| Project/Area Number |
11480181
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | YOKOHAMACITY UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
ISHIKAWA Yoshihiro Yokohama City University, 医学部, 教授 (40305470)
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| Co-Investigator(Kenkyū-buntansha) |
UMEMURA Satoshi Yokohama City University, 医学部, 教授 (00128589)
GOSHIMA Yoshiro Yokohama City University, 医学部, 教授 (00153750)
TOYA Yoshiyuki Yokohama City University, 医学部, 講師 (30237143)
KITAMURA Hitoshi Yokohama City University, 医学部, 教授 (20094302)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2000: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1999: ¥11,700,000 (Direct Cost: ¥11,700,000)
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| Keywords | adenylyl cyclase / catecholamine / cAMP / subtype / cardiac subtype / transgenic mice / G protein / sucrose gradient centrifugation / cAMP / 分子生物学 |
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| Research Abstract |
Recent molecular biological studies have elucidated the presence of multiple adenylyl cyclase isoforms that are different in tissue distribution and biochemical properties. These studies have indicated that the amount and subtype of adenylyl cyclase expressed in each tissue is a key determinant of cAMP signal within the organ. In particular, the heart expresses the two major isoforms of adenylyl cyclase, named type V and VI.We previously demonstrated that these isoforms mostly contribute to the cAMP signal through catecholamine receptors. We have investigated the significance of having multiple adenylyl cyclase isoforms and the distribution of this enzyme within the cell. We have also investigated the biochemical properties of this enzyme using multiple techniques. We have also developed a single step, sucrose gradient centrifugation method that enables easy detection of adenylyl cyclase. Furthermore, we have developed an animal model with deficient adenylyl cyclase type V.During this grant period, we have successfully developed homozygous animals, which will be available to our future studies.
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