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Is GABA excitatory or inhibitory in the developing brain?

Research Project

Project/Area Number 11480239
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionYamaguchi University

Principal Investigator

NAKAMURA Shoji  Yamaguchi university, School of Medicine, Professor, 医学部, 教授 (80112051)

Co-Investigator(Kenkyū-buntansha) ISHIDA Yoshiyuki  Yamaguchi university, School of Medicine, Assistant, 医学部, 助手 (20325210)
FUJIOKA Takashi  Yamaguchi university, School of Medicine, Assistant, 医学部, 助手 (50304473)
SAKATA Yoshiyuki  Yamaguchi university, School of Medicine, Assistant Professor, 医学部, 講師 (10034927)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1999: ¥13,100,000 (Direct Cost: ¥13,100,000)
Keywordsfetal brain / superior colliculus / intracellular Cl^- / GABA / optical recording / fluorescent dye / umbilical cord occlusion / hypoxia / CIイオン / 発達脳 / in vivo / 胎仔
Research Abstract

In the superior colliculus (SC) of the fetal rat brain, changes in intracellular Cl^- following focal stimulation of the SC were examined by in vivo optical recordings with Cl^- sensitive fluorescent dye. In addition, the effect of hypoxia on changes in intracellular Cl^- induced by focal SC stimulation was also examined in fetal rats which were connected with the dams by the umbilical cord. Pregnant rats were anesthetized with urethane (1.3g/kg, i.p.), the uterus was exposed by cesarean section, and partially cut to exposure of the fetus. The fetal head was attached to a stereotaxic apparatus with dental cement, and the surface of the SC was exposed. Stimulating electrodes were implanted in the rostral part of the SC.Cl^- sensitive dye was applied to the surface of the SC for optical recording of intracellular Cl^-. Fetal asphyxia was brought about by occluding the umbilical cord with a fine clip, while the recovery of the umbilical blood flow was attained by local application of papa … More verine, a relaxant of blood vessels, to the occlusion site.
Focal SC stimulation increased intracellular Cl^-. This response was found to extend caudally to the entire SC from the stimulating site. In a total of 25 fetuses examined, 16 revealed Cl^- influx, 1 Cl^- efflux, and 2 both Cl^- influx and efflux following focal SC stimulation. The remaining 6 showed no change in intracellular Cl^-. Furthermore, a total of 12 fetuses were examined to assess the effect on the SC stimulation induced changes in intracellular Cl^- of hypoxia induced by umbilical cord occlusion. Before umbilical cord occlusion, 8 fetuses showed Cl^- influx following focal SC stimulation. However, 3 hours after the occlusion, the number of fetuses showing Cl^- influx was only one, and 10 fetuses revealed both Cl^- influx and efflux. The changes in intracellular Cl^- were attenuated by application of the GABA antagonist bicuculline in all cases (n=5) before umbilical cord occlusion and in 2 of 8 cases after the occlusion. These results suggest that following hypoxia Cl^- influx induced by focal SC stimulation is converted to Cl^- efflux. In addition, the changes in intracellular Cl^- are thought to occur via GABA receptors. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Hashimoto,M.: "Modulating effects of prenatal stress on hyperthermia induced in adult rat offspring by immunological and non-immunological stressors."Physiol.& Behavi.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujioka,T.: "Mild prenatal stress enhances learning performance in the non-adopted rat offspring."Neuroscience. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakata,Y.: "In vivo electrical activity of brainstem neurons in fetal rats during asphyxia."Brain Res.. 871. 271-280 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Iqbal Chowdhury,G.M.: "Induction and adaptation of Fos expression in the rat brain by two types of acute restraint"Brain Res.Bull.. 52. 171-182 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tan,N.: "Involvement of central β-adrenoceptors in the tachycardia induced by water immersion stress in rats."Physiol.& Behavi.. 68. 291-297 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujioka,T.: "The effects of prenatal stress on the development of hypotharamic paraventricular neurons in fetal rats."Neuroscience. 92. 1079-1988 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashimoto, M.: "Modulating effects of prenatal stress on hyperthermia induced in adult rat offspring by immunological and non-immunological stressors."Physiol.& Behave.. (in press ). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujioka, T.: "Mild prenatal stress enhances learning performance in the non-adopted rat offspring."Neuroscience. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakata, Y.: "In vivo electrical activity of brainstem neurons in fetal rats during asphyxia."Brain Res.. 871. 271-280 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tan, N.: "Involvement of central β-adrenoceptors in the tachycardia induced by water immersion stress in rats."Physiol.& Behavi.. 68. 291-297 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Iqbal Chowdhury, G.M.: "Induction and adaptation of Fos expression in the rat brain by two types of acute restraint stress."Brain Res.Bull.. 52. 171-182 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujioka, T.: "The effects of prenatal stress on the development of hypothalamic paraventricular neurons in fetal rats."Neuroscience. 92. 1079-1988 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashimoto,M.: "Modulating effects of prenatal stress on hyperthermia induced in adult rat offspring by immunological and non-immunological stressors."Physiol.& Behavi.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujioka,T.: "Mild prenatal stress enhances learning performance in the non-adopted rat offspring."Neuroscience. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakata,Y.: "In vivo electrical activity of brainstem neurons in fetal rats during asphyxia."Brain Res.. 871. 271-280 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishiike,S.: "Cholinergic influence on vestibular stimulation-induced locus coeruleus inhibition in rats."Acta Otolaryngol.. 120. 404-409 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tan,N.: "Involvement of central β-adrenoceptors in the tachycardia induced by water immersion stress in rats."Physiol.& Behavi.. 68. 291-297 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Iqbal Chowdhury,G.M.: "Induction and adaptation of Fos expression in the rat brain by two types of acute restraint stress."Brain Res.Bull.. 52. 171-182 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Iqbal chowdhury, G. M.: "Induction and adaptation of Fos expression in the rat brain by two types of acute restraint atress."Brain Res. Bull.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Tan, N.: "Involvement of central β-adrenoceptors in the tachycardia induced by water immersion stress in rats."Physiol. & Behav.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe, T.: "Angiotensin AT1 receptors in the preoptic area negatively modulate the cardiovascular and ACTH responses induced in rats by intrapreoptic injection of prostaglandin E2."Brain Res.. 852. 92-99 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujioka, T.: "The effects of prenatal strss on the development of hyothalamic paraventricular neurons in fetal rats."Neuroscience. 92. 1079-1088 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe, T.: "Effects of targeted disruption of the mouse angiotensin II type 2 receptor gene on stress-induced hyperthermia."J. Physiol.. 515. 881-885 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe, T.: "Glucocorticoid enhances IL-1 induced pressor response in freely moving rats through its effecet on nitric oxide release."J. Pharmacol. Exp. Ther.. 289. 24-30 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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