巻,21. 116 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 石橋良信, 木村憲司他: "サイクロスポーラの紫外線照射による不活化"第53回全国水道研究発表会発表予定. (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 木村憲司, 石橋良信他: "新興感染症の原因となる病原性原虫サイクロスポーラの検出方法"用水と廃水印刷中. 44(4). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kimura K.: "Sterilization of Cryptosporidium"Zosui gijyutu. Vol.26, No.3. 18-26 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kimura K., Ishibashi Y. et al.: "Deteotion Method of Cyclospora"52th Meeting of Water Works Research. 632-633 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata T., Kimura K., Ishibashi Y. et al.: "Criteria of Protozoa"4th Symposium of Japan Society on Water Environment. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ono K., Kimura K., Ishibashi Y. et al.: "Cryptosporidium and Cyclospora Associated Diarrhea in Kathmandu, Nepal"Cryptosporidium from Molecules to Disease, A Major International Conference on Cryptosporidium. 29 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kimura K. et al.: "Evaluation of Infection of Cryptosporidium Using Cell Culture"Japanese Journal of Water Treatment Biology, Another Volume. No.21. 116 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishibashi Y., Kimura K.: "Ultraviolet Irradiation for Inaotivation of Cyclospora"53th Meeting of Water Works Research. (plan for presentation). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kimura K., Ishibashi Y. et al.: "Detection Method of Cyclospora of a Newly-risen Infectious Disease"Water and Wastewater. (in printing). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 木村 憲司, 石橋 良信他: "サイクロスポーラの検出法"第52回全国水道研究発表会. 632-633 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 平田 強, 木村 憲司, 石橋 良信他: "原虫基準の検討結果"第4回日本水環境学会シンポジウム講演集. 181-182 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Ono, K.Kimura, Y.Ishibashi et al.: "Cryptosporidium and Cyclospota Associated Diarrhea in Kathmandu, Nepal"Cryptosporidium from Molecules to Disease A Major Imternational Conference on Cryptosporidium. 29 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 木村 憲司他: "培養細胞を用いたクリプトスポリジウムの感染力評価に関する検討"日本水処理生物学会誌. 別巻,21号. 116 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 石橋 良信, 木村 憲司他: "サイクロスポーラの紫外線照射による不活化"第53回全国水道研究発表会. (発表予定). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 木村 憲司, 石橋 良信他: "新興感染症の原因となる病原性原虫サイクロスポーラの検出方法"用水と廃水. 44(4)(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Hashimoto,T.Hirata and S.Kunikane: "Occurrence of Cryptosporidium oocysts and Glardia cysts in a conventlonal water purification plant."The 10th Health-Related Water Microbiology Symposium,. HRMP-B50. (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 木村憲司,石橋良信 他: "サイクロシポーラの検出法"第52回全国水道研究発表会. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Ono,K.Kimura,Y.Ishibashi et al.: "Cryptosporidium and Cyclospora Associated Diarrhea in Kathmandu, Nepal"Cryptosporidium from Molecules to Disease A Major international Conference on Cryptosporidium. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 国包章一,北澤弘美,眞柄泰基: "水道水源におけるクリプトスポリジウム及びジアルジア実態調査結果の解析"水道協会雑誌. 68(4). 22-31 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 国包章一: "水道におけるクリプトスポリジウム対策"厚生. 54(10). 17-19 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 国包章一,伊藤雅喜: "半回分式試験によるナノ濾過膜の基礎的性能評価"水道協会雑誌. 68(12). 29-41 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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