| Project/Area Number |
11480167
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Oita University (2000)
Kyushu University (1999) |
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Principal Investigator |
ISHIKAWA Yuichi Oita University, Department of Applied Chemistry, Associate Professor, 工学部, 助教授 (30184500)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Takeo Oita University, Department of Applied Chemistry, Associate Professor, 工学部・東京理科大・理学部, 助教授 (80261501)
YOSHIMI Takeshi Oita University, Department of Applied Chemistry, Research Associate, 工学部, 助手 (90284786)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2000: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1999: ¥10,800,000 (Direct Cost: ¥10,800,000)
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| Keywords | Double Phospholipid / Chytochrom c / DNA Replication / Porphyrin / Bilayer membrane / チトクロームc / 遺伝子複製 / 自己複製 / 脂質膜の分裂 / 多重リン酸構造 / カルジオリピン / DnaAタンパク質 |
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| Research Abstract |
Most of biolipids are comprised of a single hydrophilic group and double alkyl chains. Besides these typical lipids, one can find out a four-chained lipid with a double hydrophilic head, cardiolipin (CL). The double structure in CL is apparently redundant for physicochemically stabilizing bilayer membrane itself. However, it has been recently reported hat the double structure can be used as a genetic factor for DnaA protein, the initiator of DNA replication of Escherichia coli. It seems for us to be a key to bridge the two phosphates by a glycerol unit in CL.Through systematically synthesizing various double phosphate lipids, herein we show that cooperative action by the two phosphate groups possessing somewhat hydrophobic property on membrane would play a crucial role for interaction with DnaA protein. For generalizing the role of the double phosphate form in lipid, we also find that the double phosphate lipid has influence over the conformation and activity of Cyt.c, not only on the DnaA protein. In the case of the protein binding, the hydroxy group in the bridge is a key group for activating the enzymes, In this study, we conclude that the double head bridging a glycerol is quite instrumental for the enzyme activation.
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