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SIGNIFICANCE OF THE MULTIPLICITY OF ADENYLYL CYCLASE

Research Project

Project/Area Number 11480181
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionYOKOHAMACITY UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

ISHIKAWA Yoshihiro  Yokohama City University, 医学部, 教授 (40305470)

Co-Investigator(Kenkyū-buntansha) UMEMURA Satoshi  Yokohama City University, 医学部, 教授 (00128589)
GOSHIMA Yoshiro  Yokohama City University, 医学部, 教授 (00153750)
TOYA Yoshiyuki  Yokohama City University, 医学部, 講師 (30237143)
KITAMURA Hitoshi  Yokohama City University, 医学部, 教授 (20094302)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2000: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1999: ¥11,700,000 (Direct Cost: ¥11,700,000)
Keywordsadenylyl cyclase / catecholamine / cAMP / subtype / cardiac subtype / transgenic mice / G protein / sucrose gradient centrifugation / cAMP / 分子生物学
Research Abstract

Recent molecular biological studies have elucidated the presence of multiple adenylyl cyclase isoforms that are different in tissue distribution and biochemical properties. These studies have indicated that the amount and subtype of adenylyl cyclase expressed in each tissue is a key determinant of cAMP signal within the organ. In particular, the heart expresses the two major isoforms of adenylyl cyclase, named type V and VI.We previously demonstrated that these isoforms mostly contribute to the cAMP signal through catecholamine receptors. We have investigated the significance of having multiple adenylyl cyclase isoforms and the distribution of this enzyme within the cell. We have also investigated the biochemical properties of this enzyme using multiple techniques. We have also developed a single step, sucrose gradient centrifugation method that enables easy detection of adenylyl cyclase. Furthermore, we have developed an animal model with deficient adenylyl cyclase type V.During this grant period, we have successfully developed homozygous animals, which will be available to our future studies.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ishikawa,Y: "Immunodetection of adenylyl cyclase protein in tissues"Mol.Cell.Endocrinol. 162. 107-112 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawabe J: "Changes in cavedin subtype protein expression in aging rat organs"Mol Cell Endocrinol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 石川義弘: "神経性循環調節機構の分子生物学"Therapeutic Res. 21. 764-774 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 石川義弘: "心不全治療に於ける自律神経の役割"Therapeutic Res. 21. 2894-2903 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 石川義弘: "カテコラミンと心機能"血管医学. 1. 107-112 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishikawa Y, B Seth Grant, Okumura S, Schwencke C, and Yamamoto M: "Immunodetection of adenylyl cyclase protein in tissues."Mol.Cell.Endocrinol.. 162. 107-112 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawabe J, Grant S B, Yamamoto M, Schwencke C, Okumura S, and Ishikawa Y: "Changes in caveolin subtype protein expression in aging rat organs"Mol.Cell.Endocrinol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishikawa,Y: "Immunodetection of adenylyl cyclase protein in tissues"Mol.Cell.Endocrinol. 162. 107-112 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawabe J: "Changes in caveolin subtype protein expression in aging rat organs"Mol.Cell.Endocrinol. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 石川義弘: "神経性循環調節機構の分子生物学"Therapeutic Res. 21. 764-774 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 石川義弘: "心不全治療に於ける自律神経の役割"Therapeutic Res. 21. 2894-2903 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 石川義弘: "カテコラミンと心機能"血管医学. 1. 107-112 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishikawa,Y: "Exepression of aderylyl cyclase isoform protein in tissues"Mol.Cell Endoerinol.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishikawa,Y: "Diversity of adenylyl cyclase isforms and their regulation in the hesif"J.Cardiovase.Pharmacol.. 34. 3-5 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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