• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of asymmetric division in development

Research Project

Project/Area Number 11480219
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTohoku University

Principal Investigator

MATSUZAKI Fumio  Institute of Development, Aging and Cancer, Department of Developmental Neurobiology, Tohoku University, Professor, 加齢医学研究所, 教授 (10173824)

Co-Investigator(Kenkyū-buntansha) FUSE Naoyuki  Institute of Development, Aging and Cancer, Department of Developmental Neurobiology, Tohoku University, Assistant professor, 加齢医学研究所, 助手 (80321983)
OHSHIRO Tomokazu  Institute of Development, Aging and Cancer, Department of Developmental Neurobiology, Tohoku University, Assistant professor, 加齢医学研究所, 助手 (40311568)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 2000: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1999: ¥9,600,000 (Direct Cost: ¥9,600,000)
Keywordsasymmetric division / neural stem cell / miranda / tumor suppressor gene / neurogenesis / cell polarity / epithelial cell
Research Abstract

Asymmetric division is a basic mechanism for generating cellular diversity during development, and is often achieved by the asymmetric partition of cell fate determinants into one of two daughter cells. The Drosophila neural stem cells, neuroblasts, have been studied as a model system with which to investigate key aspects of this process, given that neural fate determinants such as Numb and Prospero are segregated into one of the two daughter cells generated by neuroblast division.
Two important processes associated with the asymmetric division of neuroblasts are (1) the asymmetric localization of cell fate determinants, which is achieved by specific adapter proteins such as Miranda that themselves localize asymmetrically to the cortex, and (2) the orientation of the mitotic spindle and its coordination with the polarized localization of the determinants, which require the apical Baz-Insc-Pins complex.
To understand the cell polarity underlying the asymmetric division of neuroblasts, we have screened for mutations that affect the localization of Miranda, and have revealed a previously unidentified mechanism responsible for the asymmetric localization of all known cell fate determinants during neuroblast division. This mechanism involves a cascade of two cortical tumor suppressor proteins, Lethal (2) giant larvae (Lgl) and Lethal (1) discs large (Dlg), and occurs upstream of the first and independently of the second of these two aspects of asymmetric division. Lgl and Dlg create intrinsic differences between sibling cells by mediating differential cortical protein targeting during mitosis, demonstrating for the first time a link between these tumor suppressor proteins and the asymmetric division of neural stem cells.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Ohshiro,T.: "Role of tumor suppresser proteins in asymmetric division of Drosophila neuroblast."Nature. 408. 593-596 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 松崎文雄: "Molecular Medicine37巻、臨時増刊:ゲノム時代の脳神経医学"中山書店. 196-203 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohshiro, T., Yagami, T, Zhang, C.and Matsuzaki, F.: "Role of cortical tumor suppressor proteins in asymmetric division of Drosophila neuroblast."Nature. 408. 593-596 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuzaki, F.: "Asymmetric division of Drosophila neural stem cells : a basis for neural diversity."Curr.Opin.Neurobiol.. 10. 38-44 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohshiro,T.: "Role of tumor suppresser proteins in asymmetric division of Drosophila neuroblast."Nature. 408. 593-596 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松崎文雄: "Molecular Medicine37巻、臨時増刊:ゲノム時代の脳神経医学"中山書店. 196-203 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsuzaki F.: "Asymmetric division of Drosophila neural stem cells: a basis for neural diversity"Curr .Opin. Neurobiol.. 10. 38-44 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松崎文雄: "実験医学17巻,No.3,増刊:神経形成と神経機能にかかわる転写因子"羊土社. 7 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松崎文雄: "実験医学17巻,No.16,増刊:神経形成と神経機能にかかわる転写因子"羊土社. 8 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi