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Research on Development and Sex-Differentiation of Mouse Germ Cells

Research Project

Project/Area Number 11480223
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAKATSUJI Norio  Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (80237312)

Co-Investigator(Kenkyū-buntansha) SAITO Tetsuichiro  Kyoto University, Institute for Frontier Medical Sciences, Associate Professor, 再生医科学研究所, 助教授 (00202078)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2001: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2000: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥7,400,000 (Direct Cost: ¥7,400,000)
KeywordsPrimordial germ cells / mouse embryo / meiosis / sex defferentiation / testis / ovary / oocytes / cell death / 遺伝子発現 / 転写因子
Research Abstract

Mouse primordial germ cells (PGCs) migrate from the base of allantois to the genital ridge. They proliferate during the migration and after the arrival until initiation of the sex-differentiation of fetal gonads. Then, PGCs enter into the prophase of the first meiotic division in the ovary to become oocytes, while those in the testis become mitotically arrested to become prospermatogonia. Growth regulation of mouse PGCs has been studied by culturing them on feeder cells. They show a limited period of proliferation in vitro and go into the growth arrest. In the presence of multiple growth signals, however, PGCs can restart rapid proliferation and transform into the pluripotent embryonic germ (EG) cells. We developed a two-dimensional culture system in which we examined transition from the mitotic PGCs into the leptotene stage of the first meiotic division by using the meiosis-specific markers, Scp3 and Dmcl. Such entry into meiosis seems to be programmed in PGCs before reaching the genital ridges and unless inhibited by putative signals from the testicular somatic cells. In such culture system, addition of the leukemia inhibitory factor (LIF) and related ligands caused a strong suppression of the Scp3 expression and meiotic entry by PGCs, indicating that the gp130-mediated signaling may be involved in such inhibition.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Kanno, Y., Tamura, M., Chuma, S., Sakurai, T., Machida, T., Nakatsuji, N.: "A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation"Int. J. Dev. Biol.. 43. 777-784 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Chuma, S., Nakatsuji, N.: "Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp130-mediated signaling"Devel. Biol.. 229. 468-479 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakatsuji, N., Chuma, S.: "Differentiation of mouse primordial germ cells into female or male germ cells"Int. J. Dev. Biol.. 45. 541-548 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tamura, M., Kanno, Y., Chuma, S., Saito, T., Nakatsuji, N.: "Pod-1/Capsulin shows a sex-and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-1"Mech. Devel.. 102. 135-144 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中馬新一郎, 中辻憲夫: "胎仔生殖系列細胞の発生と操作"遺伝子医学. 4. 264-268 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中辻憲夫: "「現代医学の基礎」第5巻「生殖と発生」(編者:森崇英・山村研一)"株式会社 岩波書店. 214 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanno, Y, Tamura, M., Chuma, S, Sakurai, T, Machida, T. and Nakatsujj, N: "A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation"Int. J. Dev. Biol. 43. 777-784 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Chuma, S. and Nakatsuji. N: "Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp130-mediated signaling"Devel. Biol. 229. 468-479 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakatsuji, N. and Chuma, S: "Differentiation of mouse primordial germ cells into female or male germ cells"Int. J. Dev. Biol. 45. 541-548 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tamura, M., Kanno, Y., Chuma, S., Saito, T., and Nakatsuji. N: "Pod 1/Capsulin shows a sex- and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-1"Mech. Devel. 102. 135-144 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Chuma, S. and Nakatsuji. N: "Development and Manipulation of fetal germ-line cells"Idenshi-igaku. 4. 264-268 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakatsuji, N: "Iwanami""Current Medicine" vol. 5 "Reproduction and Development" (editors : Mori, T. and Yamamura,K). 214 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tamura, M., Kamo, Y., Chuma, S., Saito, T., Nakatsuji, N.: "Pod-1/Capsulin shows a sex-and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-1"Mech. Devel.. 102. 135-144 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakatsuji, N. Chuma, S.: "Differentiation of mouse primordial germ cells into female or male germ cells"Int. J. Dev. Biol.. 45. 541-548 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanno,Y.,Tamura,M.,Chuma,S.,Sakurai,T.,Machida,T.and Nakatsuji,N.: "A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation."Int.J.Dev.Biol.. 43. 777-784 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 有馬新一郎,中辻憲夫: "胎仔生殖系列細胞の発生と操作"遺伝子医学. 4. 264-268 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Chuma,S.and Nakatsuji,N: "Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp130-mediated signaling."Devel.Biol.. 229. 468-479 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakatsuji,N.and Chuma,S.: "Differentiation of mouse primordial germ cells into female or male germ cells."Int.J.Dev.Biol.. (In Press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tamura,M.,Kanno,Y.,Chuma,S.,Wakana,S.,Saito,T.,Shirayoshi,Y.and Nakatsuji,N.: "A basic helix-loop-helix transcription factor gene, nephgonadin, isolated by differential screening of the male and female fetal gonads shows a sex-and stagedependent expression pattern in the gonad development."Mech.Devel.. (In Press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yasuhiko KANNO 他: "A cystatin-related gene, testatin/eresp, Shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation"International Journal of Developmental Biology. 43. 777-784 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中辻憲夫: "始原生殖細胞"岩波講座 「現代医学の基礎」. 第5巻. 2-8 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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