| Project/Area Number |
11480211
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Nagoya University (2000)
Nara Institute of Science and Technology (1999) |
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Principal Investigator |
KAIBUCHI Kozo School of Medicine, Nagoya University, Professor, 医学部, 教授 (00169377)
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| Co-Investigator(Kenkyū-buntansha) |
AMANO Mutsuki Nara institute of Science and Technology, Biological Sciences, Research Associate, バイオサイエンス研究科, 助手 (90304170)
稲垣 直之 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教授 (20223216)
門田 裕志 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助手 (10294282)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2000: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1999: ¥9,100,000 (Direct Cost: ¥9,100,000)
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| Keywords | Small GTPases / Rho-kinase / IQGAP1 / cadherin / cell migration / cell adhesion / Phosphorylation / CRMP-2 / Rho-kinase / IQGAP / 細胞質分裂 |
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| Research Abstract |
The Rho family GTPases (Rho, Rac, and Cdc42) regulate cytoskeletons and cell adhesions through their specific effectors. However, the molecular mechanisms by which the Rho family GTPases regulate these cellular functions have been unknown. Recently, we found that Rho-kinase, an effector of Rho, regulates actin cytoskeleton by regulating the phosphorylation state of myosin light chain (MLC) and that IQGAP1, an effector of Rac and Cdc42, regulates cadherin-mediated cell-cell adhesion. The aim of the present study is to further clarify the mechanism how Rho family GTPases and their effectors regulate cytoskeletons and cell adhesions. Here we found several novel substrates of Rho-kinase : CRMP-2, adducin, MAP2, tau, calponin and EF-1α. CRMP-2 phosphorylation by Rho-kinase was involved in LPA-induced growth cone collapse. We also found that adducin phosphorylation by Rho-kinase modulated cell migration. The analysis of the physiological functions of their phosphorylations is on going. In addition, we demonstrated that the Cdc42/Rac/IQGAP1 system was involved in cell-cell dissociation during cell scattering through α-catenin relocalization. The results obtained here lead us to better understanding how Rho family GTPases regulate cytoskeletons and cell
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